公开(公告)号：EP2227564A2

公开(公告)日：2010-09-15

申请号：EP08859169.8

申请日：2008-11-24

申请人： Perkinelmer LAS, Inc.

发明人： ADLER, Karl, Edwin ,  SCHERMER, Mack, J.

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6811 ,  C12Q1/6809 ,  C12Q1/6813 ,  C12Q1/6876 ,  Y10T436/143333 ,  C12Q2563/149 ,  C12Q2537/143 ,  C12Q2531/113 ,  C12Q2525/179 ,  C12Q2539/115

摘要： Encoded bead multiplex assays for chromosomal gains and losses are provided that provide the benefits of complex, large template DNA sources, such as BAC DNA, as the probe material without bead networking or other assay performance problems. Reagents for assaying DNA are described herein which include a plurality of encoded particles having attached amplicons amplified from a template DNA sequence. Each individual attached amplicon includes a nucleic acid sequence identical to a random portion of the template DNA sequence, wherein the amplicons together represent substantially the entire template DNA and wherein the nucleic acid sequence identical to a random portion of the template DNA sequence of each individual amplicon is shorter than the entire template DNA.

公开(公告)号：EP1969372A2

公开(公告)日：2008-09-17

申请号：EP06847930.2

申请日：2006-12-22

申请人： PerkinElmer LAS, Inc.

发明人： BOBROW, Mark, N. ,  ADLER, Karl, Edwin, Jr. ,  SCHERMER, Mack, J.

IPC分类号： G01N33/53 ,  G01N33/573 ,  G01N33/542

CPC分类号： C12Q1/34 ,  C12Q1/00 ,  G01N33/585 ,  G01N2333/98 ,  G01N2800/04

摘要： This invention relates to methods and compositions useful in detecting enzymatic activity. Also featured are methods for diagnosing enzyme deficiencies such as metabolic disorders.

公开(公告)号：EP1869219A2

公开(公告)日：2007-12-26

申请号：EP06749037.5

申请日：2006-03-31

申请人： PerkinElmer LAS, Inc.

发明人： BOBROW, Mark, N. ,  SCHERMER, Mack, J.

IPC分类号： C12Q1/68 ,  C12M1/34

CPC分类号： C12Q1/6818 ,  C12Q1/6834 ,  G01N33/585 ,  C12Q2565/537 ,  C12Q2563/149 ,  C12Q2563/103 ,  C12Q2565/1015 ,  C12Q2565/525 ,  C12Q2537/143 ,  C12Q2525/301 ,  C12Q2565/101 ,  C12Q2525/186 ,  C12Q2525/161 ,  C12Q2537/1373 ,  C12Q2533/101

摘要： The methods described herein can be used, e.g., for detecting at least two target molecules in a sample (e.g., a single sample). Exemplary methods include using a target-specific switch probe attached to a substrate and using zip codes to associate holographically encoded particles with a target-specific probe.

公开(公告)号：EP1740284A2

公开(公告)日：2007-01-10

申请号：EP05725938.4

申请日：2005-03-18

申请人： PerkinElmer LAS, Inc.

发明人： PATTON, Wayne, F. ,  SCHERMER, Mack, J.

IPC分类号： B01D15/08

CPC分类号： C07K1/26 ,  C07K1/285 ,  G01N27/44747 ,  G01N30/58 ,  G01N30/92 ,  G01N2030/8813 ,  G01N2030/945

摘要： The present invention describes a system and method for separation of proteins, peptides and glycans by one-dimensional or two-dimensional electroosmosis-driven planar chromatography. Separation is performed using amphiphilic polymeric membranes, amphiphilic thin-layer chromatography plates or other planar amphiphilic surfaces as the stationary phase with a combination of organic and/or aqueous buffers as the mobile phase. Systematic selection of stationary phase supports, mobile phase buffers and operating conditions allow for the adaptation of the invention to a broad range of applications in proteomics, mass spectrometry, drug discovery and the pharmaceutical sciences.