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    PROCESSES FOR PRODUCING A FERMENTATION PRODUCT
    1.
    发明公开
    PROCESSES FOR PRODUCING A FERMENTATION PRODUCT 审中-公开
    生产发酵产品的过程

    公开(公告)号：EP1745122A2

    公开(公告)日：2007-01-24

    申请号：EP05711438.1

    申请日：2005-01-14

    申请人： Novozymes North America, Inc. ,  Novozymes A/S

    发明人： ALLAIN, Eric ,  WENGER, Kevin, S. ,  BISGARD-FRANTZEN, Henrik

    IPC分类号： C12N1/00

    CPC分类号： C12P7/06 ,  Y02E50/17

    摘要： The present invention relates to processes for producing a fermentation product, such as ethanol, from milled starch-containing material comprising (a) saccharifying the milled starch-containing material with a glucoamylase having an amino acid sequence shown in SEQ ID NO: 2, or a glucoamylase being at least 70% identical thereto, at a temperature below the initial gelatinization temperature of said starch-containing material, (b) fermenting using a fermenting organism.

    摘要翻译： 本发明涉及由研磨的含淀粉材料生产发酵产物如乙醇的方法，包括（a）用具有SEQ ID NO：2所示氨基酸序列的葡糖淀粉酶糖化磨碎的含淀粉材料，或 在低于所述含淀粉材料的初始糊化温度的温度下，葡糖淀粉酶与其至少70％相同，（b）使用发酵生物进行发酵。

    A PROCESS OF PRODUCING A FERMENTATION PRODUCT
    2.
    发明公开
    A PROCESS OF PRODUCING A FERMENTATION PRODUCT 审中-公开
    法生产发酵产物

    公开(公告)号：EP1751295A2

    公开(公告)日：2007-02-14

    申请号：EP05754334.0

    申请日：2005-05-11

    申请人： Novozymes North America, Inc. ,  Novozymes A/S

    发明人： BHARGAVA, Swapnil ,  FRISNER, Henrik ,  BISGARD-FRANTZEN, Henrik ,  TAMS, Jeppe Wegener

    IPC分类号： C12P7/06

    CPC分类号： C12P7/10 ,  C12P19/14 ,  Y02E50/16 ,  Y02E50/17

    摘要： The invention relates to a process of producing a fermentation product, such as ethanol, from starch-containing material, including i) subjecting starch-containing material to an alpha-amylase, ii) subjecting the material obtained in step i) to an alpha-glucosidase and/or a maltose-generating enzyme, and iii) fermenting the material in the presence of a fermenting organism, such as yeast. Alternatively the invention relates to a process of producing a fermentation product from starch-containing material, preferably granular starch, which process comprises: a) subjecting starch-containing material to an alpha-glucosidase and optionally a glucose-generating and/or maltose-generating enzyme, and b) fermenting the material in the presence of a fermenting organism.

    AMYLASE VARIANTS
    3.
    发明授权
    AMYLASE VARIANTS 无效
    AMYLASE变种

    公开(公告)号：EP0815208B1

    公开(公告)日：2006-08-02

    申请号：EP96900894.5

    申请日：1996-02-05

    申请人： Novozymes A/S

    发明人： BISGARD-FRANTZEN, Henrik ,  SVENDSEN, Allan ,  BORCHERT, Torben Vedel

    IPC分类号： C12N9/28 ,  C11D3/386

    CPC分类号： C12C5/004 ,  C11D3/386 ,  C11D3/38672 ,  C11D3/38681 ,  C12N9/2417 ,  D06L1/14 ,  D21C5/005 ,  D21C5/022 ,  D21C5/027 ,  D21H17/005 ,  D21H17/28 ,  Y02E50/17 ,  Y02W30/648

    摘要： A variant of a parent α-amylase, which parent α-amylase (i) has an amino acid sequence selected from the amino acid sequences shown in SEQ ID No. 1, SEQ ID No. 2, SEQ ID No. 3, and SEQ ID No. 7, respectively; or (ii) displays at least 80 % homology with one or more of these amino acid sequences; and/or displays immunological cross-reactivity with an antibody raised against a α-amylase having one of these amino acid sequences; and/or is encoded by a DNA sequence which hybridizes with the same probe as a DNA sequence encoding a α-amylase having one of these amino acid sequences; in which variant: (a) at least one amino acid residue of the parent α-amylase has been deleted; and/or (b) at least one amino acid residue of the parent α-amylase has been replaced by a different amino acid residue; and/or (c) at least one amino acid residue has been inserted relative to the parent α-amylase; the variant having α-amylase activity and exhibiting at least one of the following properties relative to the parent α-amylase: increased thermostability; increased stability towards oxidation; and reduced Ca2+ dependency; with the proviso that the amino acid sequence of the variant is not identical to any of the amino acid sequences shown in SEQ ID No. 1, SEQ ID No. 2, SEQ ID No. 3 and SEQ ID No. 7, respectively.

