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1.发明公开METHOD FOR SEPARATING CELLS, CELL CULTURE MEDIUM, AND DEVICE FOR SEPARATING CELLS 审中-公开方法用于分离细胞的分离细胞中，细胞培养基和设备
公开(公告)号：EP2554657A1
公开(公告)日：2013-02-06
申请号：EP11765515.9
申请日：2011-03-28
发明人： SUMARU Kimio , TAKAGI Toshiyuki , KIKUCHI Kyoko , SATO Taku , YAMAGUCHI Manae , KANAMORI Toshiyuki
摘要： A method for separating cells which includes: adhering cells to the surface of a cell culture substrate containing a photo-acid generator that generates an acidic substance upon irradiation with active energy rays, and irradiating only a partial region of the cell culture substrate with the active energy rays to selectively remove the cells within the partial region, thereby separating the cells within the partial region and cells in other regions.
摘要翻译： 一种用于细胞，其包括分离方法：粘附的细胞，以含有光酸产生剂的细胞培养基材的表面做了在用活性能量射线的照射生成的酸性物质的速率，并与活性仅照射的细胞培养基材的部分区域 能量射线选择性地去除所述部分区域内的细胞，从而分离所述部分区域内的细胞在其他区域中的细胞和。
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公开(公告)号：EP3000836B1
公开(公告)日：2018-12-26
申请号：EP14801820.3
申请日：2014-05-13
CPC分类号： C08G65/33389 , C08G65/333 , C08G65/33337 , C08G69/40 , C08G81/00 , C12M23/20 , C12M23/30 , C12N5/0068 , C12N2537/10
摘要： The present invention provides a photodegradable cross-linking agent capable of manufacturing a photodegradable gel, which has appropriate moisture content and water solubility as a cell carrier and has strength that makes it possible to construct a complicated three-dimensional microstructure. The photodegradable cross-linking agent of the present invention includes a main chain 2 which is composed of branched polyethylene glycol having three or more branched chains and a photodegradable benzyl group 3 which is disposed on the terminus of the branched chains, in which the benzyl group has an active ester group 4, which is reactive with an amino group or a hydroxyl group, and one or more nitro groups in a benzene ring.
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公开(公告)号：EP2554657B1
公开(公告)日：2019-02-20
申请号：EP11765515.9
申请日：2011-03-28
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4.发明公开PHOTODEGRADABLE CROSSLINKING AGENT, PHOTODEGRADABLE GEL, CELL CULTURE DEVICE, CELL ARRANGEMENT/SEPARATION DEVICE, CELL ARRANGEMENT METHOD, CELL SEPARATION METHOD, METHOD OF FORMING TISSUE MATERIAL, AND TISSUE MATERIAL 审中-公开光降解网络资源，光降解GEL，细胞培养装置，ZELLANORDNUNGS- /分离器，ZELLA订购方法，细胞分离，产生的织物材料与组织材料的方法
公开(公告)号：EP3000836A1
公开(公告)日：2016-03-30
申请号：EP14801820.3
申请日：2014-05-13
CPC分类号： C08G65/33389 , C08G65/333 , C08G65/33337 , C08G69/40 , C08G81/00 , C12M23/20 , C12M23/30 , C12N5/0068 , C12N2537/10
摘要： The present invention provides a photodegradable cross-linking agent capable of manufacturing a photodegradable gel, which has appropriate moisture content and water solubility as a cell carrier and has strength that makes it possible to construct a complicated three-dimensional microstructure. The photodegradable cross-linking agent of the present invention includes a main chain 2 which is composed of branched polyethylene glycol having three or more branched chains and a photodegradable benzyl group 3 which is disposed on the terminus of the branched chains, in which the benzyl group has an active ester group 4, which is reactive with an amino group or a hydroxyl group, and one or more nitro groups in a benzene ring.
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