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公开(公告)号：EP3510171A1
公开(公告)日：2019-07-17
申请号：EP17821368.2
申请日：2017-06-30
申请人： Natera, Inc.
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公开(公告)号：EP3792365A1
公开(公告)日：2021-03-17
申请号：EP20203880.8
申请日：2017-06-30
申请人： Natera, Inc.
发明人： ZIMMERMAN, Bernhard , BABIARZ, Joshua , SALARI, Raheleh , CONSTANTIN, Tudor Pompiliu , SAKARYA, Onur , PROSEN, Dennis , DASHMER, Scott , SERGEEV, Nikolay , HILL, Matthew Micah
IPC分类号： C12Q1/68 , C12Q1/6827
摘要： The invention provides methods and compositions for detecting a mutation in a target gene in a sample or a fraction thereof, including in certain examples, a fraction that includes circulating tumor DNA. The methods can include a tiling PCR reaction, for example a one-sided multiplex tiling reaction. Virtually any type of mutation can be detected with the methods and compositions. In certain embodiments, gene fusions are detected. Improved PCR methods, especially for performing nested multiplex PCR reactions are provided.
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公开(公告)号：EP2885445A1
公开(公告)日：2015-06-24
申请号：EP13829596.9
申请日：2013-08-15
申请人： Natera, Inc.
CPC分类号： C12Q1/6855 , C12N15/1093 , C12Q2535/122 , C12Q2525/186 , C12Q2525/191
摘要： Embodiments include methods, compositions, and kits for creating genetic libraries useful for massively parallel genetic sequencing. Some embodiments are directed to methods of preventing the contamination of genetic libraries with material generated during the formation of other genetic libraries. In some embodiments, the methods employ adapters comprising universal priming sites. The methods can employ non-ligatable primers to generate non-ligatable amplification products so as to prevent unwanted ligation to adapters. In some embodiments, the non-ligatable primers contain uracil. Genetic material can be treated with uracil N glycosylase to prevent the unwanted ligation of uracil containing amplicons to adapters used for creating a second genetic library.
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