    摘要翻译： 亲本α-淀粉酶（i）具有选自SEQ ID No.1，SEQ ID No.2，SEQ ID No.3和SEQ ID No.3所示氨基酸序列的氨基酸序列的亲本α-淀粉酶变体 ID分别为7; 或（ii）与一个或多个这些氨基酸序列显示至少80％的同源性; 和/或显示与针对具有这些氨基酸序列之一的α-淀粉酶的抗体的免疫交叉反应性; 和/或由与编码具有这些氨基酸序列之一的α-淀粉酶的DNA序列的相同探针杂交的DNA序列编码; 其中该变体：（a）亲本α-淀粉酶的至少一个氨基酸残基已被缺失; 和/或（b）亲本α-淀粉酶的至少一个氨基酸残基已被不同的氨基酸残基置换; 和/或（c）相对于亲本α-淀粉酶插入了至少一个氨基酸残基; 所述变体具有α-淀粉酶活性并显示相对于亲本α-淀粉酶至少一种以下性质：增加的热稳定性; 增加对氧化的稳定性; 并减少Ca2 +依赖性; 条件是变体的氨基酸序列与分别在SEQ ID No.1，SEQ ID No.2，SEQ ID No.3和SEQ ID No.7中所示的任何氨基酸序列不同。

    ALPHA-AMYLASE VARIANTS
    4.
    发明授权
    ALPHA-AMYLASE VARIANTS 有权
    ALPHA-淀粉酶变体

    公开(公告)号：EP1165762B1

    公开(公告)日：2007-04-25

    申请号：EP00912415.7

    申请日：2000-03-28

    申请人： Novozymes A/S

    发明人： ANDERSEN, Carsten ,  JOERGENSEN, Christel, Thea ,  BISGARD-FRANTZEN, Henrik ,  SVENDSEN, Allan ,  KJAERULFF, Soeren

    IPC分类号： C12N9/28 ,  C11D3/386

    CPC分类号： C12N9/2417 ,  C07K2319/00 ,  Y02E50/17

    摘要： The invention relates to a variant of a parent Termamyl-like alpha-amylase, which variant exhibits altered properties, in particular reduced capability of cleaving a substrate close to the branching point, and improved substrate specificity and/or improved specific activity relative to the parent alpha-amylase. The variant of the parent Termamyl-like alpha-amylase, comprised an alternation at one or more positions selected from the group of W13, G48, T49, S50, Q51, A52, D53, V54, G57, G107, G108, A111, S168 and M197.

    摘要翻译： 本发明涉及亲代Termamyl样α-淀粉酶的变体，所述变体表现出改变的性质，特别是降低了接近分支点的底物的切割能力，并且相对于亲本改进了底物特异性和/或改善的比活性 α-淀粉酶。 亲代Termamyl样α-淀粉酶的变体在选自W13，G48，T49，S50，Q51，A52，D53，V54，G57，G107，G108，A111，S168的一个或多个位置处包含交替 和M197。

    A METHOD OF DESIGNING ALPHA-AMYLASE MUTANTS WITH PREDETERMINED PROPERTIES
    8.
    发明授权
    A METHOD OF DESIGNING ALPHA-AMYLASE MUTANTS WITH PREDETERMINED PROPERTIES 失效
    用于α-淀粉酶突变体的具有预定特性的设计方法

    公开(公告)号：EP0808363B1

    公开(公告)日：2009-05-27

    申请号：EP96900895.2

    申请日：1996-02-05

    申请人： Novozymes A/S

    发明人： SVENDSEN, Allan ,  BISGARD-FRANTZEN, Henrik ,  BORCHERT, Torben, Vedel

    IPC分类号： C12N9/28 ,  C12N15/56

    CPC分类号： C12N9/2417 ,  C11D3/386

    摘要： A method of constructing a variant of a parent Termamyl-like α-amylase, which variant has α-amylase activity and at least one altered property as compared to the parent α-amylase, comprises i) analysing the structure of the parent Termamyl-like α-amylase to identify at least one amino acid residue or at least one structural part of the Termamyl-like α-amylase structure, which amino acid residue or structural part is believed to be of relevance for altering the property of the parent Termamyl-like α-amylase (as evaluated on the basis of structural or functional considerations), ii) constructing a Termamyl-like α-amylase variant, which as compared to the parent Termamyl-like α-amylase, has been modified in the amino acid residue or structural part identified in i) so as to alter the property, and iii) testing the resulting Termamyl-like α-amylase variant for the property in question.

    AMYLASE VARIANTS
    9.
    发明授权
    AMYLASE VARIANTS 失效
    淀粉酶

    公开(公告)号：EP0722490B1

    公开(公告)日：2006-02-22

    申请号：EP94928775.9

    申请日：1994-10-05

    申请人： Novozymes A/S

    发明人： BISGARD-FRANTZEN, Henrik ,  BORCHERT, Torben, Vedel ,  SVENDSEN, Allan ,  THELLERSEN, Marianne ,  VAN DER ZEE, Pia

    IPC分类号： C12N9/28 ,  C12N15/56 ,  C11D3/386

    CPC分类号： C12N9/2417 ,  C11D3/386 ,  D06L1/14

    摘要： A variant of a parent α-amylase enzyme having an improved washing and/or dishwashing performance as compared to the parent enzyme, wherein one or more amino acid residues of the parent enzyme have been replaced by a different amino acid residue and/or wherein one or more amino acid residues of the parent α-amylase have been deleted and/or wherein one or more amino acid residues have been added to the parent α-amylase enzyme, provided that the variant is different from one in which the methionine residue in position 197 of a parent B. licheniformis α-amylase has been replaced by alanine or threonine, as the only modification being made. The variant may be used for washing and dishwashing.