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1.发明公开AMINO POLYOL AMINE OXIDASE POLYNUCLEOTIDES AND RELATED POLYPEPTIDES AND METHODS OF USE 审中-公开氨基多元醇胺氧化AND的多核苷酸相关的多肽及其用途
公开(公告)号：EP1097218A1
公开(公告)日：2001-05-09
申请号：EP99933788.4
申请日：1999-07-08
CPC分类号： C12N9/0022 , A23K10/14 , A23K10/18 , A23K30/18 , C07K2319/00 , C12N9/16 , C12N15/8209 , C12N15/8242 , C12N15/8282 , G01N2333/906
摘要： The present invention provides polynucleotides and related polypeptides of the enzyme APAO isolated from Exophiala spinifera. Additionally, the polynucleotide encoding for the APAO enzyme can be used to transform plant cells normally susceptible to Fusarium or other toxin-producing fungus infection. Plants can be regenerated from the transformed plant cells. Additionally, the present invention provides for expressing both APAO and a fumonisin esterase in a transgenic plant. In this way, a transgenic plant can be produced with the capability of degrading fumonisin, as well as with the capability of producing the degrading enzymes. In addition, the present invention provides methods for producing the APAO enzyme in both prokaryotic and non-plant eukaryotic systems. Methods for detoxification in grain, grain processing, silage, food crops and in animal feed and rumen microbes are also disclosed.
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公开(公告)号：EP0988383A1
公开(公告)日：2000-03-29
申请号：EP98933188.9
申请日：1998-07-07
发明人： DUVICK, Jonathan , MADDOX, Joyce, R. , ROOD, Tracy, A. , WANG, Xun , BOWEN, Benjamin, A. , GILLIAM, Jacob, T.
CPC分类号： A01N63/00 , C12N9/00 , C12N9/16 , C12N9/18 , C12N15/8209 , C12N15/8242 , C12N15/8282
摘要： Methods for identifying organisms capable of degrading fumonisin. Fumonisin can be incorporated into culture medium for selection of organisms resistant to fumonisin and/or capable of growing on fumonisin as a sole carbon source. Using this method, several organisms have been identified. These organisms can be used to isolate the enzymes and the genes responsible for conferring fumonisin-resistance. The gene can be cloned and inserted into a suitable expression vector so that the protein can be further characterized. Additionally, the DNA encoding for fumonisin degrading enzymes can be used to transform plant cells normally susceptible to Fusarium or other toxin-producing fungus infections. Plants can be regenerated from the transformed plant cells. In this way, a transgenic plant can be produced with the capability of degrading fumonisin, as well as with the capability of producing the degrading enzymes. Methods for detoxification in grain, grain processing, silage, food crops and in animal feed and rumen microbes are also disclosed.
摘要翻译： 用于鉴定能够降解伏马菌素的生物体的方法。 可以将伏马菌素掺入到培养基中以选择对伏马菌素有抗性和/或能够作为唯一碳源在伏马菌素上生长的生物体。 使用这种方法，已经确定了几种生物。 这些生物体可用于分离酶和负责赋予伏马菌素抗性的基因。 该基因可被克隆并插入合适的表达载体中，以便可进一步表征蛋白质。 此外，编码伏马菌素降解酶的DNA可用于转化通常对镰刀菌或其他产生毒素的真菌感染敏感的植物细胞。 植物可以从转化的植物细胞中再生。 通过这种方式，可以生产具有降解伏马菌素能力的转基因植物以及生产降解酶的能力。 还公开了谷物，谷物加工，青贮饲料，粮食作物以及动物饲料和瘤胃微生物的解毒方法。
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公开(公告)号：EP2027276B1
公开(公告)日：2011-02-02
申请号：EP07845231.5
申请日：2007-05-15
申请人： Pioneer Hi-Bred International, Inc. , E.I. DU PONT DE NEMOURS AND COMPANY , The Regents of the University of California
发明人： ALTIER, Daniel, J. , CRANE, Virginia, C. , GILLIAM, Jacob, T. , HUNTER-CEVERA, Jennie , PRESNAIL, James, K. , SCHEPERS, Eric, J. , SIMMONS, Carl, R. , TOROK, Tamas , YALPANI, Nasser , ELLANSKAYA, I.A.
IPC分类号： C12N15/82 , C07K14/385
CPC分类号： C12N15/8282
摘要： Compositions and methods for protecting a plant from a pathogen, particularly a fungal pathogen, are provided. Compositions include amino acid sequences, and variants and fragments thereof, for antipathogenic polypeptides that were isolated from fungal fermentation broths. Nucleic acids that encode the antipathogenic polypeptides are also provided. A method for inducing pathogen resistance in a plant using the nucleotide sequences disclosed herein is further provided. The method comprises introducing into a plant an expression cassette comprising a promoter operably linked to a nucleotide sequence that encodes an antipathogenic polypeptide of the invention. Compositions comprising an antipathogenic polypeptide or a transformed microorganism comprising a nucleic acid of the invention in combination with a carrier and methods of using these compositions to protect a plant from a pathogen are further provided. Transformed plants, plant cells, seeds, and microorganisms comprising a nucleotide sequence that encodes an antipathogenic polypeptide of the invention are also disclosed.
