公开(公告)号：EP1297337B1

公开(公告)日：2011-01-19

申请号：EP01942027.2

申请日：2001-06-07

申请人： PROMEGA CORPORATION

发明人： HAWKINS, Erika ,  CENTANNI, John, M. ,  SANKBEIL, Jacqueline ,  WOOD, Keith, V.

IPC分类号： G01N33/533

CPC分类号： C12Q1/66 ,  G01N33/582 ,  G01N2333/90241 ,  Y10S435/968 ,  Y10S435/975

摘要： A method for increasing the sensitivity of a luminescent assay comprising carrying out the assay in the presence of an organic compound that reduces luminescence that is not dependent on the presence of an analyte by at least about 10 fold, and that reduces luminescence that is dependent on the presence of an analyte by less than about 7 fold.

公开(公告)号：EP1179058A1

公开(公告)日：2002-02-13

申请号：EP99967755.2

申请日：1999-12-30

申请人： PROMEGA CORPORATION

发明人： BITNER, Rex, M. ,  SMITH, Craig, E. ,  SANKBEIL, Jacqueline ,  BUTLER, Braeden, L. ,  WHITE, Douglas, H.

IPC分类号： C12N15/10 ,  C12N5/00 ,  C12N1/02

CPC分类号： C12N5/00

摘要： Methods are disclosed for using paramagnetic particles to concentrate or harvest cells. Methods are also disclosed for clearing a solution of disrupted biological material, such as a lysate of cells or a homogenate of mammalian tissue. Methods are also disclosed for using paramagnetic particles to isolate target nucleic acids, such as RNA or DNA, from a solution cleared of disrupted biological material using the same type or a different type of paramagnetic particle. Kits are also disclosed for use with the various methods of the present invention. Nucleic acids isolated according to the present methods and using the present kits are suitable for immediate use in downstream processing, without further purification.

公开(公告)号：EP1179058B1

公开(公告)日：2011-08-24

申请号：EP99967755.2

申请日：1999-12-30

申请人： PROMEGA CORPORATION

发明人： BITNER, Rex, M. ,  SMITH, Craig, E. ,  SANKBEIL, Jacqueline ,  BUTLER, Braeden, L. ,  WHITE, Douglas, H.

IPC分类号： C12N15/10 ,  C12N5/00 ,  C12N1/02

CPC分类号： C12N5/00

摘要： Methods are disclosed for using paramagnetic particles to concentrate or harvest cells. Methods are also disclosed for clearing a solution of disrupted biological material, such as a lysate of cells or a homogenate of mammalian tissue. Methods are also disclosed for using paramagnetic particles to isolate target nucleic acids, such as RNA or DNA, from a solution cleared of disrupted biological material using the same type or a different type of paramagnetic particle. Kits are also disclosed for use with the various methods of the present invention. Nucleic acids isolated according to the present methods and using the present kits are suitable for immediate use in downstream processing, without further purification.

公开(公告)号：EP1297337A2

公开(公告)日：2003-04-02

申请号：EP01942027.2

申请日：2001-06-07

申请人： PROMEGA CORPORATION

发明人： HAWKINS, Erika ,  CENTANNI, John, M. ,  SANKBEIL, Jacqueline ,  WOOD, Keith, V.

IPC分类号： G01N33/533

CPC分类号： C12Q1/66 ,  G01N33/582 ,  G01N2333/90241 ,  Y10S435/968 ,  Y10S435/975

摘要： A method for increasing the sensitivity of a luminescent assay comprising carrying out the assay in the presence of an organic compound that reduces luminescence that is not dependent on the presence of an analyte by at least about 10 fold, and that reduces luminescence that is dependent on the presence of an analyte by less than about 7 fold.