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公开(公告)号：EP1893638A2
公开(公告)日：2008-03-05
申请号：EP06772278.5
申请日：2006-06-06
发明人： ABAD, Andre R. , CHUN, Gary C. , FLANNAGAN, Ronald D. , HERRMANN, Rafael , LU, Albert L. , MCCUTCHEN, Billy F. , RICE, Janet A. , SCHEPERS, Eric J.
IPC分类号： C07K14/325 , C12N9/64 , A01N63/02 , C12N15/82
CPC分类号： C12N15/8286 , A01N63/02 , C07K14/325 , C12N9/6472 , Y02A40/162
摘要： Compositions and methods for protecting a plant from an insect pest are provided. Nucleic acid molecules encoding insect protoxins or insect toxins modified to comprise at least one proteolytic activation site that is sensitive to an insect gut protease are provided. Cleavage of a modified insect protoxin at the proteolytic activation site by an insect gut protease produces an active insect toxin in the gut of the insect pest. Cleavage of a modified insect toxin of the invention at a proteolytic activation site results in the production of an active insect toxin in the insect gut that displays improved pesticidal activity relative to the insect toxin that lacks the proteolytic activation site. Methods of using the modified insect protoxin and modified insect toxin nucleic acid sequences and the polypeptides they encode to protect a plant from an insect pest are provided. Particular embodiments of the invention further provide modified insect protoxin and modified insect toxin compositions and formulations, expression cassettes, and transformed plants, plant cells, and seeds. Insect gut proteases and the nucleic acid molecules that encode them are also disclosed herein.
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公开(公告)号：EP3207143A1
公开(公告)日：2017-08-23
申请号：EP15790338.6
申请日：2015-10-14
发明人： BARRY, Jennifer K. , CLARK, Deborah W. , ENGLISH, James J. , ONG, Azalea , SCHEPERS, Eric J. , QI, Julie , RICE, Janet A.
IPC分类号： C12N15/82 , C07K14/415 , A01N65/00
CPC分类号： C07K14/415 , A01N37/46 , A01N47/44 , A01N65/00 , A01N65/04 , C07K2319/00 , C12N15/8286 , Y02A40/162
摘要： Compositions and methods for controlling pests are provided. The methods involve transforming organisms with a nucleic acid sequence encoding an insecticidal protein. In particular, the nucleic acid sequences are useful for preparing plants and microorganisms that possess insecticidal activity. Thus, transformed bacteria, plants, plant cells, plant tissues and seeds are provided. Compositions are insecticidal nucleic acids and proteins of bacterial species. The sequences find use in the construction of expression vectors for subsequent transformation into organisms of interest including plants, as probes for the isolation of other homologous (or partially homologous) genes. The pesticidal proteins find use in controlling, inhibiting growth or killing Lepidopteran, Coleopteran, Dipteran, fungal, Hemipteran and nematode pest populations and for producing compositions with insecticidal activity.
摘要翻译： 提供了用于防治害虫的组合物和方法。 该方法涉及用编码杀虫蛋白质的核酸序列转化生物体。 特别地，核酸序列可用于制备具有杀虫活性的植物和微生物。 因此，提供了转化的细菌，植物，植物细胞，植物组织和种子。 组合物是杀虫核酸和细菌物种的蛋白质。 该序列可用于构建表达载体，用于随后转化成感兴趣的生物体，包括植物，作为分离其他同源（或部分同源）基因的探针。 杀虫蛋白质可用于控制，抑制鳞翅目，鞘翅目，双翅目，真菌，半翅目和线虫害虫种群的生长或杀死以及用于产生具有杀虫活性的组合物。
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3.发明授权
公开(公告)号：EP2235182B1
公开(公告)日：2013-07-17
申请号：EP09702253.7
申请日：2009-01-15
发明人： HERRMANN, Rafael , LASSNER, Michael , LU, Albert L. , NELSON, Mark , PRESNAIL, James K. , RICE, Janet A.
