利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

6 条记录 (耗时 0.014 秒)

    METHOD FOR CLONING T CELL RECEPTOR
    3.
    发明公开
    METHOD FOR CLONING T CELL RECEPTOR 审中-公开
    VERFAHREN ZUM KLONEN EINES T-ZELL-REZEPTORS

    公开(公告)号：EP2899269A1

    公开(公告)日：2015-07-29

    申请号：EP13823838.1

    申请日：2013-07-24

    申请人： National University Corporation University Of Toyama

    发明人： KISHI, Hiroyuki ,  MURAGUCHI, Atsushi ,  KOBAYASHI, Eiji ,  OZAWA, Tatsuhiko

    IPC分类号： C12N15/09 ,  C12N5/10

    CPC分类号： C12P19/34 ,  C07K14/7051 ,  C12N5/0636 ,  C12N15/85

    摘要： An object is to provide a TCR cloning system that enables not only bias-free analysis of TCR repertoires, but also collection of antigen-specific TCR α/β cDNA pairs and evaluation of functions thereof. There is provided a method for producing a gene of T cell receptor (TCR) specific to an antigen A, which comprises 1) the step of stimulating a group of T cells including a T cell specific to an antigen A or one T cell specific to an antigen A under a condition effective for amplification of a TCR gene; 2) the step of identifying a T cell specific to an antigen A among the group of T cells including a T cell specific to the antigen A, and sorting one T cell specific to the antigen A into a vessel; and 3) the step of subjecting the one activated T cell specific to the antigen A in the vessel to PCR to amplify a gene of TCR specific to the antigen A. According to the present invention, a target TCR gene can be cloned within a shorter time compared with that required by the conventional methods, for example, about ten days. Further, according to the present invention, genes of TCR α chain and β chain can be highly efficiently cloned. Under the conditions of the examples, a pair of a TCR α chain and TCR β chain could be obtained from stimulated T cells sorted as single cells at a ratio of 100%.

    摘要翻译： 目的是提供一种TCR克隆系统，其不仅能够对TCR谱系进行无偏差分析，而且能够收集抗原特异性TCR±/ / 2 cDNA对和其功能的评估。 提供了一种用于产生对抗原A特异性的T细胞受体（TCR）基因的方法，其包括：1）刺激一组T细胞的步骤，所述T细胞包括对抗原A特异性的T细胞或一种特异于T细胞的T细胞 在有效扩增TCR基因的条件下的抗原A; 2）鉴定包含对抗原A特异性的T细胞的T细胞群体中特异于抗原A的T细胞的步骤，将对抗原A特异性的1个T细胞分选成容器; 和3）将所述特异性的一种活化的T细胞对所述血管中的抗原A进行PCR的步骤，以扩增抗原A特异性的TCR基因。根据本发明，靶TCR基因可以在较短的 时间与常规方法所需的时间相比，例如约十天。 此外，根据本发明，可以高效克隆TCR±链和链的基因。 在实施例的条件下，可以从100％的比例分选为单细胞的刺激的T细胞获得一对TCR±链和TCR2链。

    METHOD FOR STIMULATING T CELL AND USE THEREOF
    6.
    发明公开
    METHOD FOR STIMULATING T CELL AND USE THEREOF 审中-公开
    刺激T细胞的方法及其用途

    公开(公告)号：EP2824182A1

    公开(公告)日：2015-01-14

    申请号：EP13758671.5

    申请日：2013-03-06

    申请人： National University Corporation University Of Toyama

    发明人： KISHI, Hiroyuki ,  MURAGUCHI, Atsushi ,  HAMANA, Hiroshi ,  KOBAYASHI, Eiji ,  OZAWA, Tatsuhiko

    IPC分类号： C12N15/09 ,  A61K39/00 ,  A61K47/42 ,  C12N5/0783 ,  C12N5/10 ,  C12Q1/02 ,  G01N37/00 ,  G01N33/53

    CPC分类号： C07K14/705 ,  A61K2039/5158 ,  C12N5/0636 ,  C12N2503/00 ,  G01N33/505 ,  G01N33/566 ,  G01N33/574 ,  G01N33/6869

    摘要： An object of the present invention is to stimulate a T cell without using a peptide/MHC tetramer. In the present invention, the step of supplying an antigen peptide to a T cell having a T cell receptor (TCR) that can recognize the antigen peptide on cell surface to form a complex of a major histocompatibility complex (MHC) molecule on the cell surface of the T cell and the antigen peptide is used, and the T cell is stimulated through recognition by TCR of the antigen peptide as the MHC molecule-antigen peptide complex on the cell surface of the same T cell. Such a stimulating and activating method would be applicable to not only T cells, but also various cells. According to the present invention, an antigen-specific T cell can be identified without establishing any antigen-specific T cell strain, and without using such a reagent as MHC/peptide tetramer. That is, a cancer-specific T cell can be efficiently and conveniently identified.

    摘要翻译： 本发明的一个目的是在不使用肽/ MHC四聚体的情况下刺激T细胞。 在本发明中，向具有可识别细胞表面的抗原肽的T细胞受体（TCR）的T细胞供给抗原肽，从而在细胞表面形成主要组织相容性复合体（MHC）分子的复合体的步骤 使用T细胞和抗原肽，通过作为同一T细胞的细胞表面的MHC分子 - 抗原肽复合物的抗原肽的TCR的识别来刺激T细胞。 这种刺激和活化方法不仅适用于T细胞，而且适用于各种细胞。 根据本发明，可以鉴定抗原特异性T细胞而不建立任何抗原特异性T细胞株，并且不使用诸如MHC /肽四聚体的试剂。 也就是说，可以有效且方便地鉴定癌症特异性T细胞。