公开(公告)号：EP1416051B1

公开(公告)日：2011-06-08

申请号：EP02738905.5

申请日：2002-07-02

申请人： ORIENTAL YEAST CO., LTD.

发明人： KAWAI, Shigeyuki ,  MURATA, Kousaku ,  MATSUKAWA, Hirokazu, c/o Oriental Yeast Co., Ltd. ,  TOMISAKO, Shoichi, c/o Oriental Yeast Co., Ltd. ,  ANDO, Yoshio, c/o Oriental Yeast Co., Ltd. ,  MATSUO, Yuhsi, c/o Oriental Yeast Co., Ltd.

IPC分类号： C12P19/36 ,  C12N9/12 ,  C12N15/54

CPC分类号： C12P19/36

摘要： A novel process for producing nicotinamide adenine dinucleotide phosphate (NADP) which comprises using polyphosphoric acid or its salt and nicotinamide adenine dinucleotide (NAD+) as a substrate and phosphorylating the substrate with the use of a polyphosphoric acid-depending NAD+ kinase originating in a mycobacterium, characterized in that the liquid reaction mixture contains fro 0.1 to 15% by weight of polyphosphoric acid or its salt and from 5 to 150 mM of a divalent metal ion.

公开(公告)号：EP1416051A1

公开(公告)日：2004-05-06

申请号：EP02738905.5

申请日：2002-07-02

申请人： ORIENTAL YEAST CO., LTD.

发明人： KAWAI, Shigeyuki ,  MURATA, Kousaku ,  MATSUKAWA, Hirokazu, c/o Oriental Yeast Co., Ltd. ,  TOMISAKO, Shoichi, c/o Oriental Yeast Co., Ltd. ,  ANDO, Yoshio, c/o Oriental Yeast Co., Ltd. ,  MATSUO, Yuhsi, c/o Oriental Yeast Co., Ltd.

IPC分类号： C12N15/54 ,  C12P19/36

CPC分类号： C12P19/36

摘要： The present invention provides a novel process for preparing nicotinamide adenine dinucleotide phosphate (NADP). The process of the present invention comprises performing phosphorylation using a polyphosphoric acid or a salt thereof and nicotinamide adenine dinucleotide (NAD + ) as substrates in the presence of a polyphosphate-dependent NAD + kinase from a Mycobacterium, wherein the reaction solution contains 0.1-15% by weight of the polyphosphoric acid or a salt thereof, and 5-150 mM of a divalent metal ion.

摘要翻译： 产生烟酰胺腺嘌呤二核苷酸磷酸（NADP）的方法包括使用多磷酸或其盐和烟酰胺腺嘌呤二核苷酸（NAD +>）作为底物，以分枝杆菌为起始的多磷酸依赖性NAD +>激酶用于磷酸化，其中 含有0.1-15重量％多磷酸或其盐的液体反应混合物和5至15mM二价金属离子。