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1.发明授权
公开(公告)号：EP2235182B1
公开(公告)日：2013-07-17
申请号：EP09702253.7
申请日：2009-01-15
发明人： HERRMANN, Rafael , LASSNER, Michael , LU, Albert L. , NELSON, Mark , PRESNAIL, James K. , RICE, Janet A.
IPC分类号： C12N15/82
CPC分类号： C12N15/8286 , A01N65/20 , A01N65/44 , C12N15/113 , C12N2310/11 , C12N2310/14 , C12N2310/531 , C12N2320/32 , Y02A40/162
摘要： Methods and compositions are provided which employ a silencing element that, when ingested by a pest, such as a pest from the Lepidoptera order, they are capable of decreasing the expression of a target sequence in the pest. In specific embodiments, the decrease in expression of the target sequence controls the pest and thereby the methods and compositions are capable of limiting damage to a plant. The present invention provides target polynucleotides encoding polypeptides from specific protein families and various target polynucleotides set forth in SEQ ID NOS: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, or 50 or active variants and fragments thereof, wherein a decrease in expression of one or more the sequences in the target pest controls the pest (i.e., has insecticidal activity). Further provided are silencing elements which when ingested by the pest decrease the level of the target polypeptide and thereby control the pest. In specific embodiment, the pest is Spodoptera frugiperda. Plants, plant part, bacteria and other host cells comprising the silencing elements or an active variant or fragment thereof of the invention are also provided.
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公开(公告)号：EP3341483B1
公开(公告)日：2019-12-18
申请号：EP16763644.8
申请日：2016-08-26
发明人： ANAND, Ajith , BASS, Steven Henry , BERTAIN, Sean M. , CHO, Hyeon-Je , KINNEY, Anthony J , KLEIN, Theodore M. , LASSNER, Michael , MCBRIDE, Kevin E , MOY, York , ROSEN, Barabara Ann Marie , WEI, Jun-Zhi
IPC分类号： C12N15/82 , C07K14/195
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3.发明公开
公开(公告)号：EP2281049A2
公开(公告)日：2011-02-09
申请号：EP09701991.3
申请日：2009-01-15
发明人： HERRMANN, Rafael , LASSNER, Michael , LU, Albert, L. , NELSON, Mark , PRESNAIL, James, K. , RICE, Janet, A.
IPC分类号： C12N15/82
CPC分类号： C12N15/8286 , C07K14/43563 , Y02A40/162
摘要： Methods and compositions are provided which employ a silencing element that, when ingested by a pest, such as a pest from the Aphididae family, they are capable of decreasing the expression of a target sequence in the pest. In specific embodiments, the decrease in expression of the target sequence controls the pest and thereby the methods and compositions are capable of limiting damage to a plant. The present invention provides target polynucleotides for specific protein classes and also target polynucleotides as set forth in SEQ ID NOS:1, 2, 3, 4, 5, 6, 7, 8, 9, 10 or active variants or fragments thereof, wherein a decrease in expression of one or more the sequences in the target pest controls the pest (i.e., has insecticidal activity). Further provided are silencing elements which when ingested by the pest decrease the level of the target polypeptide and thereby control the pest. In specific embodiment, the pest is Aphis glycines. Plants, plant part, bacteria and other host cells comprising the silencing elements or an active variant or fragment thereof of the invention are also provided.
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公开(公告)号：EP3341483A1
公开(公告)日：2018-07-04
申请号：EP16763644.8
申请日：2016-08-26
发明人： ANAND, Ajith , BASS, Steven Henry , BERTAIN, Sean M. , CHO, Hyeon-Je , KINNEY, Anthony J , KLEIN, Theodore M. , LASSNER, Michael , MCBRIDE, Kevin E , MOY, York , ROSEN, Barabara Ann Marie , WEI, Jun-Zhi
IPC分类号： C12N15/82 , C07K14/195
摘要： Methods and compositions for Ochrobactrum-mediated transformation of plants are provided. Methods include but are not limited to using an Ochrobactrum strain to transfer a polynucleotide of interest to a plant cell. These include VirD2-dependent methods. Compositions include an Ochrobactrum strain, transfer DNAs, constructs and/or plasmids. These include Ochrobactrum strains having a plasmid comprising one or more virulence gene(s), border region, and/or origin of replication. Plant cells, tissues, plants, and seeds comprising a polynucleotide of interest produced by the methods are also provided.
