公开(公告)号：EP4036098A1

公开(公告)日：2022-08-03

申请号：EP21154663.5

申请日：2021-02-02

申请人： Miltenyi Biotec B.V. & Co. KG

发明人： MARMA, Sano Mong ,  GUAN, Xiao Pei ,  SOLIMAN, Sameh ,  PERBOST, Michel

IPC分类号： C07H1/00 ,  C07H19/20 ,  C07H19/10

摘要： The invention is directed to a method for production of a deoxynucleotide triphosphate according to general formula (7)

wherein B is a nucleobase and R is an alkyl group having 1 to 4 carbon atoms and n is an integer from 1 to 10 by reaction of reaction precursor of compound 6

wherein R 1 , R 2 and R 3 are independently H, O-Alkyl with alkyl residues having 1 to 4 carbon atoms or halogen and n is an integer from 1 to 10 with dialkyl(alkylthio)sulfonium salt (8B)

wherein R 4 , R 5 R 6 are independently alkyl residues having 1 to 4 carbon atoms and X is selected from the group consisting of BP 4 - , F - , Cl - , Br - , I - , SO 4 2- , SO 3 2- in aqueous solution having a pH between 3 and 7.

公开(公告)号：EP4342998A1

公开(公告)日：2024-03-27

申请号：EP22197285.4

申请日：2022-09-23

申请人： Miltenyi Biotec B.V. & Co. KG

发明人： MARMA, Mong ,  PERBOST, Michel ,  PINARD, Robert ,  GUAN, Xiao-Pei ,  SOLIMAN, Sameh ,  NEIL, Emily

IPC分类号： C12Q1/6816

摘要： The invention is directed to a method for detecting RNA, DNA or protein target sequences by
a) Hybridizing a library of probes having the general formula (I)

         P - (CL-D) x      (I)

With
P: probes having at least 10 nucleotides or amino acids
CL: cleavable linker
D: fluorescent dye
X: integer between 1 and 5

to RNA, DNA or protein target sequences wherein the library comprises probes P having different sequences of nucleotides or amino acids and cleavable linkers CL of different groups which are cleavable with different means

b) Removing unhybridized probes and detecting the hybridized probes via the fluorophores D by a first image
c) Cleaving sequentially by different means each group of chemical linkers CL from the hybridized probes; removing the thus cleaved fluorophores D and detecting the remaining hybridized probes via their fluorophores D by a second image
d) Detecting the removed fluorophores D by comparing the first and second image.
e) Obtaining a part of the sequence information of the target sequences via the sequence information of the probes P associated with the removed fluorophores D
f) Repeating step c) until all groups of chemical linkers CL are cleaved.

公开(公告)号：EP4089098A1

公开(公告)日：2022-11-16

申请号：EP21172924.9

申请日：2021-05-10

申请人： Miltenyi Biotec B.V. & Co. KG

发明人： MARMA, Mong ,  GUAN, Xiao-Pei ,  SOLIMAN, Sameh ,  PERBOST, Michel

IPC分类号： C07H19/10 ,  C07H19/20 ,  C12Q1/68

摘要： The invention is directed to a deoxynucleotide triphosphate comprising a FRET energy transfer dye pair according to formula (I)

wherein B is a nucleobase
R is a reversible capping group
n is an integer from 1 to 10
CL is a chemically cleavable linker
D is a donor fluorescent dye
A is an acceptor dye
FL is a linker group