IPC分类号： C12N15/82
CPC分类号： C12N15/8286 , A01N65/20 , A01N65/44 , C12N15/113 , C12N2310/11 , C12N2310/14 , C12N2310/531 , C12N2320/32 , Y02A40/162
摘要： Methods and compositions are provided which employ a silencing element that, when ingested by a pest, such as a pest from the Lepidoptera order, they are capable of decreasing the expression of a target sequence in the pest. In specific embodiments, the decrease in expression of the target sequence controls the pest and thereby the methods and compositions are capable of limiting damage to a plant. The present invention provides target polynucleotides encoding polypeptides from specific protein families and various target polynucleotides set forth in SEQ ID NOS: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, or 50 or active variants and fragments thereof, wherein a decrease in expression of one or more the sequences in the target pest controls the pest (i.e., has insecticidal activity). Further provided are silencing elements which when ingested by the pest decrease the level of the target polypeptide and thereby control the pest. In specific embodiment, the pest is Spodoptera frugiperda. Plants, plant part, bacteria and other host cells comprising the silencing elements or an active variant or fragment thereof of the invention are also provided.
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4.发明公开
公开(公告)号：EP2235182A2
公开(公告)日：2010-10-06
申请号：EP09702253.7
申请日：2009-01-15
发明人： HERRMANN, Rafael , LASSNER, Michael , LU, Albert L. , NELSON, Mark , PRESNAIL, James K. , RICE, Janet A.
IPC分类号： C12N15/82
CPC分类号： C12N15/8286 , A01N65/20 , A01N65/44 , C12N15/113 , C12N2310/11 , C12N2310/14 , C12N2310/531 , C12N2320/32 , Y02A40/162
摘要： Methods and compositions are provided which employ a silencing element that, when ingested by a pest, such as a pest from the Lepidoptera order, they are capable of decreasing the expression of a target sequence in the pest. In specific embodiments, the decrease in expression of the target sequence controls the pest and thereby the methods and compositions are capable of limiting damage to a plant. The present invention provides target polynucleotides encoding polypeptides from specific protein families and various target polynucleotides set forth in SEQ ID NOS: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, or 50 or active variants and fragments thereof, wherein a decrease in expression of one or more the sequences in the target pest controls the pest (i.e., has insecticidal activity). Further provided are silencing elements which when ingested by the pest decrease the level of the target polypeptide and thereby control the pest. In specific embodiment, the pest is Spodoptera frugiperda. Plants, plant part, bacteria and other host cells comprising the silencing elements or an active variant or fragment thereof of the invention are also provided.
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公开(公告)号：EP1893638B1
公开(公告)日：2011-08-10
申请号：EP06772278.5
申请日：2006-06-06
发明人： ABAD, Andre R. , CHUN, Gary C. , FLANNAGAN, Ronald D. , HERRMANN, Rafael , LU, Albert L. , MCCUTCHEN, Billy F. , RICE, Janet A. , SCHEPERS, Eric J.
IPC分类号： C07K14/325 , C12N9/64 , A01N63/02 , C12N15/82
CPC分类号： C12N15/8286 , A01N63/02 , C07K14/325 , C12N9/6472 , Y02A40/162
摘要： Compositions and methods for protecting a plant from an insect pest are provided. Nucleic acid molecules encoding insect protoxins or insect toxins modified to comprise at least one proteolytic activation site that is sensitive to an insect gut protease are provided. Cleavage of a modified insect protoxin at the proteolytic activation site by an insect gut protease produces an active insect toxin in the gut of the insect pest. Cleavage of a modified insect toxin of the invention at a proteolytic activation site results in the production of an active insect toxin in the insect gut that displays improved pesticidal activity relative to the insect toxin that lacks the proteolytic activation site. Methods of using the modified insect protoxin and modified insect toxin nucleic acid sequences and the polypeptides they encode to protect a plant from an insect pest are provided. Particular embodiments of the invention further provide modified insect protoxin and modified insect toxin compositions and formulations, expression cassettes, and transformed plants, plant cells, and seeds. Insect gut proteases and the nucleic acid molecules that encode them are also disclosed herein.
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