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公开(公告)号：EP2027276A2
公开(公告)日：2009-02-25
申请号：EP07845231.5
申请日：2007-05-15
申请人： Pioneer Hi-Bred International Inc. , E.I. DU PONT DE NEMOURS AND COMPANY , The Regents of the University of California
发明人： ALTIER, Daniel, J. , CRANE, Virginia, C. , GILLIAM, Jacob, T. , HUNTER-CEVERA, Jennie , PRESNAIL, James, K. , SCHEPERS, Eric, J. , SIMMONS, Carl, R. , TOROK, Tamas , YALPANI, Nasser , ELLANSKAYA, I.A.
IPC分类号： C12N15/82 , C07K14/385
CPC分类号： C12N15/8282
摘要： Compositions and methods for protecting a plant from a pathogen, particularly a fungal pathogen, are provided. Compositions include amino acid sequences, and variants and fragments thereof, for antipathogenic polypeptides that were isolated from fungal fermentation broths. Nucleic acids that encode the antipathogenic polypeptides are also provided. A method for inducing pathogen resistance in a plant using the nucleotide sequences disclosed herein is further provided. The method comprises introducing into a plant an expression cassette comprising a promoter operably linked to a nucleotide sequence that encodes an antipathogenic polypeptide of the invention. Compositions comprising an antipathogenic polypeptide or a transformed microorganism comprising a nucleic acid of the invention in combination with a carrier and methods of using these compositions to protect a plant from a pathogen are further provided. Transformed plants, plant cells, seeds, and microorganisms comprising a nucleotide sequence that encodes an antipathogenic polypeptide of the invention are also disclosed.
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5.发明授权
公开(公告)号：EP0988383B1
公开(公告)日：2002-10-16
申请号：EP98933188.9
申请日：1998-07-07
发明人： DUVICK, Jonathan , MADDOX, Joyce, R. , ROOD, Tracy, A. , WANG, Xun , BOWEN, Benjamin, A. , GILLIAM, Jacob, T.
CPC分类号： A01N63/00 , C12N9/00 , C12N9/16 , C12N9/18 , C12N15/8209 , C12N15/8242 , C12N15/8282
摘要： Methods for identifying organisms capable of degrading fumonisin. Fumonisin can be incorporated into culture medium for selection of organisms resistant to fumonisin and/or capable of growing on fumonisin as a sole carbon source. Using this method, several organisms have been identified. These organisms can be used to isolate the enzymes and the genes responsible for conferring fumonisin-resistance. The gene can be cloned and inserted into a suitable expression vector so that the protein can be further characterized. Additionally, the DNA encoding for fumonisin degrading enzymes can be used to transform plant cells normally susceptible to Fusarium or other toxin-producing fungus infections. Plants can be regenerated from the transformed plant cells. In this way, a transgenic plant can be produced with the capability of degrading fumonisin, as well as with the capability of producing the degrading enzymes. Methods for detoxification in grain, grain processing, silage, food crops and in animal feed and rumen microbes are also disclosed.
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6.发明公开AMINO POLYOL AMINE OXIDASE POLYNUCLEOTIDES AND RELATED POLYPEPTIDES AND METHODS OF USE 审中-公开氨基多元醇胺氧化多核苷酸和相关的多肽和使用DeRene
公开(公告)号：EP1095151A1
公开(公告)日：2001-05-02
申请号：EP99933787.6
申请日：1999-07-08
CPC分类号： C12N9/0022 , A23K10/14 , A23K10/18 , A23K30/18 , C07K2319/00 , C12N9/16 , C12N15/8209 , C12N15/8242 , C12N15/8282 , G01N2333/906
摘要： The present invention provides polynucleotides and related polypeptides of the enzyme APAO isolated from Exophiala spinifera and Rhinocladiella atrovirens. Additionally, the polynucleotides encoding for the APAO enzyme can be used to transform plant cells normally susceptible to Fusarium or other toxin-producing fungus infection. Plants can be regenerated from the transformed plant cells. Additionally, the present invention provides for expressing both APAO and a fumonisin esterase in a transgenic plant. In this way, a transgenic plant can be produced with the capability of degrading fumonisin, as well as with the capability of producing the degrading enzymes. In addition, the present invention provides methods for producing the APAO enzyme in both prokaryotic and non-plant eukaryotic systems. Methods for detoxification in grain, grain processing, silage, food crops and in animal feed and rumen microbes are also disclosed.
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