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公开(公告)号：EP2281204A1
公开(公告)日：2011-02-09
申请号：EP09739657.6
申请日：2009-04-29
IPC分类号： G01N33/94
CPC分类号： G01N33/94
摘要： Compositions and methods for detecting the presence and/or amount of one or more analytes, including analytes such as drugs of abuse, are provided. The compositions include two or more analytes associated with a solid phase, e.g., a particle or a multiwell plate. The compositions and methods also allow the simultaneous, tandem, or serial determination of the presence and/or amount of two or more analytes of interest in a sample.
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公开(公告)号：EP0634014A1
公开(公告)日：1995-01-18
申请号：EP94907938.0
申请日：1994-02-01
CPC分类号： G01N1/405 , C12Q1/37 , G01N1/4044 , G01N33/53 , G01N33/94 , G01N33/948 , G01N2001/007 , G01N2333/4742 , G01N2333/96433 , Y10S435/961 , Y10S435/962 , Y10S435/963 , Y10S436/815 , Y10S436/825 , Y10S436/901 , Y10T436/25125 , Y10T436/25375
摘要： A method for the direct analysis of analyte indicative of marijuana exposure found in keratinized structures, e.g., hair, fingernails and toenails, which comprises preparing a mixture containing dithiothreitol or dithioerythritol, a protease suitable for the digestion of the keratin structure and a sample of the keratin structure; permitting the enzyme to at least substantially digest the sample of keratin structure to form a digest solution, followed by mixing the digest solution with a suspension of an ion exchange resin to remove an interfering, cross reacting substance naturally found in hair and finally subjecting the digest solution to analysis to determine the identity and amount of marijuana analyte in the keratin structure sample. To accelerate the method, cupric sulfate may be added to the mixture after degradation of the keratin sample in order to deactivate the activator. The enzyme may be a protease with papain, chymopapain, and proteinase K being preferred for use in the invention. Exemplary ion exchange resins useful in the method according to the invention are DEAE Sephadex (Diethylaminoethyl Sephadex) and DEAE Sepharose (Diethylaminoethyl Sepharose).
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公开(公告)号：EP0555440A1
公开(公告)日：1993-08-18
申请号：EP92917839.0
申请日：1992-07-30
CPC分类号： C12Q1/37 , G01N1/4044 , G01N1/405 , G01N33/53 , G01N33/94 , G01N33/948 , G01N2001/007 , G01N2333/4742 , G01N2333/96433 , Y10S436/816 , Y10S436/825 , Y10S436/901 , Y10T436/25125 , Y10T436/25375
摘要： Une méthode d'analyse directe d'un analyte dans des structures kératinisées, par exemple les cheveux et les ongles, consiste à préparer un mélange contenant un composé activateur à faible potentiel redox tel que le dithiothréitol ou le dithioérythritol, une enzyme appropriée pour la digestion de la structure de kératine, un échantillon de la structure de kératine et un détergent biologique qui facilite la digestion de la structure kératinisée à un pH relativement faible, par exemple entre 6,2 et 8; on laisse ensuite l'enzyme digérer l'échantillon de la structure de kératine, et on soumet la solution de digestion à une analyse, de préférence par radio immuno-analyse afin de déterminer l'identité et la quantité de l'analyte dans l'échantillon de la structure de kératine. Pour accélérer le procédé, on peut ajouter le sulfate de cuivre au mélange après dégradation de l'échantillon de kératine. L'enzyme peut être une peptidase, un endopeptidase ou une protéinase, les enzymes utilisées de préférence dans l'invention étant la papaïne, la chimiopapaïne et la protéinase K. Les détergents biologiques préférés comprennent la bétaïne, la sulfo-bétaïne les alkylglucosides et les acides biliaires.
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公开(公告)号：EP3039432A2
公开(公告)日：2016-07-06
申请号：EP14761936.5
申请日：2014-08-28
申请人： Psychemedics Corporation , Hill, Virginia , Schaffer, Michael I. , Loni, Elvan , Stowe, Gary Neil
IPC分类号： G01N33/94
CPC分类号： G01N33/946
摘要： Methods for assessing the condition of keratinized structures, including hair, in particular methods to determine the condition of keratinized structures in relation to suitability for analysis of analytes of interest in a test sample, are presented. The methods comprise contacting the keratinized structure with a non-proteolytic reducing agent and an optional proteolytic agent. The methods further include inspection of the hair sample, or measurement of free protein eluted from the keratinized structure, after reduction and optional proteolysis to determine condition prior to analyte identification and quantitation by known techniques such as immunoassays.
