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    METHOD FOR PRODUCING PROTEIN
    2.
    发明公开
    METHOD FOR PRODUCING PROTEIN 审中-公开
    生产蛋白质的方法

    公开(公告)号：EP2990476A9

    公开(公告)日：2016-08-03

    申请号：EP14776222.3

    申请日：2014-03-26

    申请人： NIPPI, INCORPORATED

    发明人： UENO, Tomonori ,  TAGA, Yuki ,  GOTO, Kiyoko ,  KAKU, Yuko

    IPC分类号： C12N5/10 ,  A61K45/00 ,  A61K48/00 ,  A61P11/00 ,  A61P43/00 ,  C12N15/09 ,  C12P21/02

    CPC分类号： C07K14/47 ,  C07K14/70553 ,  C12N15/67 ,  C12N15/85 ,  C12P21/00 ,  C12P21/02

    摘要： It has been believed that promoting the assembly of polysomes composed of many ribosomes attached to mRNA is very effective for highly efficient protein synthesis. However, the mechanism for p180 protein's capability of promoting polysome formation has been yet to be elucidated.
    The inventors of the present application newly discovered SF3b4 protein as a protein that specifically interacts with the coiled-coil domain of p180 protein, a responsible region for its capability of promoting polysome formation, and which is capable of promoting mRNA localization to an endoplasmic reticulum (ER). The inventors also found that, in cells capable of highly expressing both p 180 protein and a protein promoting mRNA localization to an endoplasmic reticulum (ER) (e.g., SF3b4 protein), the mRNA localization to the endoplasmic reticulum can be significantly elevated so that the secretory capacity in cultured cells can be enhanced. Further, the inventors demonstrated that when a particular nucleotide sequence is inserted into an expression plasmid, SF3b4 protein exhibiting protein expression enhancing ability can be localized onto the endoplasmic reticulum membrane, and the mRNA distribution in polysomes can be shifted towards heavier fractions, whereby the secretory capacity in cells can be enhanced.

    摘要翻译： 据认为，促进由连接到mRNA的许多核糖体组成的多核糖体组装对于高效蛋白质合成是非常有效的。 然而，p180蛋白促进多核糖体形成的能力的机制尚未阐明。 本申请的发明人新发现了SF3b4蛋白作为与p180蛋白的卷曲螺旋结构域特异性相互作用的蛋白质，p180蛋白是促进多核糖体形成的能力的责任区域，并且其能够促进mRNA定位至内质网 ER）。 发明人还发现，在能够高度表达p180蛋白和促进内质网（ER）的mRNA定位的蛋白（例如SF3b4蛋白）的细胞中，可以显着提高定位于内质网的mRNA，使得 可以提高培养细胞的分泌能力。 此外，本发明人证实，当将特定核苷酸序列插入表达质粒中时，表现出蛋白表达增强能力的SF3b4蛋白可定位于内质网膜上，并且多核糖体中的mRNA分布可朝向较重的级分移动，由此分泌 细胞容量可以增强。

