公开(公告)号：EP2125865A1

公开(公告)日：2009-12-02

申请号：EP08717479.3

申请日：2008-03-06

申请人： Novozymes A/S

发明人： JAKOBSEN, Sune ,  HANSEN, Kim Uhre ,  KAASGAARD, Svend

IPC分类号： C07K1/14 ,  C12N9/52 ,  C12N9/54 ,  C12N15/31

CPC分类号： C12N9/54 ,  C07K1/145 ,  C07K2299/00

摘要： A method of solubilizing protease crystals and/or protease precipitate in a fermentation broth comprising a) diluting the fermentation broth 100-2000% (w/w); b) adding a divalent salt; and c) adjusting the pH value of the fermentation broth to a pH value below pH 5.5.

公开(公告)号：EP3606936A1

公开(公告)日：2020-02-12

申请号：EP18715649.2

申请日：2018-04-03

申请人： Novozymes A/S

发明人： GLANVILLE, Simon ,  PIND, Peter, Frode ,  JAKOBSEN, Sune ,  JOHANSEN, Lars ,  JACOBSEN, Carsten ,  ANDERSEN, Kim, Bruno ,  BEIER, Søren, Prip ,  RYPE, Jens, Ulrik

IPC分类号： C07K1/14 ,  C12N9/26 ,  C12N9/54

公开(公告)号：EP2870256A1

公开(公告)日：2015-05-13

申请号：EP13732582.5

申请日：2013-07-02

申请人： Novozymes A/S

发明人： JENSEN, Helene, Munthe ,  ELLEGAARD, Martin ,  JAKOBSEN, Sune

IPC分类号： C12P21/00 ,  C12P21/02 ,  C12N1/00 ,  A01N37/02 ,  C12N1/14

CPC分类号： C12N1/005 ,  A01N37/02 ,  C12N9/2434 ,  C12N9/2437

摘要： A method of inactivating the microbial host cell in a fermentation broth comprising an enzyme of interest and the microbial host cell producing the enzyme of interest comprising: a) Adding a fatty acid having a chain length of C4-C12 to the fermentation broth; and b) Mixing the fermentation broth for a sufficient period of time until the microbial host cell is inactivated.

公开(公告)号：EP2552934B1

公开(公告)日：2015-07-15

申请号：EP11710201.2

申请日：2011-03-25

申请人： Novozymes A/S

发明人： PIND, Peter Frode ,  GLANVILLE, Simon ,  JAKOBSEN, Sune ,  KAASGAARD, Svend

IPC分类号： C07K1/14 ,  C07K1/30

CPC分类号： C07K1/30 ,  C07K1/306

公开(公告)号：EP2125865B1

公开(公告)日：2014-07-30

申请号：EP08717479.3

申请日：2008-03-06

申请人： Novozymes A/S

发明人： JAKOBSEN, Sune ,  HANSEN, Kim Uhre ,  KAASGAARD, Svend

IPC分类号： C07K1/14 ,  C12N9/52 ,  C12N9/54 ,  C12N15/31

CPC分类号： C12N9/54 ,  C07K1/145 ,  C07K2299/00

公开(公告)号：EP1289629B1

公开(公告)日：2004-08-04

申请号：EP01933638.7

申请日：2001-05-15

申请人： Novozymes A/S

发明人： LAUSTSEN, Mads, Aage ,  NIELSEN, Sören, Bo ,  JAKOBSEN, Sune ,  HANSEN, Kim, Uhre

IPC分类号： B01D61/16 ,  C12H1/00 ,  C12P1/00

CPC分类号： A23J1/008 ,  A23V2002/00 ,  B01D3/001 ,  B01D61/147 ,  B01D61/16 ,  B01D2311/04 ,  C07K1/34 ,  C12N9/00 ,  B01D2311/103 ,  B01D2311/246 ,  B01D2311/2626 ,  A23V2300/34

摘要： A microfiltration process of a fermentation-derived product comprising adding activated carbon to a solution of the fermentation-derived product prior to or during the microfiltration process at a microfiltration process temperature of from 25 °C to 65 °C.

公开(公告)号：EP1289629A1

公开(公告)日：2003-03-12

申请号：EP01933638.7

申请日：2001-05-15

申请人： Novozymes A/S

发明人： LAUSTSEN, Mads, Aage ,  NIELSEN, S ren, Bo ,  JAKOBSEN, Sune ,  HANSEN, Kim, Uhre

IPC分类号： B01D61/16 ,  C12H1/00 ,  C12P1/00

CPC分类号： A23J1/008 ,  A23V2002/00 ,  B01D3/001 ,  B01D61/147 ,  B01D61/16 ,  B01D2311/04 ,  C07K1/34 ,  C12N9/00 ,  B01D2311/103 ,  B01D2311/246 ,  B01D2311/2626 ,  A23V2300/34

摘要： A microfiltration process of a fermentation-derived product comprising adding activated carbon to a solution of the fermentation-derived product prior to or during the microfiltration process at a microfiltration process temperature of from 25 °C to 65 °C.

公开(公告)号：EP2552934A1

公开(公告)日：2013-02-06

申请号：EP11710201.2

申请日：2011-03-25

申请人： Novozymes A/S

发明人： PIND, Peter Frode ,  GLANVILLE, Simon ,  JAKOBSEN, Sune ,  KAASGAARD, Svend

IPC分类号： C07K1/14 ,  C07K1/30

CPC分类号： C07K1/30 ,  C07K1/306

摘要： A method for producing a crystalline and/or amorphous metabolite suspension from a cell fermentation broth in a continuous centrifuge process comprising (a) adding at a separate inlet to the centrifuge the fermentation broth comprising the cells and the metabolite of interest, wherein the metabolite is partly or wholly on crystalline and/or amorphous form; (b) adding at another inlet to the centrifuge an aqueous liquid comprising a salt and/or a carbohydrate having a higher density than the cells and a lower density than the metabolite of interest in its precipitated form; (c) removing the cells at a separate outlet to the centrifuge; and (d) removing the suspension comprising the crystalline and/or amorphous metabolite of interest at another outlet to the centrifuge.