摘要翻译： 提供了评估包括头发在内的角化结构的状况的方法，特别是确定角质化结构的条件与测试样品中感兴趣的分析物的适用性相关的方法。 所述方法包括使角质化结构与非蛋白水解还原剂和任选的蛋白水解剂接触。 所述方法还包括检查毛发样品，或测量从角质化结构洗脱的游离蛋白，还原和任选的蛋白水解后，通过已知技术例如免疫测定来确定分析物鉴定和定量之前的状态。
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公开(公告)号：EP0555440A4
公开(公告)日：1994-03-23
申请号：EP92917839
申请日：1992-07-30
IPC分类号： G01N1/28 , C12Q1/37 , G01N1/00 , G01N1/34 , G01N1/40 , G01N33/48 , G01N33/53 , G01N33/531 , G01N33/94
CPC分类号： C12Q1/37 , G01N1/4044 , G01N1/405 , G01N33/53 , G01N33/94 , G01N33/948 , G01N2001/007 , G01N2333/4742 , G01N2333/96433 , Y10S436/816 , Y10S436/825 , Y10S436/901 , Y10T436/25125 , Y10T436/25375
摘要： A method the direct analysis of an analyte in keratinized structures, e.g., hair and fingernails, which comprises preparing a mixture containing a low redox potential activator compound such as dithiothreitol or dithioerythritol, an enzyme suitable for the digestion of the keratin structure, a sample of the keratin structure and a biological detergent that aids the digestion of the keratinized structure at a relatively low pH, e.g., between about 6.2 and 8; permitting the enzyme to at least substantially digest the sample of keratin structure, and subjecting the digest solution to analysis, preferably by radioimmunoassay, to determine the identity and amount of analyte in the keratin structure sample. To accelerate the method, cupric sulfate may be added to the mixture after degradation of the keratin sample. The enzyme may be a peptidase, endopeptidase or proteinase, with papain, chymopapain, and proteinase K being preferred for use in the invention. The preferred biological detergents include betaine, sulfo-betaine, alkylglucosides and bile acids.
摘要翻译： 一种用于直接分析角质化结构例如毛发和指甲中分析物的方法，其包括制备含有低氧化还原电位活化剂化合物如二硫苏糖醇或二硫赤藓糖醇的混合物，适合于消化角蛋白结构的酶，样品 角蛋白结构和生物洗涤剂，所述生物洗涤剂有助于角蛋白化结构在相对低的pH例如约6.2至8之间的消化; 允许酶至少基本上消化角蛋白结构样品，并且优选通过放射免疫测定法对消化溶液进行分析以确定角蛋白结构样品中分析物的身份和量。 为了加速该方法，可以在角蛋白样品降解后将硫酸铜添加到混合物中。 酶可以是肽酶，内肽酶或蛋白酶，木瓜蛋白酶，木瓜凝乳蛋白酶和蛋白酶K优选用于本发明。 优选的生物洗涤剂包括甜菜碱，磺基甜菜碱，烷基葡糖苷和胆汁酸。
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公开(公告)号：EP0458594A3
公开(公告)日：1992-10-07
申请号：EP91304587.8
申请日：1991-05-21
IPC分类号： G01N33/94
CPC分类号： G01N33/94 , C12Q1/37 , G01N2333/4742 , G01N2333/96466
摘要： A method for the direct analysis of analyte in keratinized structures, e.g., hair, fingernails and toenails, which comprises preparing a mixture containing a low redox potential compound such as dithiothreitol or dithioerythritol, an enzyme suitable for the degradation of the keratine structure and a sample of the keratin structure; permitting the enzyme to at least substantially degrade the sample of keratin structure, and subjecting the mixture to analysis to determine the identity and amount of analyte in the keratin substance sample. To accelerate the method, cupric sulfate or sodium arsenite may be added to the mixture after degradation of the keratin sample. The enzyme may be a peptidase, endopeptidase or proteinase, with papain, chymopapain, and proteinase K being preferred for use in the invention.
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8.发明授权Non-proteolytic method for the determination of analytes in keratinized structures 有权用于分析物的角化结构中判定非蛋白水解方法
公开(公告)号：EP2637026B1
公开(公告)日：2015-09-30
申请号：EP13170392.8
申请日：2009-04-29
IPC分类号： G01N33/94
CPC分类号： G01N33/9486 , Y10S435/962 , Y10T436/10
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9.发明授权
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10.发明授权NON-PROTEOLYTIC METHOD FOR THE DETERMINATION OF ANALYTES IN KERATINIZED STRUCTURES 有权FOR角化结构分析物厘定非蛋白水解法
公开(公告)号：EP2283367B1
公开(公告)日：2013-06-12
申请号：EP09739654.3
申请日：2009-04-29
IPC分类号： G01N33/94
CPC分类号： G01N33/9486 , Y10S435/962 , Y10T436/10
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