公开(公告)号：EP3311660A1

公开(公告)日：2018-04-25

申请号：EP16814302.2

申请日：2016-06-20

申请人： National Federation of Agricultural Cooperative Associations ,  The University Of Tokyo

发明人： FUKAWA, Kazuo ,  ITO, Tetsuya ,  KAMIKAWA, Mai ,  SENDAI, Yutaka ,  NAKAUCHI, Hiromitsu ,  YAMAZAKI, Satoshi ,  WATANABE, Motoo

IPC分类号： A01K67/027 ,  C12N5/16 ,  C12N15/09

CPC分类号： A01K67/0271 ,  A01K67/027 ,  A01K2207/12 ,  A01K2217/052 ,  A01K2227/108 ,  A01K2267/025 ,  C12N5/0647 ,  C12N15/09 ,  C12N2510/00 ,  C12N2510/02 ,  C12N2517/02

摘要： The present invention discloses a novel means capable of producing a blood chimeric animal in which a state of retaining blood cells originating in a heterologous animal, such as a human, at a high percentage is sustained for a long period of time, even in cases where a medium to large domestic animal is used as a recipient. The method for producing a non-human animal that retains blood cells originating in a heterologous animal, according to the present invention, comprises transplanting hematopoietic cells of a heterologous animal into a non-human animal, in which hematopoietic cells the function of a gene that acts on the hematopoietic system is modified. The gene that acts on the hematopoietic system is, for example, Lnk gene. According to the method of the present invention, when a medium to large mammal such as a pig is used as a recipient, the survival rate of hematopoietic cells originating in a heterologous animal is dramatically increased such that blood chimerism of 10% or more can be maintained even in a 16 month old animal.

摘要翻译： 本发明公开了一种能够制造血液嵌合体动物的新型方法，其中以高百分比保存源自异源动物如人的血细胞的状态持续很长一段时间，即使在 一个大型的家养动物被用作接受者。 根据本发明的用于生产保留源自异源动物的血细胞的非人动物的方法包括将异源动物的造血细胞移植到非人动物中，其中造血细胞具有基因的功能， 对造血系统的作用被修改。 作用于造血系统的基因例如是Lnk基因。 根据本发明的方法，当使用猪等大型哺乳动物作为受体时，源自异源动物的造血细胞的存活率显着增加，使得10％或更多的血液嵌合体可以是 即使在16个月大的动物中也能保持。