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5.发明公开COMPOSITIONS AND METHODS FOR PRODUCING PLANTS RESISTANT TO GLYPHOSATE HERBICIDE 审中-公开用于制造抗耐药性植物的组合物和方法草甘膦除草剂
公开(公告)号：EP3166391A1
公开(公告)日：2017-05-17
申请号：EP15739713.4
申请日：2015-07-01
发明人： DJUKANOVIC, Vesna , JONES, Spencer Charles , LIU, Zhan-Bin , LASSNER, Michael , LYZNIK, Leszek, Aleksander
IPC分类号： A01H1/02 , C12N15/113 , C12N15/82
CPC分类号： C12N15/8275 , A01H1/02 , A01H4/008 , C12N15/8213 , C12N15/8241 , C12N2310/20 , C12Y301/00
摘要： The present disclosure includes the production of a mutant plant resistant to an herbicide of the phosphonomethylglycine family, e.g. glyphosate. Compositions and methods are provided for editing a nucleotide sequence of interest in a cell employing a guide polynucleotide/Cas endonuclease system, wherein the Cas endonuclease is guided by a guide polynucleotide to recognize and optionally introduce a double strand break at a specific target site into the genome of a cell. The nucleotide sequence of interest to be edited can be located within or outside the target site that is recognized by a Cas endonuclease. More specifically, compositions and methods are provided for editing an enolpyruvylshikimate-3-phosphate synthase (EPSPS) nucleotide sequence in a cell. The methods and compositions employ a guide polynucleotide/Cas endonuclease system to provide for an effective system for editing EPSPS nucleotide sequences of within the genome of a cell. Also provided are compositions and methods for the production of glyphosate tolerant plant cells, plants explants, seeds and grain.
摘要翻译： 本公开包括在phosphonomethylglycines家族的除草剂的生产突变植物抗性，例如 草甘膦。 提供了用于在细胞中编辑感兴趣的核苷酸序列采用导向的多核苷酸/ CAS核酸内切酶系统，worin中科院内切核酸酶通过导向的多核苷酸导入到识别和任选的特定靶位点到引入双海滩断组合物和方法 细胞的基因组中。 要被编辑的感兴趣的核苷酸序列可以位于内或在靶位点外也通过将CAS核酸内切酶识别。 更具体地，组合物和方法提供了用于在细胞烯醇丙酮莽草酸-3-磷酸合酶（EPSPS）的核苷酸序列的编辑。 所述方法和组合物使用的多核苷酸导/ CAS核酸内切酶在系统有效的系统，以提供用于编辑EPSPS细胞内的基因组中的核苷酸序列。 因此，提供了组合物和方法用于生产草甘膦耐性植物细胞，植物外植体，种子和谷物。
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6.发明公开METHODS AND COMPOSITIONS FOR GENERATING COMPLEX TRAIT LOCI 审中-公开VERFAHREN UND ZUSAMMENSETZUNGEN ZUR ERZEUGUNG KOMPLEXER TRAIT LOCI
公开(公告)号：EP2807257A1
公开(公告)日：2014-12-03
申请号：EP13702566.4
申请日：2013-01-24
CPC分类号： A01H5/10 , A01H1/02 , C12N9/22 , C12N15/8213
摘要： Compositions and methods are provided for stacking multiple independent transgenic loci into the genome of a plant. Compositions include plants, seeds or plant cells comprising at least one transgenic target site and at least one genomic locus of interest integrated at different genomic sites within a genomic window. Plant breeding techniques can be employed such that the transgenic target site and the genomic locus of interest can be bred together. In this way, multiple independent transgene integrations can be generated within a genomic window to create a complex trait locus. The complex trait locus is designed such that the transgenic target sites and/or genomic loci of interest can segregate independently of each other, thus providing the benefit of altering a complex trait locus by breeding-in and breeding-away specific elements. Various methods can also be employed to modify the target sites such that they contain a variety of polynucleotides of interest.