    METHOD FOR PRODUCING PROTEIN
    3.
    发明公开
    METHOD FOR PRODUCING PROTEIN 审中-公开
    VERFAHREN ZUR PROTEINHERSTELLUNG

    公开(公告)号：EP2990476A1

    公开(公告)日：2016-03-02

    申请号：EP14776222.3

    申请日：2014-03-26

    申请人： NIPPI, INCORPORATED

    发明人： UENO, Tomonori ,  TAGA, Yuki ,  GOTO, Kiyoko ,  KAKU, Yuko

    IPC分类号： C12N5/10 ,  A61K45/00 ,  A61K48/00 ,  A61P11/00 ,  A61P43/00 ,  C12N15/09 ,  C12P21/02

    CPC分类号： C07K14/47 ,  C07K14/70553 ,  C12N15/67 ,  C12N15/85 ,  C12P21/00 ,  C12P21/02

    摘要： It has been believed that promoting the assembly of polysomes composed of many ribosomes attached to mRNA is very effective for highly efficient protein synthesis. However, the mechanism for p180 protein's capability of promoting polysome formation has been yet to be elucidated.
    The inventors of the present application newly discovered SF3b4 protein as a protein that specifically interacts with the coiled-coil domain of p180 protein, a responsible region for its capability of promoting polysome formation, and which is capable of promoting mRNA localization to an endoplasmic reticulum (ER). The inventors also found that, in cells capable of highly expressing both p 180 protein and a protein promoting mRNA localization to an endoplasmic reticulum (ER) (e.g., SF3b4 protein), the mRNA localization to the endoplasmic reticulum can be significantly elevated so that the secretory capacity in cultured cells can be enhanced. Further, the inventors demonstrated that when a particular nucleotide sequence is inserted into an expression plasmid, SF3b4 protein exhibiting protein expression enhancing ability can be localized onto the endoplasmic reticulum membrane, and the mRNA distribution in polysomes can be shifted towards heavier fractions, whereby the secretory capacity in cells can be enhanced.

    摘要翻译： 据认为，促进由连接到mRNA的许多核糖体组成的多核糖体的组装对于高效的蛋白质合成是非常有效的。 然而，p180蛋白促进多形核糖体形成的能力的机制尚未阐明。 本申请的发明人新发现SF3b4蛋白作为与p180蛋白的卷曲螺旋结构域特异性相互作用的蛋白质，其是促进多聚体形成能力的负责区域，并且能够促进mRNA定位于内质网（ ER）。 本发明人还发现，在能够高度表达p 180蛋白和蛋白质促进mRNA定位于内质网（ER）（例如，SF3b4蛋白）的细胞中）的细胞中，可以显着提高对内质网的mRNA定位， 培养细胞的分泌能力可以提高。 此外，本发明人发现当将特定的核苷酸序列插入表达质粒时，表现出蛋白表达增强能力的SF3b4蛋白可以定位在内质网膜上，并且多核糖体中的mRNA分布可以转移到更重的部分，由此分泌 可以增强细胞的能力。

    GRIMONTIA-HOLLISAE-DERIVED RECOMBINANT COLLAGENASE AND ENZYME AGENT FOR CELL SEPARATION
    4.
    发明公开
    GRIMONTIA-HOLLISAE-DERIVED RECOMBINANT COLLAGENASE AND ENZYME AGENT FOR CELL SEPARATION 审中-公开
    REKOMBINANTE COLLAGENASE AUS GRIMONTIA HOLLISAE UND ENZYMMITTEL ZUR ZELLTRENNUNG

    公开(公告)号：EP3115455A4

    公开(公告)日：2017-09-13

    申请号：EP15757886

    申请日：2015-03-06

    申请人： NIPPI INCORPORATED ,  UNIV TOKYO

    发明人： TERAMURA NAOKO ,  IIJIMA KATSUMASA ,  HAYASHIDA OSAMU ,  TANAKA KEISUKE ,  HATTORI SHUNJI ,  OKITSU TERU ,  TAKEUCHI SHOJI

    IPC分类号： C12N15/09 ,  C12N9/52

    CPC分类号： C12N9/52 ,  C12Y304/24007

    摘要： Recombinant collagenases with a stable specific activity and enzyme agents for cell and tissue dissociation such a recombinant are provided. The recombinant collagenase is derived from Grimontia hollisae -derived collagenase is characterized by having, from the N terminus to the C terminus, a collagenase catalytic domain, a linker region sequence, and a prepeptidase C terminal domain, which Grimontia hollisae -derived recombinant collagenase does not comprise at least the prepeptidase C terminal domain. The obtained recombinant collagenase has a high and stable specific activity.

