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1.发明公开MAIZE CR1BIO GENE PROMOTER AND ITS USE TO DIRECT ROOT-PREFERRED TRANSGENE EXPRESSION IN PLANTS 审中-公开CR1BIO基因启动子的玉米及使用ROOT具体转基因的表达在植物中控制
公开(公告)号：EP1817420A2
公开(公告)日：2007-08-15
申请号：EP05825630.6
申请日：2005-11-15
发明人： DIEHN, Scott , LU, Albert, L. , SIMS, Lynne, E. , WARD, Kim, R.
CPC分类号： C12N15/8234 , C12N15/8227
摘要： The present invention provides compositions and methods for regulating expression of heterologous nucleotide sequences in a plant. Compositions include a novel nucleotide sequence for a root-preferred promoter for the gene encoding Cr1Bio. A method for expressing a heterologous nucleotide sequence in a plant using the promoter sequences disclosed herein is provided. The method comprises stabling incorporating into the genome of a plant cell a nucleotide sequence operably linked to the root-preferred promoter of the present invention and regenerating a stably transformed plant that expresses the nucleotide sequence.
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公开(公告)号：EP1133556A1
公开(公告)日：2001-09-19
申请号：EP99960418.4
申请日：1999-11-17
发明人： BRUCE, Wesley, B. , SIMS, Lynne, E.
CPC分类号： C12N15/8216 , C07K14/415 , C12N15/8222 , C12N15/8227
摘要： The present invention provides compositions and methods for regulating expression of heterologous nucleotide sequences in a plant. Compositions include a novel nucleotide sequence for a root-preferred promoter for the gene encoding Knox1, a Kn1-like homeobox gene and sequences isolated therefrom. A method for expressing a heterologous nucleotide sequence in a plant using the promoter sequences disclosed herein is provided. The method comprises stabling incorporating into the genome of a plant cell a nucleotide sequence operably linked to the root-preferred promoter of the present invention and regenerating a stably transformed plant that expresses the nucleotide sequence.
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公开(公告)号：EP1056875B1
公开(公告)日：2007-08-29
申请号：EP99908362.9
申请日：1999-02-23
CPC分类号： C12N9/0008 , C12N15/8216 , C12N15/8222 , C12N15/8225 , C12N15/8226 , C12N15/8279
摘要： Synthetic elements for enhancing expression of genes in plant cells are disclosed. These include a promoter with a 'TATA to start' sequence containing 64 % or greater GC content and a synthetic upstream element incorporating several OCS binding motifs and novel flanking sequences. Upstream activating regions (UARs) are also disclosed that can further increase the constitutive transcriptional activity when they are operably linked to said promoter and/or the synthetic upstream element. In particular, the nucleotide sequence of the UAR of the maize Ubi-1 gene is provided and its use in expression cassettes and vectors containing these promoter elements. Cells and plants transformed with these vectors are further provided. These include a transgenic sunflower expressing an exogenous oxalate oxidase gene at a high level under the transcriptional control of a recombinant promoter having at least one upstream activating region of the 35S CaMV promoter.
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4.发明公开PLANT URIDINE DIPHOSPHATE-GLUCOSE DEHYDROGENASE GENES, PROTEINS, AND USES THEREOF 审中-公开蔬菜尿苷二磷酸葡萄糖脱氢酶基因，蛋白质及其用途
公开(公告)号：EP1038005A2
公开(公告)日：2000-09-27
申请号：EP98965383.7
申请日：1998-12-09
发明人： NICHOLS, Scott, E. , DHUGGA, Kanwarpal, S. , SINGLETARY, George, W. , SAUNDERS, Court, A. , WITCHER, Derrick , BRUCE, Wesley, B. , SIMS, Lynne, E. , LU, Guihua , ZHONG, Gan-Yuan
IPC分类号： C12N15/53 , C12N15/82 , C12N5/10 , C12N9/04 , C08B30/00 , C08B30/04 , C08B31/00 , A01H5/00 , A23K1/00 , A23K1/14
CPC分类号： C12N9/0006 , A01K2217/05 , A23K10/30 , C12N15/8242 , C12N15/8245 , C12N15/8261 , C12N15/8286 , Y02A40/146 , Y02A40/162
摘要： Methods are provided that diminish UDPGdH expression in plant cells as compared to wild-type plant cells. These methods find use in increasing the nutritional value of animal feed and improving processes for extraction of starch from plant seeds. Also provided are expression vectors capable of diminishing UDPGdH expression in plant cells, and transgenic plants expressing diminished levels of UDPGdH.
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5.发明公开
公开(公告)号：EP0914454A1
公开(公告)日：1999-05-12
申请号：EP97929831.0
申请日：1997-06-10
发明人： BRUCE, Wesley, B. , SIMS, Lynne, E.
CPC分类号： C12N9/0008 , C12N15/8216 , C12N15/8222 , C12N15/8225 , C12N15/8226 , C12N15/8279
摘要： Synthetic elements for enhancing expression of genes in plant cells are disclosed. These include a promoter with a 'TATA to start' sequence containing 64 % or greater GC content and an upstream element incorporating several OCS binding motifs and novel flanking sequences.
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公开(公告)号：EP1133556B1
公开(公告)日：2006-02-08
申请号：EP99960418.4
申请日：1999-11-17
发明人： BRUCE, Wesley, B. , SIMS, Lynne, E.
CPC分类号： C12N15/8216 , C07K14/415 , C12N15/8222 , C12N15/8227
摘要： The present invention provides compositions and methods for regulating expression of heterologous nucleotide sequences in a plant. Compositions include a novel nucleotide sequence for a root-preferred promoter for the gene encoding Knox1, a Kn1-like homeobox gene and sequences isolated therefrom. A method for expressing a heterologous nucleotide sequence in a plant using the promoter sequences disclosed herein is provided. The method comprises stabling incorporating into the genome of a plant cell a nucleotide sequence operably linked to the root-preferred promoter of the present invention and regenerating a stably transformed plant that expresses the nucleotide sequence.
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7.发明授权
公开(公告)号：EP0914454B1
公开(公告)日：2005-03-09
申请号：EP97929831.2
申请日：1997-06-10
发明人： BRUCE, Wesley, B. , SIMS, Lynne, E.
CPC分类号： C12N9/0008 , C12N15/8216 , C12N15/8222 , C12N15/8225 , C12N15/8226 , C12N15/8279
摘要： Synthetic elements for enhancing expression of genes in plant cells are disclosed. These include a promoter with a 'TATA to start' sequence containing 64 % or greater GC content and an upstream element incorporating several OCS binding motifs and novel flanking sequences.
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公开(公告)号：EP1056875A1
公开(公告)日：2000-12-06
申请号：EP99908362.9
申请日：1999-02-23
CPC分类号： C12N9/0008 , C12N15/8216 , C12N15/8222 , C12N15/8225 , C12N15/8226 , C12N15/8279
摘要： Synthetic elements for enhancing expression of genes in plant cells are disclosed. These include a promoter with a 'TATA to start' sequence containing 64 % or greater GC content and a synthetic upstream element incorporating several OCS binding motifs and novel flanking sequences. Upstream activating regions (UARs) are also disclosed that can further increase the constitutive transcriptional activity when they are operably linked to said promoter and/or the synthetic upstream element. In particular, the nucleotide sequence of the UAR of the maize Ubi-1 gene is provided and its use in expression cassettes and vectors containing these promoter elements. Cells and plants transformed with these vectors are further provided. These include a transgenic sunflower expressing an exogenous oxalate oxidase gene at a high level under the transcriptional control of a recombinant promoter having at least one upstream activating region of the 35S CaMV promoter.
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