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公开(公告)号：EP2814941A1
公开(公告)日：2014-12-24
申请号：EP13748545.4
申请日：2013-02-15
申请人： Medicago Inc.
CPC分类号： A01G7/06 , A01G7/00 , A01G22/00 , C12N15/8205
摘要： It is provided a plant infiltration device for infiltrating plants with an inoculum and method of using same, the device comprising a frame having one or more tray racks adapted for receiving at least one tray of plants to be infiltrated; an automated manipulation mechanism mounted to the frame and operable to displace the plant trays within the device; a plurality of inoculum reservoirs mounted to the frame and containing one or more inoculum fluids; an infiltration tank mounted to the frame and within which the plant trays are received when disposed in the infiltration position; and a vacuum generating device in communication with the infiltration tank to apply a negative pressure within the infiltration tank, thereby opening pores of the plant leaves immersed in the inoculum within the infiltration tank and causing the inoculum fluid to infiltrate into the plant leaves.
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公开(公告)号：EP2854509B1
公开(公告)日：2016-07-27
申请号：EP13797900.1
申请日：2013-05-28
申请人： Medicago Inc.
CPC分类号： A01G9/02 , A01G9/0297
摘要： A plant tray for receiving and retaining one or more plant pots includes a plant-receiving tray for use in a high-throughput plant infiltration process used to produce recombinant proteins. The plant tray described has a base retaining tray with an upper surface defining cavities for receiving the plant pots, and a top plate displaceably mounted on the base retaining tray and having apertures, the apertures defining a channel with the corresponding cavities of the bottom tray when aligned. The top plate is displaceable between a locked operative position wherein the apertures and the corresponding cavities are partially misaligned, thereby locking the plant pots in between the top plate and the base retaining tray.
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公开(公告)号：EP3167057A1
公开(公告)日：2017-05-17
申请号：EP15819391.2
申请日：2015-07-10
申请人： Medicago Inc. , Université Laval
发明人： MICHAUD, Dominique , PEPIN, Steeve , ETHIER, Gilbert , GOULET, Marie-Claire , GAUDREAU, Linda , GAGNE, Marielle , MARTEL, Michele , BECHTOLD, Nicole , D'AOUST, Marc-Andre , GOSSELIN, Andre
CPC分类号： C12N15/8251 , A01H3/00 , A01H3/02 , A01H3/04 , C12N15/8258
摘要： A method for synthesizing a protein of interest within a plant or a portion of a plant is provided. The method involves treating the plant to increase secondary leaf biomass production, followed by introducing one or more than one nucleic acid sequence encoding a protein of interest operatively linked with a regulatory region that is active in the plant, into the plant. The plant is then maintained under conditions that permit the nucleic acid sequence encoding the protein of interest to be expressed in the plant or the portion of the plant. Optionally, the plant or portion of the plant may be harvested and the protein of interest extracted.
摘要翻译： 提供了在植物或植物的一部分内合成感兴趣的蛋白质的方法。 该方法包括处理植物以增加次生叶生物量的产生，随后将编码目的蛋白质的一种或多于一种的核酸序列与在植物中具有活性的调节区域可操作地连接到植物中。 然后将植物保持在允许编码目的蛋白质的核酸序列在植物或植物部分中表达的条件下。 任选地，可以收获植物或植物的一部分，并提取目的蛋白质。
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公开(公告)号：EP3167057B1
公开(公告)日：2020-03-25
申请号：EP15819391.2
申请日：2015-07-10
申请人： Medicago Inc. , Université Laval
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公开(公告)号：EP2854509A1
公开(公告)日：2015-04-08
申请号：EP13797900.1
申请日：2013-05-28
申请人： Medicago Inc.
IPC分类号： A01G9/02
CPC分类号： A01G9/02 , A01G9/0297
摘要： A plant tray for receiving and retaining one or more plant pots includes a plant-receiving tray for use in a high-throughput plant infiltration process used to produce recombinant proteins. The plant tray described has a base retaining tray with an upper surface defining cavities for receiving the plant pots, and a top plate displaceably mounted on the base retaining tray and having apertures, the apertures defining a channel with the corresponding cavities of the bottom tray when aligned. The top plate is displaceable between a locked operative position wherein the apertures and the corresponding cavities are partially misaligned, thereby locking the plant pots in between the top plate and the base retaining tray.
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公开(公告)号：EP2171068A1
公开(公告)日：2010-04-07
申请号：EP08772809.3
申请日：2008-06-13
申请人： Medicago Inc.
发明人： D'AOUST, Marc-Andre , BELLES-ISLES, Julie , BECHTOLD, Nicole , MARTEL, Michele , LAVOIE, Pierre-Olivier , VEZINA, Louis-Philippe
CPC分类号： C12N15/8257 , A01H3/00 , A01H3/04 , C12N9/1051 , C12N15/8216 , C12N15/8218 , C12N15/8258
摘要： A method for synthesizing a protein of interest within a plant or a portion of a plant is provided. The method involves introducing one or more than one nucleic acid sequence encoding a protein of interest operatively linked with a regulatory region obtained from a photosynthetic gene that is active in the plant, in a transient manner. The plant is then maintained under conditions that permit the nucleic acid sequence encoding the protein of interest to be expressed in the plant or the portion of the plant. The plant may be pruned prior to the introducing one or more than one nucleic acid sequence.
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