    摘要翻译： 提供具有稳定比活性的重组胶原酶和用于细胞和组织解离的酶试剂，例如重组体。 重组胶原酶源自Grimontia hollisae衍生的胶原酶，其特征在于从N末端至C末端具有胶原酶催化结构域，接头区序列和前肽酶C末端结构域，其中来自Grimontia hollisae的重组胶原酶 不包含至少前肽酶C末端结构域。 获得的重组胶原酶具有高且稳定的比活性。

    GRIMONTIA-HOLLISAE-DERIVED RECOMBINANT COLLAGENASE AND ENZYME AGENT FOR CELL SEPARATION
    5.
    发明公开
    GRIMONTIA-HOLLISAE-DERIVED RECOMBINANT COLLAGENASE AND ENZYME AGENT FOR CELL SEPARATION 审中-公开
    REKOMBINANTE COLLAGENASE AUS GRIMONTIA HOLLISAE UND ENZYMMITTEL ZUR ZELLTRENNUNG

    公开(公告)号：EP3115455A1

    公开(公告)日：2017-01-11

    申请号：EP15757886.5

    申请日：2015-03-06

    申请人： NIPPI, INCORPORATED ,  THE UNIVERSITY OF TOKYO

    发明人： TERAMURA, Naoko ,  IIJIMA, Katsumasa ,  HAYASHIDA, Osamu ,  TANAKA, Keisuke ,  HATTORI, Shunji ,  OKITSU, Teru ,  TAKEUCHI, Shoji

    IPC分类号： C12N15/09 ,  C12N9/52

    CPC分类号： C12N9/52 ,  C12Y304/24007

    摘要： Recombinant collagenases with a stable specific activity and enzyme agents for cell and tissue dissociation such a recombinant are provided. The recombinant collagenase is derived from Grimontia hollisae -derived collagenase is characterized by having, from the N terminus to the C terminus, a collagenase catalytic domain, a linker region sequence, and a prepeptidase C terminal domain, which Grimontia hollisae -derived recombinant collagenase does not comprise at least the prepeptidase C terminal domain. The obtained recombinant collagenase has a high and stable specific activity.

    摘要翻译： 提供具有稳定比活性的重组胶原酶和用于细胞和组织解离的酶剂，如重组体。 重组胶原酶衍生自来自海带石斛的衍生胶原酶，其特征在于从N末端至C末端具有胶原酶催化结构域，接头区序列和前肽酶C末端结构域，其中，Grimontia hollisae衍生的重组胶原酶 不至少包含前肽酶C末端结构域。 获得的重组胶原酶具有高稳定的比活性。

    COLLAGEN POWDER AND/OR COLLAGEN-DERIVED POWDER, AND PRODUCTION METHOD FOR SAME
    6.
    发明公开
    COLLAGEN POWDER AND/OR COLLAGEN-DERIVED POWDER, AND PRODUCTION METHOD FOR SAME 审中-公开
    胶原蛋白粉和/或胶原得到的粉末及其制造方法

    公开(公告)号：EP2599820A1

    公开(公告)日：2013-06-05

    申请号：EP11812644.0

    申请日：2011-07-29

    申请人： NIPPI, INCORPORATED

    发明人： TANAKA, Keisuke ,  OGURA ,Takayuki ,  HATTORI, Shunji ,  MATSUDA, Koichi ,  KOBAYASHI, Yoshikatsu ,  OI, Shoji

    IPC分类号： C08H1/00 ,  A61K8/65 ,  A61K38/00 ,  C08J3/14 ,  C08L89/00

    摘要： Disclosed is a collagen powder and/or a collagen derivative powder, which are obtained by dispersing in a hydrophilic organic solvent a crude collagen precipitate which comprises 12 to 50% by mass of a collagen precipitate and/or a collagen derivative precipitate having an average particle size of I to 1,000 µm, recovering solids and then drying the solids. By dispersing the crude collagen precipitate in the hydrophilic organic solvent, the resulting precipitates can be dehydrated, so that drying of the thus obtained solids can be done by air-drying. In addition, the resulting collagen powder and/or collagen derivative powder exhibit excellent solubility due to an increased specific surface area and also have excellent ease of handling with the average particle size being 8 to 1,000 µm.

