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公开(公告)号：EP2307540B1
公开(公告)日：2017-04-19
申请号：EP09784644.8
申请日：2009-07-06
发明人： JAYASINGHE, Lakmal , BAYLEY, Hagan , CHELEY, Stephen , MCKEOWN, Brian , WHITE, James , CLARKE, James
CPC分类号： C12Q1/6869 , C07K14/31 , C12N9/1247 , C12N9/1252 , C12N9/127 , C12N9/1276 , C12N9/16 , C12N9/22 , C12N9/52 , C12N9/90 , C12N9/96 , C12Q2565/631
摘要： The invention relates to constructs comprising a transmembrane protein pore subunit and a nucleic acid handling enzyme. The pore subunit is covalently attached to the enzyme such that both the subunit and enzyme retain their activity. The constructs can be used to generate transmembrane protein pores having a nucleic acid handling enzyme attached thereto. Such pores are particularly useful for sequencing nucleic acids. The enzyme handles the nucleic acid in such a way that the pore can detect its component nucleotides by stochastic sensing.
摘要翻译： 本发明涉及包含跨膜蛋白质孔亚基和核酸处理酶的构建体。 孔亚基共价连接到酶，使得亚基和酶保留其活性。 构建体可用于产生具有附着于其上的核酸处理酶的跨膜蛋白孔。 这样的孔特别可用于测序核酸。 酶以这样的方式处理核酸，使得孔可以通过随机感测检测其成分核苷酸。
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公开(公告)号：EP2310534B1
公开(公告)日：2018-09-05
申请号：EP09784654.7
申请日：2009-07-06
IPC分类号： C12Q1/68 , C07K14/245 , C12Q1/6869
CPC分类号： C07K14/245 , C12Q1/6869 , C12Q2565/631
摘要： The invention relates to a mutant α-hemolysin (α-HL) pore which is useful for detecting one or more nucleotides by stochastic sensing. The pore is particularly useful for sequencing DNA or RNA. A molecular adaptor that allows detection of the nucleotide(s) is covalently attached to the pore. The pore is specifically modified to facilitate positioning of the adaptor and may be modified to facilitate covalent attachment.
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公开(公告)号：EP2310534A1
公开(公告)日：2011-04-20
申请号：EP09784654.7
申请日：2009-07-06
IPC分类号： C12Q1/68
CPC分类号： C07K14/245 , C12Q1/6869 , C12Q2565/631
摘要： The invention relates to a mutant α-hemolysin (α-HL) pore which is useful for detecting one or more nucleotides by stochastic sensing. The pore is particularly useful for sequencing DNA or RNA. A molecular adaptor that allows detection of the nucleotide(s) is covalently attached to the pore. The pore is specifically modified to facilitate positioning of the adaptor and may be modified to facilitate covalent attachment.
摘要翻译： 本发明涉及用于通过随机感测来检测一个或多个核苷酸的突变α-溶血素（α-HL）孔。 孔对DNA或RNA测序特别有用。 允许检测核苷酸的分子接头共价连接到孔。 孔被特异性修饰以便于衔接子的定位并且可以被修饰以促进共价连接。
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公开(公告)号：EP2307540A1
公开(公告)日：2011-04-13
申请号：EP09784644.8
申请日：2009-07-06
发明人： JAYASINGHE, Lakmal , BAYLEY, Hagan , CHELEY, Stephen , MCKEOWN, Brian , WHITE, James , CLARKE, James
CPC分类号： C12Q1/6869 , C07K14/31 , C12N9/1247 , C12N9/1252 , C12N9/127 , C12N9/1276 , C12N9/16 , C12N9/22 , C12N9/52 , C12N9/90 , C12N9/96 , C12Q2565/631
摘要： The invention relates to constructs comprising a transmembrane protein pore subunit and a nucleic acid handling enzyme. The pore subunit is covalently attached to the enzyme such that both the subunit and enzyme retain their activity. The constructs can be used to generate transmembrane protein pores having a nucleic acid handling enzyme attached thereto. Such pores are particularly useful for sequencing nucleic acids. The enzyme handles the nucleic acid in such a way that the pore can detect its component nucleotides by stochastic sensing.
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