公开(公告)号：EP0533909A4

公开(公告)日：1993-06-30

申请号：EP92910887

申请日：1992-03-20

申请人： PERSEPTIVE BIOSYSTEMS, INC.

发明人： AFEYAN, NOUBAR, B. ,  REGNIER, FRED, E.

IPC分类号： B01D15/08 ,  G01N27/447 ,  G01N30/14 ,  G01N30/46 ,  G01N30/52 ,  G01N30/62 ,  G01N30/80 ,  G01N30/82 ,  G01N30/86 ,  G01N30/88

CPC分类号： G01N30/461 ,  B01D15/08 ,  G01N27/44717 ,  G01N30/62 ,  G01N30/82 ,  G01N2030/143 ,  G01N2030/524 ,  G01N2030/625 ,  G01N2030/8813 ,  G01N2030/8831

摘要： Disclosed is a method and apparatus for the rapid identification of a solute of interest in an effluent stream (14). The method involves separation of the solutes in the effluent (14) and identification of a particular solute of interest by its selective subtraction from the effluent stream (14). The effluent stream (14) from a first system (12) capable of partitioning the components of the mixture is passed through a detector (16) to produce a first output (18) which describes the temporal and/or spacial sequence of the mixture components exiting the first system (12). The effluent stream (14) is then passed through a second system (20) capable of selectively extracting a solute of interest from said effluent stream (14) and is then passed through a detector (16).

公开(公告)号：EP0442977A4

公开(公告)日：1991-12-18

申请号：EP90903657

申请日：1990-02-20

申请人： PERSEPTIVE BIOSYSTEMS, INC.

发明人： AFEYAN, NOUBAR, B. ,  REGNIER, FRED, E. ,  DEAN, ROBERT, C., JR.

IPC分类号： B01J20/281 ,  B01D15/08 ,  B01D15/34 ,  B01J20/282 ,  B01J20/285 ,  G01N30/52 ,  G01N30/88

CPC分类号： B01J20/282 ,  B01D15/345 ,  B01J20/28095 ,  B01J2220/54 ,  G01N2030/528

摘要： Disclosed are chromatography methods and matrix geometries which permit high resolution, high productivity separation of mixtures of solutes, particularly biological materials. The method involves passing fluids through specially designed chromatography matrices at high flow rates. The matrices define first and second interconnected sets of pores and a high surface area for solute interaction in fluid communication with the members of the second set of pores (14). The first and second sets of pores are embodied, for example, as the interstices among particles and throughpores (14) within the particles. The pores are dimensioned such that, at achievable high fluid flow rates, convective flow occurs in both pore sets, and the convective flow rate exceeds the rate of solute diffusion in the second pore set. This approach couples convective and diffusive mass transport to and from the active surface and permits increases in fluid velocity without the normally expected bandspreading.