利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

198 条记录 (耗时 0.027 秒)

    BUMP ARRAY DEVICE HAVING ASYMMETRIC GAPS FOR SEGREGATION OF PARTICLES
    1.
    发明公开
    BUMP ARRAY DEVICE HAVING ASYMMETRIC GAPS FOR SEGREGATION OF PARTICLES 审中-公开
    具有用于粒子分离的不对称孔的BUMP阵列装置

    公开(公告)号：EP3196642A1

    公开(公告)日：2017-07-26

    申请号：EP17160810.2

    申请日：2009-07-24

    申请人： The Trustees of Princeton University

    发明人： STURM, James C. ,  AUSTIN, Robert ,  LOUTHERBACK, Kevin ,  MORTON, Keith ,  PUCHALLA, Jason

    IPC分类号： G01N30/00 ,  G01N30/60 ,  G01N27/447

    CPC分类号： B03B5/00 ,  B01L3/502753 ,  G01N27/44791 ,  G01N30/0005 ,  G01N30/6069 ,  G01N30/6095 ,  G01N2030/528

    摘要： The disclosure relates to obstacle array devices (also known as bump array devices) for separating populations of particles by size. Improvements over previous obstacle array devices are realized by causing the fluid velocity profile across gaps between obstacles to be asymmetrical with respect to the plane that bisects the gap and is parallel to the direction of bulk fluid flow. Such asymmetry can be achieved by selecting the shape(s) of the obstacles bounding the gap such that the portions of the obstacles upstream from, downstream from, or bridging the narrowest portion of the gap are asymmetrical with respect to that plane. Improvements are also realized by using obstacles that have sharp edges bounding the gaps. Other improvements are realized by selecting obstacle shapes such that the critical particle dimensions defined by the gaps in two different fluid flow directions differ.

    摘要翻译： 本公开涉及用于按尺寸分离颗粒群体的障碍物阵列装置（也称为凸块阵列装置）。 通过使跨越障碍物之间的间隙的流体速度分布相对于平分间隙且平行于整体流体流动的方向的平面不对称来实现对先前障碍物阵列装置的改进。 这种不对称可以通过选择限定间隙的障碍物的形状来实现，使得障碍物的上游，下游或桥接间隙的最窄部分的部分相对于该平面不对称。 通过使用边界尖锐的障碍物也可以实现改进。 其他改进通过选择障碍物形状来实现，使得由两个不同流体流动方向上的间隙限定的临界颗粒尺寸不同。

    PURIFICATION COLUMNS AND METHODS
    3.
    发明公开
    PURIFICATION COLUMNS AND METHODS 审中-公开
    REINIGUNGSSÄULENUND VERFAHREN

    公开(公告)号：EP3117208A1

    公开(公告)日：2017-01-18

    申请号：EP15762208.5

    申请日：2015-03-13

    申请人： Halaka, Folim G.

    发明人： Halaka, Folim G.

    IPC分类号： G01N30/02 ,  G01N30/60 ,  B01J20/22 ,  B01J20/24 ,  B01J20/26 ,  B01J20/28 ,  B01J20/30

    CPC分类号： B01J20/286 ,  B01J20/3204 ,  B01J20/321 ,  B01J20/3274 ,  B01J20/3282 ,  B01J2220/82 ,  B01J2220/825 ,  G01N30/52 ,  G01N30/6043 ,  G01N2030/524 ,  G01N2030/525 ,  G01N2030/527 ,  G01N2030/528

    摘要： Devices and associated processes suitable for small and large scale purification of molecules in a fluid, and provides embodiments that overcome the difficulties of transitioning purification from research stages to production scale-up. The invention relates to the formation and characteristics of substantially uniform, continuous, and homogeneous porous separation matrices. The matrices are in the form of a polymeric layer with substantially uniform thickness and porosity. The polymeric layer is formed for the purpose of providing convective flow through the separation matrix.

    摘要翻译： 适用于流体中分子的小型和大规​​模纯化的装置和相关方法，并且提供了克服从研究阶段向生产放大转移纯化困难的实施例。 本发明涉及基本上均匀的，连续的和均匀的多孔分离基质的形成和特征。 基质是具有基本均匀的厚度和孔隙率的聚合物层的形式。 形成聚合物层用于提供通过分离基质的对流流动。

    METHOD FOR SEPARATING POLYNUCLEOTIDES USING MONOLITHIC CAPILLARY COLUMNS
    7.
    发明授权
    METHOD FOR SEPARATING POLYNUCLEOTIDES USING MONOLITHIC CAPILLARY COLUMNS 有权
    PROCESS FOR POLYNUKLEOTIDIONEN通过毛细管整体分离

    公开(公告)号：EP1235852B1

    公开(公告)日：2011-06-15

    申请号：EP01906697.6

    申请日：2001-01-25

    申请人： TRANSGENOMIC, Inc.

    发明人： HUBER, Christian ,  OBERACHER, Herbert ,  PREMSTALLER, Andreas

    IPC分类号： C07K1/36 ,  B01D15/08 ,  B01J20/26

    CPC分类号： B01J20/285 ,  B01D15/366 ,  B01J20/26 ,  B01J20/261 ,  B01J20/28014 ,  B01J20/28042 ,  B01J20/281 ,  B01J20/287 ,  B01J20/3064 ,  B01J20/327 ,  B01J2220/54 ,  B01J2220/82 ,  B01J2220/84 ,  G01N30/02 ,  G01N30/48 ,  G01N2030/525 ,  G01N2030/528

    摘要： Methods and devices based on capillary monolithic columns, preferably consisting of an underivatized poly(styrene-divinylbenzene) monolith, for separating a mixture of polynucleotides by ion pair-reverse phase-high performance chromatography (IP-RP-HPLC). In various aspects of the method and device the monolith is characterized by one or more of the following: the monolith is contained within a capillary tube; the monolith is immobilized by covalent attachment at the inner wall of the tube; the tube is devoid of retaining frits; the monolith is characterized by having above 10,000 theoretical plates per meter and preferably above 200,000 theoretical plates per meter; the method uses a mobile phase which is devoid of EDTA; the monolith has a surface morphology that is rugulose or brush-like; the chromatographic surfaces of the monolith are non-porous; the monolith has channels sufficiently large for convective flow of the mobile phase; the monolith is formed from a polymerization mixture including underivatized styrene, a crosslinking agent, and a porogen, wherein the porogen includes tetrahydrofuran. The monolith can be incorporated into a miniaturized chromatography system which can be coupled to a mass spectrometer for on-line separation and mass determination of single- or double-stranded polynucleotides.