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    REDUCTION OF NICOTINE TO NORNICOTINE CONVERSION IN PLANTS
    3.
    发明公开
    REDUCTION OF NICOTINE TO NORNICOTINE CONVERSION IN PLANTS 审中-公开
    PFLANZEN VON NIKOTINFÜRDIE NORNICOTINUMWANDLUNG

    公开(公告)号：EP3140404A1

    公开(公告)日：2017-03-15

    申请号：EP15726007.6

    申请日：2015-05-07

    申请人： Philip Morris Products S.A.

    发明人： LIEDSCHULTE, Verena ,  GOEPFERT, Simon ,  BOVET, Lucien ,  SIERRO, Nicolas

    IPC分类号： C12N15/82 ,  C12N9/02 ,  A01H5/00

    CPC分类号： C12N15/8243 ,  C12N9/0073 ,  C12N15/8218 ,  C12Q1/6895 ,  C12Q2600/13 ,  C12Q2600/156 ,  C12Y114/13

    摘要： The present invention relates to a mutant, non-naturally occurring or transgenic tobacco plant cell comprising: (i) a polynucleotide comprising, consisting or consisting essentially of a sequence encoding a functional nicotine N-demethylase and having at least 95% sequence identity to SEQ ID NO:2; (ii) a polypeptide encoded by the polynucleotide set forth in (i); (iii) a polypeptide comprising, consisting or consisting essentially of a sequence encoding a nicotine N-demethylase and having at least 95% sequence identity to SEQ ID NO:3; or (iv) a construct, vector or expression vector comprising the isolated polynucleotide set forth in (i), and wherein the expression or activity of said nicotine demethylase is reduced as compared to a control tobacco plant cell in which the expression or activity of said nicotine demethylase has not been reduced.

    摘要翻译： 本发明涉及一种突变体，非天然存在或转基因烟草植物细胞，其包含：（i）多核苷酸，其包含，组成或者基本上由编码功能性尼古丁N-脱甲基酶的序列组成，并且与SEQ ID不得少于95％的序列同一性 编号：2; （ii）由（i）所述的多核苷酸编码的多肽; （iii）一种多肽，其包含由编码尼古丁N-脱甲基酶并与SEQ ID NO：3具有至少95％序列同一性的序列组成或由其组成; 或者（iv）包含（i）所述的分离的多核苷酸的构建体，载体或表达载体，并且其中所述尼古丁脱甲基酶的表达或活性与所述烟碱脱甲基酶的表达或活性相比降低， 尼古丁脱甲基酶尚未减少。

    EXTRACELLULAR DITERPENE PRODUCTION
    4.
    发明公开
    EXTRACELLULAR DITERPENE PRODUCTION 审中-公开
    细胞二萜生产

    公开(公告)号：EP3004365A1

    公开(公告)日：2016-04-13

    申请号：EP14727820.4

    申请日：2014-06-02

    申请人： DSM IP Assets B.V.

    发明人： BOER, Viktor Marius ,  BROERS, Nicolette, Jasmijn ,  LAWRENCE, Adam, G.

    IPC分类号： C12P15/00 ,  C12P19/56 ,  A23L27/30 ,  A23L2/60 ,  C12N9/90 ,  C12N9/88 ,  C12N9/02 ,  C12N9/10 ,  C12N15/52

    CPC分类号： C12P19/56 ,  A23K20/105 ,  A23L2/60 ,  A23L27/36 ,  A23V2002/00 ,  C07H15/256 ,  C12N9/0042 ,  C12N9/0073 ,  C12N9/0083 ,  C12N9/1051 ,  C12N9/88 ,  C12N9/90 ,  C12N15/52 ,  C12P15/00 ,  C12Y114/13 ,  C12Y114/13078 ,  C12Y402/03019 ,  C12Y505/01013

    摘要： The present invention relates to a method for the production of a diterpene or a glycosylated diterpene, which method comprises: a. fermenting a recombinant microorganism in a suitable fermentation medium, wherein the microorganism comprises one or more nucleotide sequence(s) encoding: a polypeptide having ent-copalyl pyrophosphate synthase activity; a polypeptide having ent-Kaurene synthase activity; a polypeptide having ent-Kaurene oxidase activity; and a polypeptide having kaurenoic acid 13-hydroxylase activity and whereby expression of the nucleotide sequence(s) confer(s) on the microorganism the ability to produce at least steviol, whereby a diterpene or glycosylated diterpene is produced extracellularly in the fermentation medium; and b. recovering the diterpene or glycosylated diterpene from the fermentation medium.

    摘要翻译： 本发明涉及生产二萜或糖基化双萜的方法，该方法包括：a。 在合适的发酵培养基中发酵重组微生物，其中所述微生物包含一种或多种编码以下核苷酸序列的核苷酸序列：具有对焦copalyl焦磷酸合酶活性的多肽; 具有ent-Kaurene合酶活性的多肽; 具有ent-Kaurene氧化酶活性的多肽; 和具有kaurenoic acid 13-羟化酶活性的多肽，并且其中所述核苷酸序列的表达赋予微生物至少产生甜菊醇的能力，由此在发酵培养基中在细胞外产生二萜或糖基化双萜; 和b。 从发酵培养基中回收二萜或糖基化的二萜。