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    MALDI-MATRIX
    1.
    发明公开
    MALDI-MATRIX 审中-公开

    公开(公告)号：EP1549958A2

    公开(公告)日：2005-07-06

    申请号：EP03793665.5

    申请日：2003-07-30

    申请人： N.V. Nutricia

    发明人： STAHL, Bernd ,  BOEHM, Günther ,  MANK, Marko

    IPC分类号： G01N33/68

    CPC分类号： H01J49/164 ,  G01N33/6848 ,  G01N33/6851 ,  Y10T436/10

    摘要： The invention relates to matrixes for ultraviolet matrix-assisted laser desorption-ionisation mass spectrometry made of the salt of an amine which reacts as a proton acceptor and an organic substance which reacts as a proton donator. Either the amine or the organic substance absorbs UV light. Said matrixes are characterised in that they represent an ionic liquid at room temperature and in that the amine is selected from the group which is made up of 3-aminoquinoline, pyridine, a primary amine whose N-Atom is bound to a phenyl radical or a straight or branched, saturated C1-C11-alkyl radical which can be substituted by an OH-group, a secondary and tertiary amine whose N-Atoms are bound to two or three radicals which can be the same or different and represent a straight or branched, saturated C1-C8-alkyl radical which can be substituted by an OH-group and a phenyl radical, imidazole and the C and/or N-alkylated imidazole derivatives and also characterised in that the organic substance is selected from the group which is made up of 2,5-dihydroxybenzoic acid and the isomers thereof, 2-hydroxy-5-methoxy-benzoic acid and the isomers thereof, picolinic-acid, 3-hydroxypicolinic acid, nicotinic acid, 5-chloro-2-mercapto-benzo-thiazole, 6-aza-2-thiothymine, 2',4',6'-trihydroxyacetophenone-monohydrate, 2',6'-di-hydroxyaceto-phenone, 9H-pyridol[3,4-b]indole, dithranole, trans-3-indolacrylic acid, osazones, ferulic acid, 2,5-dihydroxyacetophenone, 1-nitrocarbazole, 7-amino-4-methylcumarine, 2-(p-hdroxyphenylazo)-benzoic acid, 8-aminopyrene-2,3,4-trissulfonic acid, 2-[2E-3-(4-tert-butylphenyl)-2-methylprop-2-enylidene]malonitril (DCTB), 4-methoxy-3-hydroxycinnamic acid and 3,4-dihydroxycinnamic acid. Said liquid matrixes enable reproducible, error-free analysis values to be obtained. The combination of pure mass spectrometric analysis with additional knowledge of enzymatic reactions/modifications with the possible monitoring is also described.

    摘要翻译： 本发明涉及由作为质子受体反应的胺的盐和作为质子供体反应的有机物质制成的用于紫外线基质辅助激光解吸电离质谱的基质。 胺或有机物质吸收紫外线。 所述基质的特征在于它们在室温下代表离子液体，并且胺选自由3-氨基喹啉，吡啶，其N-原子与苯基结合的伯胺或 可以被OH-取代的直链或支链饱和C 1 -C 11烷基，其N-原子与两个或三个可以相同或不同并代表直链或支链的基团的仲胺和叔胺 ，可被OH基团和苯基取代的饱和C 1 -C 8烷基，咪唑和C和/或N-烷基化咪唑衍生物，其特征还在于有机物质选自 2-羟基-5-甲氧基 - 苯甲酸及其异构体，吡啶甲酸，3-羟基吡啶甲酸，烟酸，5-氯-2-巯基 - 苯并 - 噻唑，6-氮杂-2-硫胸腺嘧啶，2'，4'，6'-三羟基乙酰磷 烯酮 - 单水合物，2'，6'-二羟基乙酰苯酮，9H-吡啶并[3,4-b]吲哚，二噻烯，反式-3-吲哚丙烯酸，osazones，阿魏酸，2,5-二羟基苯乙酮， 2-硝基咔唑，7-氨基-4-甲基香豆素，2-（对羟基苯基偶氮） - 苯甲酸，8-氨基芘-2,3,4-三磺酸，2- [2E-3-（4-叔丁基苯基）-2 （DCTB），4-甲氧基-3-羟基肉桂酸和3,4-二羟基肉桂酸。 所述液体基质能够获得可再现的，无误差的分析值。 还描述了纯质谱分析与对酶促反应/修饰的额外知识以及可能的监测的组合。