公开(公告)号：EP2256189A1

公开(公告)日：2010-12-01

申请号：EP10174310.2

申请日：2004-03-26

申请人： NATIONAL INSTITUTE OF AGROBIOLOGICAL SCIENCES ,  NIPPON PAPER INDUSTRIES CO., LTD. ,  SANWA KAGAKU KENKYUSHO CO., LTD.

发明人： Sugita, Koichi ,  Kasahara, Saori ,  Ebinuma, Hiroyasu ,  Takaiwa, Humio ,  Jomori, Takahito ,  Hayashi, Yuji ,  Tashita, Akira ,  Kobara, Yukari

IPC分类号： C12N15/00 ,  A01H5/00 ,  C07K14/605

CPC分类号： C12N15/8257 ,  C07K14/605 ,  C12N15/8216

摘要： The present invention provides a method for highly producing a recombinant protein in a plant storage organ and a GLP-1 derivative. The plant storage organ in which the recombinant protein is highly produced is obtained by transformation with the use of a vector which comprises a recombinant protein gene, a cytokinin-related gene, a drug-resistant gene and a removable DNA element, in which the oytohinin-related gene and the drug-resistant gene exist in the positions so that they can behave together with the DNA element, while the recombinant protein to be expressed in the plant storage organ exists in the position so that it would not behave together with the DNA element. The GLP-1 is produced by using the method, and a derivative having been stabilized against enzymatic digestion is further provided.

摘要翻译： 本发明提供了在植物贮藏器官和GLP-1衍生物中高度产生重组蛋白的方法。 通过使用包含重组蛋白基因，细胞分裂素相关基因，耐药性基因和可除去DNA元件的载体转化获得重组蛋白高度产生的植物贮藏器官，其中奥托列宁 相关基因和耐药基因存在于与DNA元件一起表现的位置，而在植物储存器官中表达的重组蛋白存在于与DNA不一致的位置 元件。 通过使用该方法制备GLP-1，进一步提供了稳定的酶消化衍生物。

公开(公告)号：EP0771815A1

公开(公告)日：1997-05-07

申请号：EP96117513.0

申请日：1996-10-31

申请人： SANWA KAGAKU KENKYUSHO CO., LTD.

发明人： Usui, Toshinao ,  Igami, Takao ,  Kakigami, Takuji ,  Hamashima, Hitoshi ,  Jomori, Takahito ,  Tashita, Akira ,  Ishiwatari, Yoshiro ,  Yokochi, Shoji ,  Mitani, Takahiko ,  Suzuki, Yasuo ,  Hasegawa, Akira

IPC分类号： C07H3/06 ,  C07H5/08 ,  C07H11/04 ,  A61K31/70

CPC分类号： A61K31/7024 ,  C07H3/06 ,  C07H5/08 ,  C07H11/04 ,  Y02P20/55

摘要： There are described sulfate or phosphate saccharide derivatives of the Formula
   wherein R 1 is hydrogen atom or a residue of sulfate, phosphate or L-fucose; R 2 , R 3 and R 4 are hydrogen atom or a residue of sulfate or phosphate, respectively; l is an integer of 0 or 1; m is an integer of 0 - 15; and n is an integer of 0 - 21,
or pharmaceutically acceptable salts thereof, a process for the preparation of the derivatives and salts as well as use thereof, as an anti-inflammatory agent.

摘要翻译： 描述了式CHEM的硫酸盐或磷酸酯糖衍生物，其中R 1是氢原子或硫酸盐，磷酸盐或L-岩藻糖的残基; R2，R3和R4分别是氢原子或硫酸盐或磷酸盐的残基; l是0或1的整数; m为0〜15的整数。 和n为0-21的整数，或其药学上可接受的盐，制备衍生物和盐的方法及其用途作为抗炎剂。