公开(公告)号：EP0555440A4

公开(公告)日：1994-03-23

申请号：EP92917839

申请日：1992-07-30

申请人： PSYCHEMEDICS CORPORATION

发明人： BAUMGARTNER, WERNER, A.

IPC分类号： G01N1/28 ,  C12Q1/37 ,  G01N1/00 ,  G01N1/34 ,  G01N1/40 ,  G01N33/48 ,  G01N33/53 ,  G01N33/531 ,  G01N33/94

CPC分类号： C12Q1/37 ,  G01N1/4044 ,  G01N1/405 ,  G01N33/53 ,  G01N33/94 ,  G01N33/948 ,  G01N2001/007 ,  G01N2333/4742 ,  G01N2333/96433 ,  Y10S436/816 ,  Y10S436/825 ,  Y10S436/901 ,  Y10T436/25125 ,  Y10T436/25375

摘要： A method the direct analysis of an analyte in keratinized structures, e.g., hair and fingernails, which comprises preparing a mixture containing a low redox potential activator compound such as dithiothreitol or dithioerythritol, an enzyme suitable for the digestion of the keratin structure, a sample of the keratin structure and a biological detergent that aids the digestion of the keratinized structure at a relatively low pH, e.g., between about 6.2 and 8; permitting the enzyme to at least substantially digest the sample of keratin structure, and subjecting the digest solution to analysis, preferably by radioimmunoassay, to determine the identity and amount of analyte in the keratin structure sample. To accelerate the method, cupric sulfate may be added to the mixture after degradation of the keratin sample. The enzyme may be a peptidase, endopeptidase or proteinase, with papain, chymopapain, and proteinase K being preferred for use in the invention. The preferred biological detergents include betaine, sulfo-betaine, alkylglucosides and bile acids.

摘要翻译： 一种用于直接分析角质化结构例如毛发和指甲中分析物的方法，其包括制备含有低氧化还原电位活化剂化合物如二硫苏糖醇或二硫赤藓糖醇的混合物，适合于消化角蛋白结构的酶，样品 角蛋白结构和生物洗涤剂，所述生物洗涤剂有助于角蛋白化结构在相对低的pH例如约6.2至8之间的消化; 允许酶至少基本上消化角蛋白结构样品，并且优选通过放射免疫测定法对消化溶液进行分析以确定角蛋白结构样品中分析物的身份和量。 为了加速该方法，可以在角蛋白样品降解后将硫酸铜添加到混合物中。 酶可以是肽酶，内肽酶或蛋白酶，木瓜蛋白酶，木瓜凝乳蛋白酶和蛋白酶K优选用于本发明。 优选的生物洗涤剂包括甜菜碱，磺基甜菜碱，烷基葡糖苷和胆汁酸。