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    Process for producing a plant storage organ in which a GLP-1 derivative recombinant protein is highly produced.
    1.
    发明公开
    Process for producing a plant storage organ in which a GLP-1 derivative recombinant protein is highly produced. 审中-公开
    Verfahren zur Herstellung eines pflanzlichen Speicherorgans mit hoher rekrombinant GLP-1 Derivat Produktion

    公开(公告)号：EP2256189A1

    公开(公告)日：2010-12-01

    申请号：EP10174310.2

    申请日：2004-03-26

    申请人： NATIONAL INSTITUTE OF AGROBIOLOGICAL SCIENCES ,  NIPPON PAPER INDUSTRIES CO., LTD. ,  SANWA KAGAKU KENKYUSHO CO., LTD.

    发明人： Sugita, Koichi ,  Kasahara, Saori ,  Ebinuma, Hiroyasu ,  Takaiwa, Humio ,  Jomori, Takahito ,  Hayashi, Yuji ,  Tashita, Akira ,  Kobara, Yukari

    IPC分类号： C12N15/00 ,  A01H5/00 ,  C07K14/605

    CPC分类号： C12N15/8257 ,  C07K14/605 ,  C12N15/8216

    摘要： The present invention provides a method for highly producing a recombinant protein in a plant storage organ and a GLP-1 derivative. The plant storage organ in which the recombinant protein is highly produced is obtained by transformation with the use of a vector which comprises a recombinant protein gene, a cytokinin-related gene, a drug-resistant gene and a removable DNA element, in which the oytohinin-related gene and the drug-resistant gene exist in the positions so that they can behave together with the DNA element, while the recombinant protein to be expressed in the plant storage organ exists in the position so that it would not behave together with the DNA element. The GLP-1 is produced by using the method, and a derivative having been stabilized against enzymatic digestion is further provided.

    摘要翻译： 本发明提供了在植物贮藏器官和GLP-1衍生物中高度产生重组蛋白的方法。 通过使用包含重组蛋白基因，细胞分裂素相关基因，耐药性基因和可除去DNA元件的载体转化获得重组蛋白高度产生的植物贮藏器官，其中奥托列宁 相关基因和耐药基因存在于与DNA元件一起表现的位置，而在植物储存器官中表达的重组蛋白存在于与DNA不一致的位置 元件。 通过使用该方法制备GLP-1，进一步提供了稳定的酶消化衍生物。

    cDNA of human hepatitis C virus, its clone and use thereof
    2.
    发明公开
    cDNA of human hepatitis C virus, its clone and use thereof 失效
    C-DNS von丙型肝炎病毒; seine Klonierung und Verwendung。

    公开(公告)号：EP0541089A2

    公开(公告)日：1993-05-12

    申请号：EP92118974.2

    申请日：1992-11-05

    申请人： SANWA KAGAKU KENKYUSHO CO., LTD.

    发明人： Kurono, Masayasu ,  Mitani, Takahiko ,  Jomori, Takahito ,  Hayashi, Yuji ,  Suzuki, Eiji ,  Sawai, Kiichi

    IPC分类号： C12N15/40 ,  C12N15/51 ,  C12Q1/70 ,  G01N33/569 ,  A61K39/29

    CPC分类号： C07K14/005 ,  A61K39/00 ,  C12N2770/24222

    摘要： There are disclosed a cDNA of human hepatitis C virus gene, its clone and use thereof. The cDNA is prepared by constructing a cDNA library with an RNA, as a template, derived from a liver tissue or serum of a patient who was definitely diagnosed as infected with hepatitis C, carrying out a cDNA cloning with use of the library and probes encoding parts of nucleotide sequence which codes a known HCV virus gene to obtain clones reacting with the probes, carrying out further cloning with use of the reacted clones, determining a nucleotide sequence on each positive clone, and ligating peptides with the nucleotide sequence to establish as only one HCV clone. The HCV clone is constructed with about 9500 nucleotides having a sequence shown in the Sequence Listing.

    摘要翻译： 公开了人丙型肝炎病毒基因的cDNA，其克隆及其用途。 通过构建cDNA文库制备cDNA文库，其中RNA作为模板，衍生自绝对诊断为感染丙型肝炎的患者的肝组织或血清，使用文库和编码的探针进行cDNA克隆 编码已知HCV病毒基因以获得与探针反应的克隆的部分核苷酸序列，进行使用反应的克隆的进一步克隆，确定每个阳性克隆上的核苷酸序列，并将肽与核苷酸序列连接以确定为仅 一个HCV克隆。 构建具有序列表所示序列的大约9500个核苷酸的HCV克隆。