公开(公告)号：EP1179058A1

公开(公告)日：2002-02-13

申请号：EP99967755.2

申请日：1999-12-30

申请人： PROMEGA CORPORATION

发明人： BITNER, Rex, M. ,  SMITH, Craig, E. ,  SANKBEIL, Jacqueline ,  BUTLER, Braeden, L. ,  WHITE, Douglas, H.

IPC分类号： C12N15/10 ,  C12N5/00 ,  C12N1/02

CPC分类号： C12N5/00

摘要： Methods are disclosed for using paramagnetic particles to concentrate or harvest cells. Methods are also disclosed for clearing a solution of disrupted biological material, such as a lysate of cells or a homogenate of mammalian tissue. Methods are also disclosed for using paramagnetic particles to isolate target nucleic acids, such as RNA or DNA, from a solution cleared of disrupted biological material using the same type or a different type of paramagnetic particle. Kits are also disclosed for use with the various methods of the present invention. Nucleic acids isolated according to the present methods and using the present kits are suitable for immediate use in downstream processing, without further purification.

公开(公告)号：EP1179058B1

公开(公告)日：2011-08-24

申请号：EP99967755.2

申请日：1999-12-30

申请人： PROMEGA CORPORATION

发明人： BITNER, Rex, M. ,  SMITH, Craig, E. ,  SANKBEIL, Jacqueline ,  BUTLER, Braeden, L. ,  WHITE, Douglas, H.

IPC分类号： C12N15/10 ,  C12N5/00 ,  C12N1/02

CPC分类号： C12N5/00

摘要： Methods are disclosed for using paramagnetic particles to concentrate or harvest cells. Methods are also disclosed for clearing a solution of disrupted biological material, such as a lysate of cells or a homogenate of mammalian tissue. Methods are also disclosed for using paramagnetic particles to isolate target nucleic acids, such as RNA or DNA, from a solution cleared of disrupted biological material using the same type or a different type of paramagnetic particle. Kits are also disclosed for use with the various methods of the present invention. Nucleic acids isolated according to the present methods and using the present kits are suitable for immediate use in downstream processing, without further purification.

公开(公告)号：EP1907585A2

公开(公告)日：2008-04-09

申请号：EP06785973.6

申请日：2006-06-30

申请人： PROMEGA CORPORATION

发明人： BITNER, Rex, M. ,  MANDREKAR, Michelle ,  SMITH, Don ,  WHITE, Douglas, H.

IPC分类号： C12Q1/68 ,  C12Q1/00

CPC分类号： C12N15/1006 ,  C12Q1/6806 ,  C12Q2563/149

摘要： A network of buoyant particles for clearing lysates of biological material, the network including two or more buoyant particles covalently linked together, wherein the network ranges in size from approximately 30 microns to approximately one centimeter along the network's longest dimension. The buoyant particles may have a silica surface. The network may have a density less than about 1.2 g/cm3. Methods of making the network of buoyant particles and methods of isolating target biological material using buoyant particles or a network of buoyant particles are also described.

公开(公告)号：EP1071695A1

公开(公告)日：2001-01-31

申请号：EP99918650.5

申请日：1999-04-22

申请人： PROMEGA CORPORATION

发明人： SMITH, Craig, E. ,  CRESWELL, Donald, A. ,  BITNER, Rex, M. ,  WHITE, Douglas, H. ,  BUTLER, Braeden, L. ,  LESLEY, Scott, A.

IPC分类号： C07H21/00 ,  C07H21/02 ,  C07H21/04 ,  C12Q1/68

CPC分类号： C12N15/1013 ,  C12N15/1006

摘要： The present invention presents a novel method for removing endotoxins from nucleic acids, such as DNA, RNA, or hybrids thereof, contaminated therewith. Nucleic acid solutions which can be treated using the method of this invention include, but are not limited to, lysates of gram-negative bacteria and nucleic acid solutions contaminated with endotoxins from external sources. The present method removes endotoxins from such solutions using silica-based materials, such as silica gel particles, magnetic silica particles, or diatomaceous earth. In a preferred aspect of the method of this invention, magnetic silica particles are used to isolate plasmid DNA from a lysate of gram-negative bacteria transformed with the plasmid DNA. Application of the disclosed method produces nucleic acids which are sufficiently free of endotoxin contamination to be useful for a variety of different practical applications. Figure 1 shows a plot of units of endotoxin bound vs the number of milligrams of particles added to lysates of each of two differerent strains of bacteria, JM109 (◊) or DH5α(♦).

公开(公告)号：EP0646242A1

公开(公告)日：1995-04-05

申请号：EP92925108.0

申请日：1992-11-12

申请人： PROMEGA CORPORATION

发明人： SHULTZ, John, W. ,  WHITE, Douglas, H.

IPC分类号： C07K7 ,  C12Q1 ,  G01N33

CPC分类号： C07K7/06 ,  C07K7/08 ,  C12Q1/37 ,  C12Q1/42 ,  C12Q1/48 ,  Y10S435/81 ,  Y10S435/975 ,  Y10S436/80 ,  Y10T436/103332 ,  Y10T436/104165 ,  Y10T436/104998 ,  Y10T436/105831 ,  Y10T436/106664 ,  Y10T436/107497

摘要： The present invention is directed to the assay and purification of proteins, and particularly to the non-radioactive assay and purification of protein kinases, phosphatases and protease by incubating the enzyme with a substrate modified peptide to form a product modified peptide under conditions where the enzyme is active. The product modified peptide and substrate modified peptide are then separated, and the product modified peptide is measured. The present invention is also directed to kits and bioreagents for performing the assays.