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公开(公告)号：EP2752495B1
公开(公告)日：2020-03-25
申请号：EP12818440.5
申请日：2012-07-16
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2.发明公开METHOD FOR DETERMINING THE ACTIVITY OF A PROTEOLYTIC ENZYME (VARIANTS), DEVICE FOR THE IMPLEMENTATION OF SAME AND DIAGNOSTIC METHOD 审中-公开一种用于确定蛋白水解酶的活性（变化）DEVICE用于实现该方法和诊断步骤
公开(公告)号：EP2752495A2
公开(公告)日：2014-07-09
申请号：EP12818440.5
申请日：2012-07-16
发明人： ATAULLAKHANOV, Fazoil Inoyatovichon , DASHKEVICH, Nataljya Mikhajlovna , OVANESOV, Mikhail Vladimirovich , SARBASH, Vasilii Ivanovich , PANTELEEV, Mikhail Aleksandrovich , KARAMZIN, Sergey Sergeevich , KONDRATOVICH, Andrey Yurjevich
CPC分类号： C12Q1/56 , C12Q1/37 , G01N21/6408 , G01N21/6456 , G01N2021/6439 , G01N2333/95 , G01N2333/974
摘要： The invention provides methods for determining the spatiotemporal distribution of activity of a proteolytic enzyme in a heterogeneous system, said method comprising the steps of: (i)providing an in-vitro system, (ii) combining a fluorogenic / chromogeneous / luminescent substrate, (iii) illuminating the sample; (iv) recording the spatial distribution of the signals (v) calculating the spatiotemporal distribution of the proteolytic enzyme activity whereby each step is detailed in the specification and whereby the step of calculating includes correcting the spatiotemporal distribution by using at least one numerical algorithm for reducing noise and distortion of said signal.
The methods are useful in medicine and biology and can be used, in particular, for diagnostic and research purposes in order to determine the parameters of blood coagulation system and the components thereof.
The invention further provides for devices useful in performing the above methods.摘要翻译： 本发明提供了用于确定性的采矿方法的蛋白水解酶的活性在非均相系统中的时空分布，所述方法包括如下步骤：（i）提供在体外系统中，（ii）将一个荧光/ chromogeneous /发光底物，（ ⅲ）照射所述样品; （四）记录的信号的空间分布（V）计算该蛋白水解酶的活性，由此每个步骤在说明书中详述并的时空分布，由此计算步骤包括：通过使用至少一个数字算法用于减小校正时空分布 噪声和Said信号的失真。 的方法是在医学和生物学有用并且可以用于，特别是用于诊断和研究目的以确定性矿井血液凝固系统的参数和它们的组分。 本发明进一步提供用于在执行上述方法中有用的设备。
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