公开(公告)号：EP0759073A1

公开(公告)日：1997-02-26

申请号：EP95918549.0

申请日：1995-05-11

申请人： NOVO NORDISK A/S

发明人： KOFOD, Lene, Venke ,  ANDERSEN, Lene, Nonboe ,  KAUPPINEN, Markus, Sakari ,  CHRISTGAU, Stephan ,  DALBOGE, Henrik ,  OLSEN, Hans, Sejr ,  BREINHOLT, Jens

IPC分类号： C12N15 ,  A21D8 ,  A61K8 ,  A61Q11 ,  C11D3 ,  C12N1 ,  C12N9 ,  C12P21 ,  D06M16

CPC分类号： C12Y302/01006 ,  A21D8/042 ,  A61K8/66 ,  A61Q11/00 ,  C11D3/38645 ,  C12N9/244 ,  D06M16/003

摘要： The present invention relates to an enzyme with endo-beta-glucanase activity, encoded by a DNA sequence. Further, the invention relates to a DNA construct encoding the enzyme, a method of producing the enzyme, an enzyme preparation containing the enzyme, the use of said enzyme or said preparation for a number of uses including the degradation or modification of beta-glucan containing materials. Also contemplated is an isolated substantially pure culture of Saccharomyces cerevisiae NN049006, transformed with a DNA sequence derived from Acremonium sp.

公开(公告)号：EP0882131A1

公开(公告)日：1998-12-09

申请号：EP97905653.0

申请日：1997-02-07

申请人： NOVO NORDISK BIOTECH, INC. ,  NOVO NORDISK A/S

发明人： BERKA, Randy, M. ,  CHRISTGAU, Stephan ,  SHUSTER, Jeff ,  HALKIER, Torben ,  FUGLSANG, Claus, Crone

IPC分类号： C12N15 ,  C07K19 ,  C12N1 ,  C12N9 ,  C12R1

CPC分类号： C12Y302/01084 ,  C12N9/2402 ,  Y10S435/933 ,  Y10S435/935

摘要： The present invention relates to polypeptides having mutanase activity and isolated nucleic acid sequences encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid sequences as well as methods for producing the polypeptides. The present invention further relates to oral cavity compositions and methods for degrading mutan.

公开(公告)号：EP0710282A1

公开(公告)日：1996-05-08

申请号：EP94919576.0

申请日：1994-07-05

申请人： NOVO NORDISK A/S

发明人： DALB0GE, Henrik ,  CHRISTGAU, Stephan ,  ANDERSEN, Lene Nonboe ,  KOFOD, Lene Venke ,  KAUPPINEN, Markus Sakari

IPC分类号： C12N9 ,  C12N15

CPC分类号： C12N9/2437 ,  C12Y302/01004

摘要： A DNA construct comprising a DNA sequence encoding an enzyme with endoglucanase activity, which DNA sequence comprises the DNA sequence shown in SEQ ID No. 1 or an analogous sequence thereof being at least 70 % homologous to the DNA sequence shown in SEQ ID No. 1. The DNA sequence may be isolated from a strain of Trichoderma harzianum. The endoglucanase expressed from the DNA construct may be used for the degradation or modification of plant materials.

公开(公告)号：EP0707641A1

公开(公告)日：1996-04-24

申请号：EP94919575.0

申请日：1994-07-05

申请人： NOVO NORDISK A/S

发明人： DALBOGE, Henrik ,  CHRISTGAU, Stephan ,  ANDERSEN, Lene Nonboe ,  KOFOD, Lene Venke ,  KAUPPINEN, Markus Sakari ,  NIELSEN, Jack Bech ,  DAMBMANN, Claus

IPC分类号： C12N1 ,  A21D2 ,  A21D8 ,  A23K1 ,  C11D3 ,  C12N5 ,  C12N9 ,  C12N15 ,  C12P21 ,  C12R1

CPC分类号： C11D3/3947 ,  A21D8/042 ,  A23K20/189 ,  C11D3/0078 ,  C11D3/386 ,  C11D3/38618 ,  C11D3/38636 ,  C12N9/62 ,  C12N9/82

摘要： A DNA construct comprising a DNA sequence encoding an enzyme exhibiting protease activity, which DNA sequence comprises the DNA sequence shown in SEQ ID No. 1 or 2 or an analogue of any of these sequences being at least 80 % homologous to the DNA sequence shown in SEQ ID No. 1 or 2. The proteases encoded by the DNA sequences have an acid pH optimum.

