公开(公告)号：EP1149160B1

公开(公告)日：2006-04-19

申请号：EP00901052.1

申请日：2000-01-24

申请人： Novozymes A/S

发明人： LAUSTSEN, Mads, Aage ,  SIMPSON, Curran, Matthew ,  O'REILLY, Michael

IPC分类号： C12N9/00 ,  C12N9/50 ,  C12N9/24 ,  C07K1/00 ,  C07K1/113

CPC分类号： C12N9/2417 ,  C12N9/48

摘要： The present invention relates to a method for recovering a glycosidase or a peptidase from a culture solution from an un-solubilized state within said solution to a solubilized state within said solution.

公开(公告)号：EP0707594B1

公开(公告)日：2001-11-21

申请号：EP94920412.7

申请日：1994-06-23

申请人： Novozymes A/S

发明人： NILSSON, Birgitte, Mahler ,  LAUSTSEN, Mads, Aage ,  RANCKE-MADSEN, Anders

IPC分类号： C07K1/30

CPC分类号： C07K1/306 ,  C12N9/00 ,  Y10S435/814

摘要： The present invention relates to a method of separating a protein, in particular an enzyme, from an aqueous solution of proteins, comprising (a) providing an aqueous mixture of proteins with a salt concentration at or below 1.5 Molar, to which a water soluble polymer has been added, and (b) recovery of the protein on crystalline form.

公开(公告)号：EP0894091B1

公开(公告)日：2003-03-05

申请号：EP97914167.8

申请日：1997-03-14

申请人： Novozymes A/S

发明人： RANCKE-MADSEN, Anders ,  LAUSTSEN, Mads, Aage

IPC分类号： C07K1/30 ,  C12N9/00

CPC分类号： C07K1/306 ,  C12N9/00 ,  C12N9/2437 ,  C12Y302/01004 ,  Y10S435/816

摘要： A method for purification, and isolation in crystalline form, of a protein from a protein-containing solution comprises: treating the protein-containing solution with a crystallization-effective amount of a water-miscible organic solvent (e.g. a lower aliphatic alcohol or ketone); and isolating the protein in question in crystalline form.

公开(公告)号：EP1149160A1

公开(公告)日：2001-10-31

申请号：EP00901052.1

申请日：2000-01-24

申请人： Novozymes A/S

发明人： LAUSTSEN, Mads, Aage ,  SIMPSON, Curran, Matthew ,  O'REILLY, Michael, John

IPC分类号： C12N9/00 ,  C12N9/50 ,  C12N9/24

CPC分类号： C12N9/2417 ,  C12N9/48

摘要： The present invention relates to a method for recovering a glycosidase or a peptidase from a culture solution from an un-solubilized state within said solution to a solubilized state within said solution.

公开(公告)号：EP0892810B1

公开(公告)日：2005-11-23

申请号：EP97908137.9

申请日：1997-03-03

申请人： Novozymes A/S

发明人： NIELSSON, Stig ,  LAUSTSEN, Mads, Aage

IPC分类号： C07K1/30 ,  C12N9/00

CPC分类号： C12N9/00 ,  C07K1/306 ,  Y10S435/816

摘要： The invention deals with a method for crystallization of a protein obtained from a protein solution comprising: (a) treatment of the protein solution with a salt containing a sulphur atom having an oxidation state less than 6; (b) recovering of the protein on crystalline form.

公开(公告)号：EP1289629B1

公开(公告)日：2004-08-04

申请号：EP01933638.7

申请日：2001-05-15

申请人： Novozymes A/S

发明人： LAUSTSEN, Mads, Aage ,  NIELSEN, Sören, Bo ,  JAKOBSEN, Sune ,  HANSEN, Kim, Uhre

IPC分类号： B01D61/16 ,  C12H1/00 ,  C12P1/00

CPC分类号： A23J1/008 ,  A23V2002/00 ,  B01D3/001 ,  B01D61/147 ,  B01D61/16 ,  B01D2311/04 ,  C07K1/34 ,  C12N9/00 ,  B01D2311/103 ,  B01D2311/246 ,  B01D2311/2626 ,  A23V2300/34

摘要： A microfiltration process of a fermentation-derived product comprising adding activated carbon to a solution of the fermentation-derived product prior to or during the microfiltration process at a microfiltration process temperature of from 25 °C to 65 °C.

公开(公告)号：EP1289629A1

公开(公告)日：2003-03-12

申请号：EP01933638.7

申请日：2001-05-15

申请人： Novozymes A/S

发明人： LAUSTSEN, Mads, Aage ,  NIELSEN, S ren, Bo ,  JAKOBSEN, Sune ,  HANSEN, Kim, Uhre

IPC分类号： B01D61/16 ,  C12H1/00 ,  C12P1/00

CPC分类号： A23J1/008 ,  A23V2002/00 ,  B01D3/001 ,  B01D61/147 ,  B01D61/16 ,  B01D2311/04 ,  C07K1/34 ,  C12N9/00 ,  B01D2311/103 ,  B01D2311/246 ,  B01D2311/2626 ,  A23V2300/34

摘要： A microfiltration process of a fermentation-derived product comprising adding activated carbon to a solution of the fermentation-derived product prior to or during the microfiltration process at a microfiltration process temperature of from 25 °C to 65 °C.