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    DNA sequences encoding biosynthetic insulin precursors and process for prepation of insulin
    1.
    发明公开
    DNA sequences encoding biosynthetic insulin precursors and process for prepation of insulin 失效
    Fürbiosynthetische Insulinvorstufen kodierende DNA-Sequenzen und Verfahren zur Herstellung von Insulin

    公开(公告)号：EP0704527A2

    公开(公告)日：1996-04-03

    申请号：EP95112210.0

    申请日：1995-08-03

    申请人： PLIVA, farmaceutska, kemijska, prehrambena i kozmeticka industrija dionicko drustvo

    发明人： Mestric, Silvija ,  Punt, Peter J. ,  Valinger, Radovan ,  van den Hondel, Cees A.M.J.J.

    IPC分类号： C12N15/17 ,  C12N1/15 ,  C12N15/62 ,  C07K14/62

    CPC分类号： C07K14/62 ,  A61K38/00 ,  C07K2319/00 ,  C12N15/80

    摘要： The present invention relates to DNA sequences encoding insulin precursors of formula B-Pg-A, wherein B and A represent B- and A-chains, respectively, and Pg represents modified C-peptide or any number of amino acids comprising at least one glycosylation consensus site. The invention also relates to a process for the preparation of insulin by culturing suitable cells, preferably fungal cells, transformed with a vector comprising DNA sequence encoding insulin precursor of given formula. The invention further relates to DNA sequences encoding pharmaceutical peptides and proteins in which N-glycosylation consensus site is introduced in a non-essential site in any spacer region for higher expression and secretion of proteins.

    摘要翻译： 本发明涉及编码式B-Pg-A的胰岛素前体的DNA序列，其中B和A分别代表B和A链，Pg代表修饰的C-肽或包含至少一个糖基化的任何数目的氨基酸 共识网站。 本发明还涉及通过培养由包含编码给定式胰岛素前体的DNA序列的载体转化的合适细胞，优选真菌细胞来制备胰岛素的方法。 本发明还涉及编码药物肽和蛋白质的DNA序列，其中N-糖基化共有位点被引入任何间隔区域中的非必需位点，用于蛋白质的更高表达和分泌。