公开(公告)号：EP2751273B1

公开(公告)日：2018-07-04

申请号：EP12758405.0

申请日：2012-08-31

申请人： Philip Morris Products S.A.

发明人： BOVET, Lucien ,  SIERRO, Nicolas

IPC分类号： C12N15/82 ,  C12N9/88 ,  A01H5/00

CPC分类号： C12N9/88 ,  A24B13/00 ,  C07C381/00 ,  C12N15/8253 ,  C12Q1/6895 ,  C12Q2600/13 ,  C12Q2600/16 ,  C12Y402/03001

摘要： There is disclosed a mutant, non-naturally occurring or transgenic plant cell comprising: (i) a polynucleotide comprising, consisting or consisting essentially of a sequence encoding a threonine synthase and having at least 90% sequence identity to SEQ ID NO:1, SEQ ID NO:2 or SEQ ID NO:3 or at least 87% sequence identity to SEQ ID NO:4, or SEQ ID NO:5; (ii) a polypeptide encoded by any one of said polynucleotides set forth in (i); or (iii) a polypeptide having at least 95% sequence identity to SEQ ID NO:6, SEQ ID NO:7 or SEQ ID NO:8; or (iv) a construct, vector or expression vector comprising the polynucleotide as set forth in (i).

公开(公告)号：EP2751273A1

公开(公告)日：2014-07-09

申请号：EP12758405.0

申请日：2012-08-31

申请人： Philip Morris Products S.A.

发明人： BOVET, Lucien ,  SIERRO, Nicolas

IPC分类号： C12N15/82 ,  C12N9/88 ,  A01H5/00

CPC分类号： C12N9/88 ,  A24B13/00 ,  C07C381/00 ,  C12N15/8253 ,  C12Q1/6895 ,  C12Q2600/13 ,  C12Q2600/16 ,  C12Y402/03001

摘要： There is disclosed a mutant, non-naturally occurring or transgenic plant cell comprising: (i) a polynucleotide comprising, consisting or consisting essentially of a sequence encoding a threonine synthase and having at least 90% sequence identity to SEQ ID NO:1, SEQ ID NO:2 or SEQ ID NO:3 or at least 87% sequence identity to SEQ ID NO:4, or SEQ ID NO:5; (ii) a polypeptide encoded by any one of said polynucleotides set forth in (i); or (iii) a polypeptide having at least 95% sequence identity to SEQ ID NO:6, SEQ ID NO:7 or SEQ ID NO:8; or (iv) a construct, vector or expression vector comprising the polynucleotide as set forth in (i).

摘要翻译： 公开了一种突变体，非天然存在或转基因植物细胞，其包含：（i）多核苷酸，其包含，由SEQ ID NO：1，SEQ ID NO：1，SEQ ID NO：1，SEQ ID NO：1至少90％的序列同一性编码苏氨酸合成酶 SEQ ID NO：2或SEQ ID NO：3或至少87％与SEQ ID NO：4或SEQ ID NO：5的序列同一性; （ii）由（i）中所述的任一种所述多核苷酸编码的多肽; 或（iii）与SEQ ID NO：6，SEQ ID NO：7或SEQ ID NO：8具有至少95％序列同一性的多肽; 或（iv）包含如（i）所述的多核苷酸的构建体，载体或表达载体。

公开(公告)号：EP3395948A1

公开(公告)日：2018-10-31

申请号：EP18177740.0

申请日：2012-08-31

申请人： Philip Morris Products S.A.

发明人： BOVET, Lucien ,  SIERRO, Nicolas

IPC分类号： C12N15/82 ,  C12N9/88 ,  A01H5/00

CPC分类号： C12N9/88 ,  A24B13/00 ,  C07C381/00 ,  C12N15/8253 ,  C12Q1/6895 ,  C12Q2600/13 ,  C12Q2600/16 ,  C12Y402/03001

摘要： A method for increasing the concentration of free methionine in at least a part of a tobacco plant, comprising the steps of:
(a) reducing the expression or activity of threonine synthase in the tobacco plant, wherein the threonine synthase comprises
(i) a polynucleotide comprising or consisting of a sequence encoding a threonine synthase and having at least 90% sequence identity to SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, or SEQ ID NO:5;
(ii) a polypeptide encoded by any one of said polynucleotides set forth in (i); or
(iii) a polypeptide having at least 95% sequence identity to SEQ ID NO:6, SEQ ID NO:7 or SEQ ID NO:8;

(b) measuring the concentration of free methionine in at least a part of the mutant or non-naturally occurring plant obtained in step (i); and
(c) identifying a mutant or non-naturally occurring tobacco plant in which the concentration of free methionine therein has increased by at least 50% in comparison to a control tobacco plant in which the expression or activity of threonine synthase has not been reduced and wherein the visual appearance of said mutant or non-naturally occurring tobacco plant is substantially the same as the control tobacco plant three months after field transplant or 36 days after topping.

公开(公告)号：EP3140404A1

公开(公告)日：2017-03-15

申请号：EP15726007.6

申请日：2015-05-07

申请人： Philip Morris Products S.A.

发明人： LIEDSCHULTE, Verena ,  GOEPFERT, Simon ,  BOVET, Lucien ,  SIERRO, Nicolas

IPC分类号： C12N15/82 ,  C12N9/02 ,  A01H5/00

CPC分类号： C12N15/8243 ,  C12N9/0073 ,  C12N15/8218 ,  C12Q1/6895 ,  C12Q2600/13 ,  C12Q2600/156 ,  C12Y114/13

摘要： The present invention relates to a mutant, non-naturally occurring or transgenic tobacco plant cell comprising: (i) a polynucleotide comprising, consisting or consisting essentially of a sequence encoding a functional nicotine N-demethylase and having at least 95% sequence identity to SEQ ID NO:2; (ii) a polypeptide encoded by the polynucleotide set forth in (i); (iii) a polypeptide comprising, consisting or consisting essentially of a sequence encoding a nicotine N-demethylase and having at least 95% sequence identity to SEQ ID NO:3; or (iv) a construct, vector or expression vector comprising the isolated polynucleotide set forth in (i), and wherein the expression or activity of said nicotine demethylase is reduced as compared to a control tobacco plant cell in which the expression or activity of said nicotine demethylase has not been reduced.

摘要翻译： 本发明涉及一种突变体，非天然存在或转基因烟草植物细胞，其包含：（i）多核苷酸，其包含，组成或者基本上由编码功能性尼古丁N-脱甲基酶的序列组成，并且与SEQ ID不得少于95％的序列同一性 编号：2; （ii）由（i）所述的多核苷酸编码的多肽; （iii）一种多肽，其包含由编码尼古丁N-脱甲基酶并与SEQ ID NO：3具有至少95％序列同一性的序列组成或由其组成; 或者（iv）包含（i）所述的分离的多核苷酸的构建体，载体或表达载体，并且其中所述尼古丁脱甲基酶的表达或活性与所述烟碱脱甲基酶的表达或活性相比降低， 尼古丁脱甲基酶尚未减少。