公开(公告)号：EP2809785A1

公开(公告)日：2014-12-10

申请号：EP13703562.2

申请日：2013-02-01

申请人： Novartis AG

发明人： DELANY, Isabel ,  GUADAGNUOLO, Serafina

IPC分类号： C12N15/74 ,  C12N15/63

CPC分类号： C12N15/74 ,  A61K39/095 ,  C07K14/22 ,  C12N2800/60

摘要： New promoters are described to drive transcription in meningococcus e.g. for over-expression of protein antigens for retention in membrane vesicles. Modified porA promoters lack the wild-type poly-G sequence which can cause phase variation. Meningococcal rRNA-coding genes (e.g. for 16S rRNA) can be used to drive transcription of a protein-coding gene. These approaches can be used in combination.

摘要翻译： 描述新的启动子以驱动脑膜炎球菌中的转录。 用于蛋白质抗原的过表达以保留在膜囊泡中。 修饰的porA启动子缺乏可引起相变的野生型poly-G序列。 脑膜炎球菌rRNA编码基因（例如16S rRNA）可用于驱动蛋白质编码基因的转录。 这些方法可以组合使用。

公开(公告)号：EP2613805A2

公开(公告)日：2013-07-17

申请号：EP11764868.3

申请日：2011-09-09

申请人： Novartis AG

发明人： SERRUTO, Davide ,  PIZZA, Mariagrazia ,  DELANY, Isabel

IPC分类号： A61K39/095 ,  C12N1/00

CPC分类号： A61K39/095 ,  A61K2039/523 ,  C07K14/22 ,  C12N1/20 ,  C12N1/38 ,  C12N9/1085 ,  C12Y205/01072

摘要： A first aspect of the invention provides meningococcal outer membrane vesicles in which NHBA is over-expressed. A second aspect of the invention provides meningococcal outer membrane vesicles in which NadA is over-expressed. A third aspect of the invention provides a panel of bacterial strains, each member of which is isogenic except for a single gene which in each strain encodes a different variant of an antigen of interest.

摘要翻译： 本发明的第一方面提供了NHBA过表达的脑膜炎球菌外膜囊泡。 本发明的第二方面提供了其中NadA过度表达的脑膜炎球菌外膜囊泡。 本发明的第三方面提供了一组细菌菌株，其每个成员是同基因的，除了在每个菌株中编码感兴趣抗原的不同变体的单个基因。

公开(公告)号：EP2483390A2

公开(公告)日：2012-08-08

申请号：EP10776416.9

申请日：2010-09-30

申请人： Novartis AG

发明人： ORIENTE, Francesca ,  DELANY, Isabel

IPC分类号： C12N1/21

CPC分类号： C12N15/74 ,  A61K39/095 ,  A61K2035/11

摘要： The meningococcal
fhbp gene (encoding factor H binding protein) is naturally expressed from two independent transcripts by two differentially regulated promoters. In one transcript it is co-expressed with the neighbouring upstream gene from the P
nmb1869 promoter. The other transcript is monocistronic and is expressed from its own dedicated promoter, P
fhbp , which is activated by the global regulatory protein FNR in response to oxygen-limiting conditions. To increase expression of the monocistronic transcript a constitutively-active FNR mutant is used. The P
fhbp promoter can thus be activated, leading to over-expression of FNR-activated genes, such as
fhbp.