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2.发明公开GRIMONTIA-HOLLISAE-DERIVED RECOMBINANT COLLAGENASE AND ENZYME AGENT FOR CELL SEPARATION 审中-公开REKOMBINANTE COLLAGENASE AUS GRIMONTIA HOLLISAE UND ENZYMMITTEL ZUR ZELLTRENNUNG
公开(公告)号：EP3115455A1
公开(公告)日：2017-01-11
申请号：EP15757886.5
申请日：2015-03-06
发明人： TERAMURA, Naoko , IIJIMA, Katsumasa , HAYASHIDA, Osamu , TANAKA, Keisuke , HATTORI, Shunji , OKITSU, Teru , TAKEUCHI, Shoji
CPC分类号： C12N9/52 , C12Y304/24007
摘要： Recombinant collagenases with a stable specific activity and enzyme agents for cell and tissue dissociation such a recombinant are provided. The recombinant collagenase is derived from Grimontia hollisae -derived collagenase is characterized by having, from the N terminus to the C terminus, a collagenase catalytic domain, a linker region sequence, and a prepeptidase C terminal domain, which Grimontia hollisae -derived recombinant collagenase does not comprise at least the prepeptidase C terminal domain. The obtained recombinant collagenase has a high and stable specific activity.
摘要翻译： 提供具有稳定比活性的重组胶原酶和用于细胞和组织解离的酶剂，如重组体。 重组胶原酶衍生自来自海带石斛的衍生胶原酶，其特征在于从N末端至C末端具有胶原酶催化结构域，接头区序列和前肽酶C末端结构域，其中，Grimontia hollisae衍生的重组胶原酶 不至少包含前肽酶C末端结构域。 获得的重组胶原酶具有高稳定的比活性。
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3.发明公开COLLAGEN POWDER AND/OR COLLAGEN-DERIVED POWDER, AND PRODUCTION METHOD FOR SAME 审中-公开胶原蛋白粉和/或胶原得到的粉末及其制造方法
公开(公告)号：EP2599820A1
公开(公告)日：2013-06-05
申请号：EP11812644.0
申请日：2011-07-29
申请人： NIPPI, INCORPORATED
发明人： TANAKA, Keisuke , OGURA ,Takayuki , HATTORI, Shunji , MATSUDA, Koichi , KOBAYASHI, Yoshikatsu , OI, Shoji
摘要： Disclosed is a collagen powder and/or a collagen derivative powder, which are obtained by dispersing in a hydrophilic organic solvent a crude collagen precipitate which comprises 12 to 50% by mass of a collagen precipitate and/or a collagen derivative precipitate having an average particle size of I to 1,000 µm, recovering solids and then drying the solids. By dispersing the crude collagen precipitate in the hydrophilic organic solvent, the resulting precipitates can be dehydrated, so that drying of the thus obtained solids can be done by air-drying. In addition, the resulting collagen powder and/or collagen derivative powder exhibit excellent solubility due to an increased specific surface area and also have excellent ease of handling with the average particle size being 8 to 1,000 µm.
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公开(公告)号：EP2599820B1
公开(公告)日：2019-12-18
申请号：EP11812644.0
申请日：2011-07-29
申请人： NIPPI, INCORPORATED
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公开(公告)号：EP2803371A1
公开(公告)日：2014-11-19
申请号：EP13735626.7
申请日：2013-01-11
申请人： Nippi Incorporated
IPC分类号： A61L27/00 , A61K8/64 , A61K38/17 , A61P7/04 , A61P17/02 , A61P19/02 , A61P35/00 , A61P37/02 , A61P43/00
摘要： Provided is a collagen structure characterized by: comprising collagen fibers of 1 to 5 µm in average diameter; and has a water content of 0 to 15 (w/w)% and a collagen density of 50 to 800 mg/cm 3 . After generating collagen fibers by neutralizing an acidic collagen solution, the resulting solution is subjected to filtration or the like to form crude collagen fibers having a collagen concentration of 12 to 50 (w/v)%. The thus obtained crude collagen fibers are molded into a prescribed shape and then dried, thereby the collagen structure can be produced. Since the collagen structure is produced using, as raw material, collagen fibers that are formed by association of collagen molecules, the collagen structure has excellent cell infiltration property. Further, since the collagen density of the collagen structure is equivalent to that of in vivo collagen tissue, the collagen structure exhibits excellent tissue regeneration capacity when filled into a defective part in vivo. Therefore, the collagen structure can be preferably used as an artificial material for regenerative medicine and the like.
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