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公开(公告)号:EP2341137B1
公开(公告)日:2014-08-13
申请号:EP09819153.9
申请日:2009-10-05
申请人: MicroBiopharm Japan Co., Ltd. , National Institute of Advanced Industrial Science and Technology
发明人: FUJII Yoshikazu , FUJII Tadashi , ARISAWA Akira , TAMURA Tomohiro
CPC分类号: C12P21/02 , C12N9/0071 , C12N9/0073 , C12N15/74 , C12P7/02 , C12P7/62 , C12P17/06 , C12Y114/13013
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2.
公开(公告)号:EP2341137A1
公开(公告)日:2011-07-06
申请号:EP09819153.9
申请日:2009-10-05
发明人: FUJII Yoshikazu , FUJII Tadashi , ARISAWA Akira , TAMURA Tomohiro
CPC分类号: C12P21/02 , C12N9/0071 , C12N9/0073 , C12N15/74 , C12P7/02 , C12P7/62 , C12P17/06 , C12Y114/13013
摘要: An expression vector capable of expressing a foreign gene in Pseudonocardia autotrophica; a transformant of Pseudonocardia autotrophica produced by using the expression vector; a method for producing a protein by using the transformant; a method for producing an active form of vitamin D3 from vitamin D3, which comprises highly expressing a gene encoding an enzyme involved in the synthesis of the active form of vitamin D3 in a transformant by using the expression vector or the transformant; a method for producing 25-hydroxyvitamin D2 from vitamin D2; and a method for producing pravastatin from compactin, which comprises highly expressing a compact in hydroxylase gene in a transformant by using the expression vector or the transformant.
摘要翻译: 能够表达外源基因的表达载体,其能够在自养假诺卡氏菌中表达; 通过使用表达载体产生的假心内膜自养基因的转化体; 使用该转化体生产蛋白质的方法; 从维生素D3生产活性形式的维生素D3的方法,其包括通过使用表达载体或转化体高度表达编码参与转化体中活性形式的维生素D3的活性形式的酶的基因; 从维生素D2生产25-羟基维生素D 2的方法; 以及通过使用表达载体或转化体在转化体中高度表达羟化酶基因中的紧密度的方法,从卡培生制备普伐他汀的方法。
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