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1.发明公开CELL DIFFERENTIATION ASSAY METHOD, CELL ISOLATION METHOD, METHOD FOR PRODUCING INDUCED PLURIPOTENT STEM CELLS, AND METHOD FOR PRODUCING DIFFERENTIATED CELLS 审中-公开细胞分化测试程序方法隔离牢房，方法生成诱导的多能干细胞及其制造方法的分化细胞
公开(公告)号：EP2821498A4
公开(公告)日：2015-12-09
申请号：EP13755221
申请日：2013-02-27
发明人： TATENO HIROAKI , HIRABAYASHI JUN , ASASHIMA MAKOTO , ITO YUZURU , ONUMA YASUKO , WARASHINA MASAKI , FUKUDA MASAKAZU
IPC分类号： C12Q1/02 , C12M1/00 , C12M1/34 , C12N5/10 , G01N33/569
CPC分类号： G01N33/56966
摘要： Provided are a method for accurately evaluating the differentiation status of stem cells by selectively staining only stem cells in an undifferentiated state, and a method for positively isolating only stem cells in an undifferentiated state. Specifically provided is a method for determining differentiation of a cell comprising a step of contacting a test cell with a probe comprising protein (A) or (B) below and a step of detecting the presence of binding of the probe to the test cell. The method for determining differentiation of a cell is capable of detecting the presence or absence of an undifferentiated stem cell in test cells by using a probe that specifically reacts with undifferentiated stem cells and detecting the presence of bonding to the test cell. (A) A protein comprising an amino acid sequence shown in SEQ ID NO: 1 and recognizing a sugar chain structure of "Fuc±1-2Gal²1-3GlcNAc" and/or "Fuc±1-2Gal²1-3GalNAc;" and (B) a protein comprising an amino acid sequence showing 80% or more similarity to the amino acid sequence shown in SEQ ID NO: 1 and recognizing a sugar chain structure of "Fuc±1-2Gal²1-3GlcNAc" and/or "Fuc±1-2Gal²1-3GalNAc."
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2.发明公开UNDIFFERENTIATED CELL DETECTION METHOD AND COMPLEX CARBOHYDRATE DETECTION METHOD 有权未分化的细胞检测方法和复合碳水化合物检测方法
公开(公告)号：EP2774994A4
公开(公告)日：2015-09-02
申请号：EP12845274
申请日：2012-10-31
发明人： TATENO HIROAKI , HIRABAYASHI JUN , ITO YUZURU , ONUMA YASUKO , ASASHIMA MAKOTO , KUNO ATSUSHI , WARASHINA MASAKI , FUKUDA MASAKAZU
CPC分类号： G01N33/56966 , C07K14/195 , G01N2333/4724 , G01N2400/00
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公开(公告)号：EP2957630A4
公开(公告)日：2016-08-17
申请号：EP14751402
申请日：2014-02-13
发明人： TATENO HIROAKI , ITO YUZURU , ONUMA YASUKO , HIRABAYASHI JUN , ASASHIMA MAKOTO
CPC分类号： C12N5/0081 , C07K14/195 , C07K2319/33 , C07K2319/55 , C12N9/1077 , C12Y204/02036
摘要： An object of the present invention is to provide a method for introducing a target substance into undifferentiated cells, and a carrier therefor, whereby the target substance can be specifically introduced into the undifferentiated cells by contacting the undifferentiated cells with an rBC2LCN-target substance fusion product in which rBC2LCN lectin is fused to the target substance. Particularly, an rBC2LCN-toxin fusion product in which rBC2LCN lectin is fused to a toxin functioning in cells or its domain having the ability to kill cells functions as an agent for eliminating undifferentiated stem cells and can be administered into a medium after inducing the differentiation of the stem cells to reliably kill only the stem cells in an undifferentiated state.
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