公开(公告)号：EP0835323A1

公开(公告)日：1998-04-15

申请号：EP96922498.0

申请日：1996-06-19

申请人： NEW ENGLAND BIOLABS, INC.

发明人： COMB, Donald, G. ,  PERLER, Francine, B. ,  JACK, William, E. ,  XU, Ming-Qun ,  HODGES, Robert, A. ,  NOREN, Christopher, J. ,  CHONG, Shaorong, S., C.

IPC分类号： C12N15 ,  C07K1 ,  C07K14 ,  C07K19 ,  C12N9 ,  C12P21

CPC分类号： C12Y302/01081 ,  C07K1/1075 ,  C07K2319/20 ,  C07K2319/21 ,  C07K2319/92 ,  C07K2319/95 ,  C12N9/1252 ,  C12N9/14 ,  C12N9/22 ,  C12N9/2442 ,  C12N9/2471 ,  C12N15/62 ,  C12P21/02 ,  C12Y302/01014 ,  C12Y302/01023

摘要： The present invention is directed to modified proteins and methods of their production. The modified proteins comprise a controllable intervening protein sequence (CIVPS) inserted into a target protein, the CIVPS being capable of excision from or cleavage of the modified protein under predetermined conditions in cis or in trans, i.e., increase in temperature, exposure to light, unblocking of amino acid residues by dephosphorylation, treatment with chemical reagents or deglycosylation. If desired, the modified protein can be subjected to these conditions. The CIVPS may also be inserted into a region that substantially inactivates target protein activity.