公开(公告)号：EP0702718A1

公开(公告)日：1996-03-27

申请号：EP94914351.0

申请日：1994-04-28

申请人： NOVO NORDISK A/S

发明人： CHRISTGAU, Stephan ,  KOFOD, Lene Venke ,  ANDERSEN, Lene Nonboe ,  KAUPPINEN, Sakari ,  HELDT-HANSEN, Hans Peter ,  DALBOEGE, Henrik

IPC分类号： A23L2 ,  C09K8 ,  C12N9 ,  C12N15

CPC分类号： C09K8/905 ,  A23L2/80 ,  C12N9/2488 ,  C12Y302/01025 ,  C12Y302/01078

摘要： An enzyme exhibiting mannanase activity, which enzyme i) is immunologically reactive with an antibody raised against a purified mannanase derived from Aspergillus aculeatus, CBS 101.43; ii) is encoded by the DNA sequence shown in SEQ ID No. 1 or an analogue of said sequence, and/or; iii) comprises the amino acid sequence shown in SEQ ID No. 2 or a sequence being at least 70 % homologous thereto. The enzyme may be used for various purposes for which degradation or modification of a plant or algal cell wall material is desirable.

公开(公告)号：EP0687298A1

公开(公告)日：1995-12-20

申请号：EP94908993.0

申请日：1994-03-04

申请人： NOVO NORDISK A/S

发明人： KOFOD, Lene Venke ,  DALBOGE, Henrik ,  ANDERSEN, Lene Nonboe ,  KAUPPINEN, Markus Sakari ,  CHRISTGAU, Stephan ,  HELDT-HANSEN, Hans Peter ,  CHRISTOPHERSEN, Claus ,  NIELSEN, Per Munk ,  VORAGEN, Alphons Gerard Joseph ,  SCHOLS, Hendrik Arie

IPC分类号： A23K1 ,  A23L1 ,  A23L2 ,  C12N9 ,  C12N15 ,  C13B10 ,  C13K13 ,  C12R1

CPC分类号： C13B10/083 ,  A23K20/189 ,  A23L2/84 ,  A23L29/06 ,  C12N9/2402 ,  C12Y302/01171 ,  C13K13/00

摘要： An enzyme exhibiting rhamnogalacturonase activity, which enzyme: a) is encoded by the DNA sequence shown in SEQ ID No. 1 or a sequence homologous thereto encoding a polypeptide with RGase activity, b) has the amino acid sequence shown in SEQ ID No. 2 or an analogue thereof, c) is reactive with an antibody raised against the enzyme encoded by the DNA sequence shown in SEQ ID No. 1, d) has a pH optimum above pH 5, and/or e) has a relative activity of at least 30% at a pH in the range of 5.5-6.5. The enzyme may be used for reducing the viscosity of a plant cell wall material and for extracting high molecular weight molecules from a plant cell wall material.

摘要翻译： 显示鼠李糖半乳糖醛酸酶活性的酶，其中酶：a）由SEQ ID No.1所示的DNA序列或与其同源的编码具有RGase活性的多肽的序列编码，b）具有SEQ ID No.2所示的氨基酸序列 c）与针对由SEQ ID No.1所示的DNA序列编码的酶产生的抗体反应，d）具有高于pH 5的最佳pH，和/或e）具有以下的相对活性： 在5.5-6.5范围内的pH下至少30％。 该酶可用于降低植物细胞壁材料的粘度并从植物细胞壁材料中提取高分子量分子。

公开(公告)号：EP0759977A1

公开(公告)日：1997-03-05

申请号：EP95918550.0

申请日：1995-05-11

申请人： NOVO NORDISK A/S

发明人： KOFOD, Lene Venke ,  ANDERSEN, Lene Nonboe ,  KAUPPINEN, Markus Sakari ,  CHRISTGAU, Stephan ,  DALBOGE, Henrik ,  OLSEN, Hans Sejr

IPC分类号： C12G1 ,  A23L2 ,  C12N1 ,  C12N9 ,  C12N15 ,  C12P21 ,  C12R1

CPC分类号： C12Y302/01075 ,  C12N9/2402 ,  Y10S435/945

摘要： The present invention relates to an enzyme with endo-beta-glucanase activity, encoded by a DNA sequence. Further, the invention relates to a DNA construct encoding the enzyme, a method of producing the enzyme, an enzyme preparation containing the enzyme, the use of said enzyme or said preparation for a number of uses including the degradation or modification of beta-glucan containing materials.