    Unit for grinding sample, unit for grinding and collecting sample, and process for grinding sample
    7.
    发明公开
    Unit for grinding sample, unit for grinding and collecting sample, and process for grinding sample 审中-公开
    单元，其用于研磨的样品单元，其用于磨削和收集样品，并且过程进行研磨样品

    公开(公告)号：EP2529837A2

    公开(公告)日：2012-12-05

    申请号：EP12004133.0

    申请日：2012-05-29

    申请人： NIPPI, INCORPORATED

    发明人： Yamamoto, Takuji ,  Higa, Kiyonori ,  Hattori, Shunji

    IPC分类号： B02C19/08

    CPC分类号： B02C19/08 ,  A61J7/0007 ,  B02C23/16 ,  G01N1/286 ,  G01N2001/2866 ,  Y10S241/27

    摘要： Provided is a unit which is capable of readily grinding and collecting a small amount of a sample which is difficult to be ground. The unit for grinding a sample comprises a club-shaped pestle and a mortar into which the pestle can be inserted, wherein the pestle comprises a roughened taper located at at least one end of its pestle body, wherein the mortar is a tube with an inverted frustoconical bottom, and the surface of the mortar where the taper of the pestle contacts the inverted frustoconical bottom when the pestle is inserted into the mortar is roughened, and wherein the angle of the taper with respect to the longitudinal centerline of the pestle is substantially equal to the angle of the inner wall of the inverted frustoconical bottom with respect to the longitudinal centerline of the mortar. The roughened taper and surface improve grinding efficiency.

    摘要翻译： 本发明提供一种单元的所有其能够容易地研磨和收集样品的所有这是难以被研磨的量小。 用于研磨样品的单元包括棒状杵和其中杵可插入砂浆，worin杵在倒置包括位于其杵主体的至少一个端部的粗糙锥度，worin砂浆是一个管 截头圆锥形底部和砂浆，其中当杵插入砂浆杵接触倒截头圆锥体底部的锥度被粗糙化，并相对于该杵的纵向中心线worin锥的角的表面基本上是相等 于倒圆锥台底部的内壁的相对于所述砂浆的纵向中心线的角度。 粗糙化表面锥度和提高研磨效率。

    METHOD FOR PRODUCING PROTEIN
    9.
    发明公开
    METHOD FOR PRODUCING PROTEIN 审中-公开

    公开(公告)号：EP3981877A1

    公开(公告)日：2022-04-13

    申请号：EP21196008.3

    申请日：2014-03-26

    申请人： NIPPI, INCORPORATED

    发明人： UENO, Tomonori ,  TAGA, Yuki ,  GOTO, Kiyoko ,  KAKU, Yuko

    IPC分类号： C12N15/113 ,  C12N5/10 ,  A61K45/00 ,  A61K48/00 ,  A61P11/00 ,  A61P43/00 ,  C12N15/09 ,  C12P21/02

    摘要： It has been believed that promoting the assembly of polysomes composed of many ribosomes attached to mRNA is very effective for highly efficient protein synthesis. However, the mechanism for p 180 protein's capability of promoting polysome formation has been yet to be elucidated.
    The inventors of the present application newly discovered SF3b4 protein as a protein that specifically interacts with the coiled-coil domain of p 180 protein, a responsible region for its capability of promoting polysome formation, and which is capable of promoting mRNA localization to an endoplasmic reticulum (ER). The inventors also found that, in cells capable of highly expressing both p180 protein and a protein promoting mRNA localization to an endoplasmic reticulum (ER) (e.g., SF3b4 protein), the mRNA localization to the endoplasmic reticulum can be significantly elevated so that the secretory capacity in cultured cells can be enhanced. Further, the inventors demonstrated that when a particular nucleotide sequence is inserted into an expression plasmid, SF3b4 protein exhibiting protein expression enhancing ability can be localized onto the endoplasmic reticulum membrane, and the mRNA distribution in polysomes can be shifted towards heavier fractions, whereby the secretory capacity in cells can be enhanced.