公开(公告)号：EP1743034B1

公开(公告)日：2007-12-12

申请号：EP05769759.1

申请日：2005-05-04

申请人： Pharmaleads

发明人： FOURNIE-ZALUSKI, Marie-Claude ,  ROQUES, Bernard, Pierre

IPC分类号： C12Q1/37

CPC分类号： C07K14/705 ,  C07K5/0812 ,  C07K5/1016 ,  C12Q1/37 ,  G01N2333/96486

摘要： The invention relates to a peptide substrate selectively recognisable by a botulinum toxin A, BoNT/A comprising a Nop-(Z)-Pya fragment in the peptide structure thereof, wherein Z is an aminoacid chain, preferably RA and said fragment is cleaved by the toxin. Said invention also relates to the substrate use, in particular for carrying out methods for detecting, identifying and/or dosing the botulinum toxin A at very low concentrations (of order of 20 pg) or the inhibitors and/or activators of the toxin metallopeptidase activity, thereby making it possible to quantify the power thereof.

摘要翻译： 本发明涉及可由肉毒杆菌毒素A选择性识别的肽底物，在其肽结构中包含Nop-（Z）-Pya片段的BoNT / A，其中Z是氨基酸链，优选RA，并且所述片段被 毒素。 所述发明还涉及底物用途，特别是用于实施用于检测，鉴定和/或定量施用非常低浓度（20pg左右）的肉毒杆菌毒素A或毒素金属肽酶活性的抑制剂和/或活化剂的方法 从而可以量化其功率。

公开(公告)号：EP1743034A2

公开(公告)日：2007-01-17

申请号：EP05769759.1

申请日：2005-05-04

申请人： Pharmaleads

发明人： FOURNIE-ZALUSKI, Marie-Claude ,  ROQUES, Bernard, Pierre

IPC分类号： C12Q1/37

CPC分类号： C07K14/705 ,  C07K5/0812 ,  C07K5/1016 ,  C12Q1/37 ,  G01N2333/96486

摘要： The invention relates to a peptide substrate selectively recognisable by a botulinum toxin A, BoNT/A comprising a Nop-(Z)-Pya fragment in the peptide structure thereof, wherein Z is an aminoacid chain, preferably RA and said fragment is cleaved by the toxin. Said invention also relates to the substrate use, in particular for carrying out methods for detecting, identifying and/or dosing the botulinum toxin A at very low concentrations (of order of 20 pg) or the inhibitors and/or activators of the toxin metallopeptidase activity, thereby making it possible to quantify the power thereof.