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    Quantitative peptide analysis by mass spectrometry
    5.
    发明公开
    Quantitative peptide analysis by mass spectrometry 审中-公开
    定量分析测定Massenspektrometrie

    公开(公告)号：EP2530701A3

    公开(公告)日：2017-06-14

    申请号：EP12003722.1

    申请日：2012-05-10

    Applicant: Bruker Daltonik GmbH ,  Anderson Forschung Group LLC

    Inventor： Suckau, Detlev ,  Anderson, Leigh

    IPC: H01J49/00 ,  G01N33/68

    CPC classification number: G01N33/6851 ,  G01N2560/00 ,  H01J49/0004 ,  H01J49/0009 ,  H01J49/0031 ,  H01J49/0036 ,  H01J49/164

    Abstract: The invention relates to an accurate and safe determination of peptide concentrations in solutions by mass spectrometry using internal reference substances, determining the concentration ratio of a target peptide to a reference peptide by forming the ratio of the integrated ion currents of the complete isotope peak groups instead of only comparing single ion peaks. To obtain the integrated ion current of an isotope peak group, the invention applies a curve fitting method, fitting a family of bell-shaped curves with correct isotope distribution to the measured peak groups. The reference peptide must be chemically identical with the target peptide, but isotopically marked. It is a great advantage over other analysis methods, that the curve fitting delivers additionally individual quality indicators for each analysis, proving absence of any superposition of ion peaks of other substances, proving the measurement of the correct target peptide, and proving failure-free instrument adjustment and operation. A further quality confirmation may be obtained by a joint measurement of a reporter fragment ion spectrum of target and reference peptide.

    Abstract translation: 本发明涉及通过使用内部参考物质的质谱法来确定溶液中肽浓度的准确和安全的确定，通过形成完全同位素峰基团的积分离子流的比例来确定靶肽与参照肽的浓度比 只比较单个离子峰。 为了获得同位素峰值的集成离子电流，本发明应用曲线拟合方法，将具有正确同位素分布的钟形曲线族拟合到测量的峰组。 参考肽必须与靶肽化学相同，但同位素标记。 与其他分析方法相比，曲线拟合提供了每个分析的独立质量指标，证明没有其他物质的离子峰的叠加，证明了正确的靶肽的测量，并证明了无故障仪器 调整和运行。 可以通过联合测量靶标和参照肽的报道分子片段离子谱来获得进一步的质量确认。

    MASS SPECTROMETER
    10.
    发明公开
    MASS SPECTROMETER 审中-公开
    质谱分析仪

    公开(公告)号：EP2988316A1

    公开(公告)日：2016-02-24

    申请号：EP14784897.2

    申请日：2014-04-09

    Applicant: Shimadzu Corporation

    Inventor： HARADA, Takahiro

    IPC: H01J49/26 ,  H01J49/10

    CPC classification number: H01J49/164 ,  H01J49/04 ,  H01J49/40

    Abstract: A mass spectrometer (1) is provided with: an ionization chamber (10) for ionizing a sample (S) on its surface at an analysis point through irradiation by a laser beam; an analysis chamber (23) having a mass spectroscope (24) for detecting ions; a middle vacuum chamber (21, 22) arranged between the ionization chamber (10) and the analysis chamber (23); and an introduction pipe (12) or an introduction hole for allowing the inside of the housing (11) of the ionization chamber (10) to communicate with the inside of the middle vacuum chamber (21), wherein ions and fine particles, which have not been drawn into the introduction pipe (12) or introduction hole, can be prevented from spreading inside of the chamber. The structure of the mass spectrometer (1) further includes: an exhaust pipe (13); and a fan (15) for drawing air into the exhaust pipe (13) so that air that contains ions and/or fine particles, which have not been introduced into the introduction pipe (12) or introduction hole, can be suctioned up into the exhaust pipe (13) when the fan (15) is in operation.

    Abstract translation: 质谱仪（1）具备：电离室（10），其通过激光的照射使分析点的表面的试样（S）离子化; 具有用于检测离子的质谱仪（24）的分析室（23） 布置在电离室（10）和分析室（23）之间的中间真空室（21,22）; 以及使电离室（10）的壳体（11）的内部与中间真空室（21）的内部连通的导入管（12）或导入孔，其中离子和微粒具有 没有被吸入导入管（12）或导入孔的气体能够防止向腔室内部扩散。 质谱仪（1）的结构还包括：排气管（13）; 以及用于将空气抽入排气管（13）的风扇（15），使得包含未被引入导入管（12）或导入孔中的离子和/或微粒的空气可被吸入到 排气管（13）在风扇（15）运行时排出。

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