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    EXPRESSION VECTOR FOR PSEUDONOCARDIA AUTOTROPHICA
    2.
    发明公开
    EXPRESSION VECTOR FOR PSEUDONOCARDIA AUTOTROPHICA 有权
    表达者FÜRPSEUDONOCARDIA AUTOTROPHICA

    公开(公告)号：EP2341137A1

    公开(公告)日：2011-07-06

    申请号：EP09819153.9

    申请日：2009-10-05

    申请人： MERCIAN CORPORATION ,  National Institute of Advanced Industrial Science and Technology

    发明人： FUJII Yoshikazu ,  FUJII Tadashi ,  ARISAWA Akira ,  TAMURA Tomohiro

    IPC分类号： C12N15/09 ,  C12N1/21 ,  C12P33/06 ,  C12N9/02

    CPC分类号： C12P21/02 ,  C12N9/0071 ,  C12N9/0073 ,  C12N15/74 ,  C12P7/02 ,  C12P7/62 ,  C12P17/06 ,  C12Y114/13013

    摘要： An expression vector capable of expressing a foreign gene in Pseudonocardia autotrophica; a transformant of Pseudonocardia autotrophica produced by using the expression vector; a method for producing a protein by using the transformant; a method for producing an active form of vitamin D3 from vitamin D3, which comprises highly expressing a gene encoding an enzyme involved in the synthesis of the active form of vitamin D3 in a transformant by using the expression vector or the transformant; a method for producing 25-hydroxyvitamin D2 from vitamin D2; and a method for producing pravastatin from compactin, which comprises highly expressing a compact in hydroxylase gene in a transformant by using the expression vector or the transformant.

    摘要翻译： 能够表达外源基因的表达载体，其能够在自养假诺卡氏菌中表达; 通过使用表达载体产生的假心内膜自养基因的转化体; 使用该转化体生产蛋白质的方法; 从维生素D3生产活性形式的维生素D3的方法，其包括通过使用表达载体或转化体高度表达编码参与转化体中活性形式的维生素D3的活性形式的酶的基因; 从维生素D2生产25-羟基维生素D 2的方法; 以及通过使用表达载体或转化体在转化体中高度表达羟化酶基因中的紧密度的方法，从卡培生制备普伐他汀的方法。

    IMPROVED ß-FRUCTOFURANOSIDASE
    3.
    发明公开
    IMPROVED ß-FRUCTOFURANOSIDASE 审中-公开
    VERBESSERTE SS-FRUCTOFURANOSIDASE

    公开(公告)号：EP3088520A1

    公开(公告)日：2016-11-02

    申请号：EP14874041.8

    申请日：2014-12-26

    申请人： B Food Science Co., Ltd. ,  MicroBiopharm Japan Co., Ltd.

    发明人： TOCHIO Takumi ,  NAKAMURA Saki ,  YAHARA Misa ,  FUJII Tadashi ,  TAMURA Keisuke

    IPC分类号： C12N15/09 ,  C12N1/21 ,  C12N9/24 ,  C12P19/00

    CPC分类号： C12N9/2431 ,  C12P19/14 ,  C12Y302/01026

    摘要： [Problem]
    To provide an improved β-fructofuranosidase which enables the production of kestose with high efficiency while reducing the amount of a by-product such as nystose produced during the production of kestose.
    [Solution]
    An improved β-fructofuranosidase comprising an amino acid sequence which is produced by introducing an amino acid mutation into an amino acid sequence for a β-fructofuranosidase having 60% or higher identity to the amino acid sequence for wild-type β-fructofuranosidase which is represented by SEQ ID NO: 2, wherein the amino acid mutation is such a mutation that, when amino acid sequence alignment is performed, a histidine (H) residue corresponding to the position as numbered from the N-terminal of the amino acid sequence for the wild-type β-fructofuranosidase which is represented by SEQ ID NO: 2 can be replaced by an arginine (R) residue or a lysine (K) residue.

    摘要翻译： [问题]提供改进的β-呋喃果糖苷酶，其能够高效率地生产睾丸酮，同时减少副产物的量，例如在生产睾丸酮期间产生的糖。 [解决方案]改进的β-呋喃果糖苷酶包含通过将氨基酸突变引入氨基酸序列而产生的氨基酸序列，所述氨基酸序列与野生型呋喃果糖苷酶的氨基酸序列具有60％或更高同一性的2-呋喃果糖苷酶 其由SEQ ID NO：2表示，其中氨基酸突变是这样的突变，当进行氨基酸序列比对时，对应于氨基酸的N末端的位置的组氨酸（H）残基 由SEQ ID NO：2表示的野生型2-呋喃果糖苷酶的序列可以被精氨酸（R）残基或赖氨酸（K）残基所代替。

    IMPROVED BETA-FRUCTOFURANOSIDASE
    4.
    发明公开
    IMPROVED BETA-FRUCTOFURANOSIDASE 审中-公开
    改进的β-FRUCTOFURANOSIDASE

    公开(公告)号：EP3269808A1

    公开(公告)日：2018-01-17

    申请号：EP16761839.6

    申请日：2016-03-10

    申请人： B Food Science Co., Ltd. ,  MicroBiopharm Japan Co., Ltd.

    发明人： TOCHIO Takumi ,  ITO Naomi ,  NAKAMURA Saki ,  FUKATANI Yoshimi ,  FUJII Tadashi ,  TAMURA Keisuke

    IPC分类号： C12N15/09 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12N9/24 ,  C12P19/14 ,  C12P21/02

    CPC分类号： C12N9/2431 ,  C12N15/09 ,  C12P19/14 ,  C12P21/02 ,  C12Y302/01026

    摘要： [Problem]
    To provide an improved β-fructofuranosidase which is capable of efficiently producing kestose while inhibiting the production of nystose.
    [Solution]
    This improved β-fructofuranosidase comprises either: an amino acid sequence (a) obtained by introducing, into the amino acid sequence represented by SEQ ID NO: 2, an amino acid mutation i) in which the 85th glycine (G) from the N-terminal is substituted for a protein-constituting amino acid other than glycine (G), and/or an amino acid mutation ii) in which the 310th histidine (H) from the N-terminal is substituted for lysine (K), arginine (R), or tyrosine (Y); or an amino acid sequence (b) which exhibits β-fructofuranosidase activity, and which comprises an amino acid sequence obtained by deleting, substituting, inserting, or adding one or more amino acids in (a) other than the amino acid into which the amino acid mutation has been introduced.

    摘要翻译： 本发明提供一种能够在抑制芽孢杆菌生成的同时有效地生产酮酯糖的改良型β-呋喃果糖苷酶。 该改良的β-呋喃果糖苷酶含有：在序列号2所示的氨基酸序列中导入了来自第85位的甘氨酸（G）的氨基酸突变i） N-末端取代除甘氨酸（G）以外的蛋白质构成氨基酸，和/或ii）来自N-末端的第310个组氨酸（H）取代赖氨酸（K）的氨基酸突变ii） 精氨酸（R）或酪氨酸（Y）; 或显示β-呋喃果糖苷酶活性的氨基酸序列（b），并且包含通过缺失，取代，插入或添加（a）中的一个或多个氨基酸而获得的氨基酸序列，所述氨基酸不同于氨基酸 酸性突变已被引入。