摘要翻译： 本发明涉及由DNA序列编码的具有内切-β-葡聚糖酶活性的酶。 此外，本发明涉及编码该酶的DNA构建体，该酶的生产方法，含酶的酶制剂，所述酶或所述制剂用于多种用途，包括降解或修饰含β-葡聚糖的 材料。

公开(公告)号：EP0695349A1

公开(公告)日：1996-02-07

申请号：EP94908986.0

申请日：1994-03-02

申请人： NOVO NORDISK A/S

发明人： KOFOD, Lene Venke ,  KAUPPINEN, Markus Sakari ,  CHRISTGAU, Stephan ,  HELDT-HANSEN, Hans Peter ,  DALBOGE, Henrik ,  ANDERSEN, Lene Nonboe ,  SI, Joan Qi ,  JACOBSEN, Tina Sejersgärd ,  MUNK, Niels ,  MÜLLERTZ, Anette

IPC分类号： A21D8 ,  A23K1 ,  A23L1 ,  A23L2 ,  C12C5 ,  C12C7 ,  C12G1 ,  C12N9 ,  C12N15 ,  D21C5

CPC分类号： C12Y302/01032 ,  A21D8/042 ,  A23K20/189 ,  A23K50/75 ,  A23L2/04 ,  A23L7/107 ,  A23L29/06 ,  C12C5/004 ,  C12C5/006 ,  C12C7/28 ,  C12G1/00 ,  C12G1/0203 ,  C12N9/2482 ,  C12Y302/01008 ,  D21C5/005

摘要： An enzyme exhibiting xylanase activity, which enzyme is immunologically reactive with an antibody raised against a purified xylanase derived from Aspergillus aculeatus, CBS 101.43. The enzyme may be used for degrading plant cell wall components e.g. in the preparation of feed, in baking, in the paper and pulp industry and in connection with separation of wheat into starch and gluten.

公开(公告)号：EP0710282B1

公开(公告)日：1999-03-03

申请号：EP94919576.2

申请日：1994-07-05

申请人： NOVO NORDISK A/S

发明人： DALB0GE, Henrik ,  CHRISTGAU, Stephan ,  ANDERSEN, Lene Nonboe ,  KOFOD, Lene Venke ,  KAUPPINEN, Markus Sakari

IPC分类号： C12N9/42

CPC分类号： C12N9/2437 ,  C12Y302/01004

摘要： A DNA construct comprising a DNA sequence encoding an enzyme with endoglucanase activity, which DNA sequence comprises the DNA sequence shown in SEQ ID No. 1 or an analogous sequence thereof being at least 70 % homologous to the DNA sequence shown in SEQ ID No. 1. The DNA sequence may be isolated from a strain of Trichoderma harzianum. The endoglucanase expressed from the DNA construct may be used for the degradation or modification of plant materials.

公开(公告)号：EP0675951A1

公开(公告)日：1995-10-11

申请号：EP94903748.0

申请日：1993-12-23

申请人： NOVO NORDISK A/S

发明人： DALBOEGE, Henrik ,  ANDERSEN, Lene, Nonboe ,  KOFOD, Lene, Venke ,  KAUPPINEN, Markus, Sakari ,  CHRISTGAU, Stephan

IPC分类号： C12N9 ,  C12N15

CPC分类号： C12N9/2437 ,  C12Y302/01004

摘要： An enzyme exhibiting endoglucanase activity, which enzyme is encoded by a DNA sequence comprising at least one of the following partial sequences: (a) ATTCATTTGTG GACAGTGGAC, (b) GTTGATCGCA CATTGAACCA, (c) ACCCCAGCCG ACCGATTGTC, (d) CTTCCTTACC TCACCATCAT, (e) TTAACATCTT TTCACCATGA, (f) AGCTTTCCCT TCTCTCCCTT, (g) GCCACCCTGG CTTCCGCTGC CAGCCTCC, (h) GACAGTAGCA ATCCAGCATT, (i) AGCATCAGCC GCTTTGTACA, (j) CCATGAAGTT CACCGTATTG, (k) GCACTGCTTC TCTCCCAGGT, (l) GTGGGCGGCC CCTCAGGCAA, (m) ACGCTCCTCC AATTTTCTCT, (n) GGCTTGGTAG TAATGAGTCT, (o) GGCGCAGAGT TTGGCCAGGC, (p) CAACATCCCC GGTGTTCTGGG. The enzyme may be produced by recombinant DNA techniques and may be used for degradation of plant cell wall material.