公开(公告)号：EP1805324A2

公开(公告)日：2007-07-11

申请号：EP05792245.2

申请日：2005-10-10

申请人： Medical Research Council ,  The President and Fellows of Harvard College

发明人： GRIFFITHS, Andrew Medical Research Council ,  WEITZ, David ,  LINK, Darren ,  AHN, Keunho ,  BIBETTE, Jerome

IPC分类号： C12Q1/68

CPC分类号： B01F3/0807 ,  B01F5/0646 ,  B01F5/0647 ,  B01F5/0655 ,  B01F13/0062 ,  B01F13/0071 ,  B01F13/0076 ,  B01J19/0046 ,  B01J2219/00466 ,  B01J2219/00468 ,  B01J2219/005 ,  B01J2219/0052 ,  B01J2219/00545 ,  B01J2219/00576 ,  B01J2219/00657 ,  B01J2219/00722 ,  B01L3/502746 ,  B01L3/502753 ,  B01L3/502776 ,  B01L3/502784 ,  B01L2200/0636 ,  B01L2200/0673 ,  B01L2300/0654 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2400/0415 ,  B01L2400/0487 ,  C12N15/1058 ,  C12N15/1075 ,  C12P21/00 ,  C12Q1/6874 ,  G01N15/1459 ,  G01N15/1484 ,  G01N2015/149

摘要： The invention describes a method for isolating one or more genetic elements encoding a gene product having a desire activity, comprising the steps of: (a) compartmentalising genetic elements into microcapsules; and (b) sorting the genetic elements which express the gene product having the desired activity; wherein at least one step is under microfluidic control. The invention enables the in vitro evolution of nucleic acids and proteins by repeated mutagenesis and iterative applications of the method of the invention.