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    RECOMBINANT PRODUCTION OF ANTIMICROBIAL AGENTS
    2.
    发明公开
    RECOMBINANT PRODUCTION OF ANTIMICROBIAL AGENTS 有权
    Rekombinante Herstellung antiikrobieller Agentien

    公开(公告)号：EP1680503A1

    公开(公告)日：2006-07-19

    申请号：EP04762825.0

    申请日：2004-09-13

    申请人： Novozymes A/S

    发明人： JENSEN, Ejner, Bech ,  HÖGENHAUG, Hans-Henrik, Kristensen ,  HANSEN, Peter, Kamp ,  PEDERSEN, Poul, Erik ,  MYGIND, Per, Holse

    IPC分类号： C12N5/10 ,  A23K1/17 ,  C07K14/47 ,  C12N15/62 ,  C12N9/90 ,  A23K1/00 ,  C12N15/82 ,  A01K67/027 ,  C07K1/107

    CPC分类号： C12N15/62 ,  A01K2217/05 ,  A23K20/147 ,  A23K20/189 ,  C07K14/4723 ,  C07K2319/00 ,  C07K2319/02 ,  C12N15/79 ,  C12Q1/025

    摘要： The invention relates to the use of co-expression of an antimicrobial agent and an enzyme, with a view to improving yield and/or overall production economy. Examples of antimicrobial agents are antimicrobial peptides such as lactoferricins and antimicrobial enzymes such as lysozyme and glucose oxidase, and examples of enzymes are endoglucanase, xylanase, phytase, protease, galactanase, mannanase, dextranase, alpha-galactosidase, pectate lyase, alpha-amylase and glucoamylase. A fusion product comprising the antimicrobial agent and the enzyme and a cleavable linker is novel, and can be used in animal feed and animal feed additives. The invention also describes the use of a protection domain wherein at least 50% of the amino acid residues comprised in the peptide protection domain are D (Asp) and/or E (Glu). The protection or quenching domain serves to temporarily and reversibly inactivate the antimicrobial peptide during the expression.

    摘要翻译： 本发明涉及抗微生物剂和酶的共表达的用途，以提高产量和/或整体生产经济性。 抗微生物剂的实例是抗微生物肽如乳铁蛋白和抗菌酶如溶菌酶和葡萄糖氧化酶，酶的实例是内切葡聚糖酶，木聚糖酶，植酸酶，蛋白酶，半乳聚糖酶，甘露聚糖酶，葡聚糖酶，α-半乳糖苷酶，果胶酸裂合酶，α-淀粉酶和 糖化酶。 包含抗微生物剂和酶和可切割接头的融合产物是新颖的，并且可以用于动物饲料和动物饲料添加剂中。 本发明还描述了保护结构域的用途，其中肽保护结构域中包含的至少50％的氨基酸残基是D（Asp）和/或E（Glu）。 保护或猝灭结构域用于在表达过程中暂时和可逆地灭活抗微生物肽。