公开(公告)号：EP2757376A9

公开(公告)日：2014-11-26

申请号：EP12831729.4

申请日：2012-09-14

申请人： National University Corporation Nagoya University ,  Medical and Biological Laboratories Co., Ltd. ,  Oncomics Co., Ltd.

发明人： YANAGISAWA, Kiyoshi ,  TAKAHASHI, Takashi ,  YOKOI, Kohei ,  HASEGAWA, Yoshinori ,  ONO, Ken-ichiro ,  YAGI, Kasumi ,  MASUDA, Hitomi ,  TAKEUCHI, Toshiyuki

IPC分类号： G01N33/574 ,  C07K14/47 ,  C07K16/18 ,  C12N15/09

CPC分类号： G01N33/57423 ,  C07K14/47 ,  C07K16/18 ,  G01N33/57407

摘要： To develop a simple, low-cost and highly reliable testing method for mesothelioma, and a kit used for said testing method.
The present invention provides a testing method for mesothelioma comprising a step in which the concentration of human periostin protein is measured in at least one type of sample from among samples of the blood or pleural fluid of a subject. The step in which the concentration of human periostin protein is measured may use an antibody directed against human periostin protein. The present invention further provides a kit for diagnosing mesothelioma, said kit including the antibody directed against human periostin protein. In one kit for diagnosing mesothelioma, the diagnosis of mesothelioma determines if a subject that may have mesothelioma has mesothelioma or is healthy. In another kit for diagnosing mesothelioma, the diagnosis of mesothelioma determines if a subject that may have mesothelioma has mesothelioma or has a respiratory illness other than mesothelioma.

公开(公告)号：EP2757376A1

公开(公告)日：2014-07-23

申请号：EP12831729.4

申请日：2012-09-14

申请人： National University Corporation Nagoya University ,  Medical and Biological Laboratories Co., Ltd. ,  Oncomics Co., Ltd.

发明人： YANAGISAWA, Kiyoshi ,  TAKAHASHI, Takashi ,  YOKOI, Kohei ,  HASEGAWA, Yoshinori ,  ONO, Ken-ichiro ,  YAGI, Kasumi ,  MASUDA, Hitomi ,  TAKEUCHI, Toshiyuki

IPC分类号： G01N33/574 ,  C07K14/47 ,  C07K16/18 ,  C12N15/09

CPC分类号： G01N33/57423 ,  C07K14/47 ,  C07K16/18 ,  G01N33/57407

摘要： To develop a simple, low-cost and highly reliable testing method for mesothelioma, and a kit used for said testing method.
The present invention provides a testing method for mesothelioma comprising a step in which the concentration of human periostin protein is measured in at least one type of sample from among samples of the blood or pleural fluid of a subject. The step in which the concentration of human periostin protein is measured may use an antibody directed against human periostin protein. The present invention further provides a kit for diagnosing mesothelioma, said kit including the antibody directed against human periostin protein. In one kit for diagnosing mesothelioma, the diagnosis of mesothelioma determines if a subject that may have mesothelioma has mesothelioma or is healthy. In another kit for diagnosing mesothelioma, the diagnosis of mesothelioma determines if a subject that may have mesothelioma has mesothelioma or has a respiratory illness other than mesothelioma.

摘要翻译： 开发一种简单，低成本，高可靠性的间皮瘤检测方法，以及用于所述检测方法的试剂盒。 本发明提供了一种间皮瘤的检测方法，其特征在于，在受试者的血液或胸膜液样品中的至少一种样品中测定人骨膜素蛋白的浓度。 测量人骨膜素蛋白浓度的步骤可以使用针对人骨膜素蛋白的抗体。 本发明还提供了用于诊断间皮瘤的试剂盒，所述试剂盒包括针对人骨膜素蛋白的抗体。 在诊断间皮瘤的一个试剂盒中，间皮瘤的诊断决定了可能有间皮瘤的受试者是否具有间皮瘤或健康。 在诊断间皮瘤的另一个试剂盒中，间皮瘤的诊断决定了可能有间皮瘤的受试者是否具有间皮瘤或具有间皮瘤以外的呼吸系统疾病。

公开(公告)号：EP2881467B1

公开(公告)日：2018-10-31

申请号：EP13825402.4

申请日：2013-07-30

申请人： National University Corporation Nagoya University ,  Medical & Biological Laboratories Co., Ltd.

发明人： KADOMATSU, Kenji ,  KISHIDA, Satoshi ,  ONO, Kenichiro ,  YAGI, Kasumi

IPC分类号： C12N15/09 ,  A61K39/395 ,  A61P35/00 ,  C07K16/18 ,  C07K16/46 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12P21/08 ,  C07K16/22

CPC分类号： C07K16/22 ,  C07K2317/24 ,  C07K2317/34 ,  C07K2317/55 ,  C07K2317/565 ,  C07K2317/73 ,  C07K2317/76 ,  C07K2317/90

摘要： A monoclonal antibody, which recognizes at least two amino acids among amino acids located at position 69, position 79, position 81 and position 102 of human midkine, has been found to have excellent reactivity with and excellent neutralizing activity against human midkine. Moreover, the activity of suppressing the proliferation of tumor has been observed in the antibody having excellent neutralizing activity. The use of the antibody of the present invention makes it possible to treat cancer effectively and to detect or purify midkine efficiently.

公开(公告)号：EP2881467A1

公开(公告)日：2015-06-10

申请号：EP13825402.4

申请日：2013-07-30

申请人： National University Corporation Nagoya University ,  Medical & Biological Laboratories Co., Ltd.

发明人： KADOMATSU, Kenji ,  KISHIDA, Satoshi ,  ONO, Kenichiro ,  YAGI, Kasumi

IPC分类号： C12N15/09 ,  A61K39/395 ,  A61P35/00 ,  C07K16/18 ,  C07K16/46 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12P21/08

CPC分类号： C07K16/22 ,  C07K2317/24 ,  C07K2317/34 ,  C07K2317/55 ,  C07K2317/565 ,  C07K2317/73 ,  C07K2317/76 ,  C07K2317/90

摘要： A monoclonal antibody, which recognizes at least two amino acids among amino acids located at position 69, position 79, position 81 and position 102 of human midkine, has been found to have excellent reactivity with and excellent neutralizing activity against human midkine. Moreover, the activity of suppressing the proliferation of tumor has been observed in the antibody having excellent neutralizing activity. The use of the antibody of the present invention makes it possible to treat cancer effectively and to detect or purify midkine efficiently.

摘要翻译： 已经发现，在人类midkine的位置69，位置79，位置81和位置102的氨基酸中识别至少两个氨基酸的单克隆抗体具有优异的与人类midkine的反应性和优异的中和活性。 此外，在具有优异的中和活性的抗体中观察到抑制肿瘤增殖的活性。 使用本发明的抗体可以有效地治疗癌症并有效地检测或纯化中期因子。