摘要:
The present invention relates to a method for manufacturing enantiomerically pure (13R)-manoyl oxide, said method comprising the steps of contacting geranylgeranyl diphosphate (GGPP) with a class II diterpene synthase to obtain labd-13-en-8,15-diol diphosphate (LPP), and then contacting the LPP with a class I diterpene synthase to obtain (13R)-manoyl oxide. The invention further relates to (13R)-manoyl oxide obtained by the method of the invention.
摘要:
The present invention relates to modifications of berberine bridge enzyme-like nucleic acids and their use in modulation of nicotine biosynthesis in plants.
摘要:
Provided is a vector capable of improving polyisoprenoid production through the introduction of the vector into a plant using gene recombination techniques. A vector comprising: a promoter of a gene encoding Small Rubber Particle Protein; and a gene encoding a protein involved in polyisoprenoid biosynthesis, the gene being functionally linked to the promoter.
摘要:
A Papaver plant species modified in one or more genes encoding a codeine 3-O-demethylase (CODM) wherein the modification reduces the expression or activity of the enzyme and wherein the modified plant has elevated codeine content when compared to a wild-type Papaver plant. Also claimed are mutated codeine 3-O-demethylase gene sequences.
摘要:
The present disclosure provides the identification of tobacco Nic1 locus. Also provided are tobacco plants with altered total alkaloid and nicotine levels and commercially acceptable leaf grade, their development via breeding or transgenic approaches, and production of tobacco products from these tobacco plants. Also provided are compositions and methods for producing tobacco plants having novel Nic1 mutations or alleles to reduce nicotine levels. Further provided are sequence polymorphisms and molecular markers for breeding tobacco with reduced nicotine or nicotine free while maintaining tobacco leaf grade and tobacco product quality.
摘要:
The present invention provides an expression cassette comprising at least one polynucleotide comprising a nucleotide sequence selected from the group consisting of: a) a nucleotide sequence that encodes a polypeptide having the amino acid sequence set forth in SEQ ID NOs. 13-24; and b) a nucleic acid sequence that encodes a polypeptide having the amino acid sequence sharing at least 50%, preferably at least 55%, 60%, 65%, 70%, 75%, 80%, 85%, especially preferably at least 90%, 95%, 97%, 98%, 99%, or more of sequence identity with a sequence selected in the group consisting of SEQ ID NOs. 13-24. This expression cassette can be used in vivo to increase in plants the tolerance to abiotic stress, such as e.g., sulphur deficiency, nitrogen deficiency, or carbon/nitrogen imbalance. In another aspect, the invention thus relates to a transgenic plant cell comprising the expression cassette described above.
摘要:
The invention relates to isolated nucleic acids encoding a feruloyl-CoA:monolignol transferase and feruloyl-CoA:monolignol transferase enzymes. The isolated nucleic acids and/or the enzymes enable incorporation of monolignol ferulates into the lignin of plants, where such monolignol ferulates include, for example, p-coumaryl ferulate, coniferyl ferulate, and/or sinapyl ferulate. The invention also includes methods and plants that include nucleic acids encoding a feruloyl-CoA:monolignol transferase enzyme and/or feruloyl-CoA:monolignol transferase enzymes.
摘要:
Polypeptides having alkanoyl-CoA activity have been identified and characterized, as have nucleic acids encoding these polypeptides. Expression or over-expression of the nucleic acids alters levels of cannabinoid compounds in organisms. The polypeptides may be used in vivo or in vitro to produce cannabinoid compounds.
摘要:
The invention provides a nucleic acid encoding an enzyme capable of producing 9-hydroxy-7-epi-zingiberene and 10,11-epoxy-9-hydroxy-7-epizingiberene from 7-epi-zingiberene. The invention further provides novel zingiberene derivatives that can be used for increasing pest resistance of plants.