摘要翻译： 提供了将多个独立的转基因基因座堆叠到植物基因组中的组合物和方法。 组合物包括植物，种子或植物细胞，其包含至少一个转基因靶位点和在基因组窗口内的不同基因组位点处整合的至少一个感兴趣的基因组。 可以使用植物育种技术，使得转基因靶位点和感兴趣的基因组座位可以一起繁殖。 以这种方式，可以在基因组窗口内产生多个独立的转基因整合以产生复杂的性状基因座。 复杂特征位点被设计成使得目的基因座和/或基因座位点彼此独立地分离，从而提供通过繁殖和遗传特异性元件改变复杂性状基因座的益处。 还可以使用各种方法来修饰靶位点，使得它们含有多种感兴趣的多核苷酸。
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7.发明公开METHODS FOR PRODUCING GENETIC MODIFICATIONS IN A PLANT GENOME WITHOUT INCORPORATING A SELECTABLE TRANSGENE MARKER, AND COMPOSITIONS THEREOF 审中-公开用于生产基因突变的植物基因组无可选择的转基因标志物及其组合物
公开(公告)号：EP3036333A1
公开(公告)日：2016-06-29
申请号：EP14761478.8
申请日：2014-08-20
发明人： CIGAN, Andrew Mark , FALCO, Saverio, Carl , LASSNER, Michael , LIU, Zhan-Bin , SVITASHEV, Sergei
IPC分类号： C12N15/82
CPC分类号： C12N15/8216 , C12N15/113 , C12N15/81 , C12N15/8213 , C12N15/8241 , C12N15/8245 , C12N15/8247 , C12N15/8261 , C12N15/8273 , C12N15/8274 , C12N15/8279 , C12N15/8286 , C12N2310/10 , C12N2310/20 , C12N2310/315 , C12N2310/3231 , C12N2310/3341 , C12Q1/6895 , C12Q2600/13 , C12Q2600/156 , C12N2310/321 , C12N2310/3521 , C12N2310/322 , C12N2310/3533
摘要： Compositions and methods are provided for genome modification of a target sequence in the genome of a plant or plant cell. The methods and compositions employ a guide RNA/Cas endonuclease system to provide an effective system for modifying or altering target sites within the genome of a plant, plant cell or seed. Also provided are compositions and methods employing a guide polynucleotide/Cas endonuclease system for genome modification of a nucleotide sequence in the genome of a cell or organism, for gene editing, and/or for inserting or deleting a polynucleotide of interest into or from the genome of a cell or organism. Once a genomic target site is identified, a variety of methods can be employed to further modify the target sites such that they contain a variety of polynucleotides of interest. Breeding methods and methods for selecting plants utilizing a two component RNA guide and Cas endonuclease system are also disclosed. Compositions and methods are also provided for editing a nucleotide sequence in the genome of a cell.
摘要翻译： 提供了用于在植物或植物细胞的基因组中的靶序列的基因组修饰组合物和方法。 方法和组合物使用的引导RNA / CAS核酸内切酶系统提供用于规划细胞或种子的基因组内的修改或改变靶位点上有效的系统的计划。 如此提供的组合物和方法采用用于在细胞或生物体的基因组的核苷酸序列的基因组修饰的导向多核苷酸/ CAS核酸内切酶系统中，用于基因编辑，和/或用于插入或删除的目标多核苷酸中或从基因组 细胞或生物体的。 一旦基因组靶部位被识别，多种方法可以采用祖weiterer修改审查的靶位点thatthey含有多种兴趣多核苷酸。 因此育种方法和用于选择植物利用的双组分RNA引导和CAS核酸内切酶系统的方法是游离缺失盘。 因此，提供一种用于在细胞的基因组编辑的核苷酸序列的组合物和方法。
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公开(公告)号：EP2364322A1
公开(公告)日：2011-09-14
申请号：EP09759826.2
申请日：2009-10-22
IPC分类号： C07K14/195
CPC分类号： C07K14/245 , C07K14/195 , C12N15/635 , C12N15/8217 , C12N15/8238 , C12N15/8278
摘要： Compositions and methods relating to the use of sulfonylurea-responsive repressors are provided. Compositions include polypeptides that specifically bind to an operator, wherein the specific binding is regulated by a sulfonylurea compound. Compositions also include polynucleotides encoding the polypeptides as well as constructs, vectors, prokaryotic and eukaryotic cells, and eukaryotic organisms including plants and seeds comprising the polynucleotide, and/or produced by the methods. Also provided are methods to provide a sulfonylurea-responsive repressor to a cell or organism, and to regulate expression of a polynucleotide of interest in a cell or organism, including a plant or plant cell.
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9.发明公开
公开(公告)号：EP2235182A2
公开(公告)日：2010-10-06
申请号：EP09702253.7
申请日：2009-01-15
发明人： HERRMANN, Rafael , LASSNER, Michael , LU, Albert L. , NELSON, Mark , PRESNAIL, James K. , RICE, Janet A.
IPC分类号： C12N15/82
CPC分类号： C12N15/8286 , A01N65/20 , A01N65/44 , C12N15/113 , C12N2310/11 , C12N2310/14 , C12N2310/531 , C12N2320/32 , Y02A40/162
摘要： Methods and compositions are provided which employ a silencing element that, when ingested by a pest, such as a pest from the Lepidoptera order, they are capable of decreasing the expression of a target sequence in the pest. In specific embodiments, the decrease in expression of the target sequence controls the pest and thereby the methods and compositions are capable of limiting damage to a plant. The present invention provides target polynucleotides encoding polypeptides from specific protein families and various target polynucleotides set forth in SEQ ID NOS: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, or 50 or active variants and fragments thereof, wherein a decrease in expression of one or more the sequences in the target pest controls the pest (i.e., has insecticidal activity). Further provided are silencing elements which when ingested by the pest decrease the level of the target polypeptide and thereby control the pest. In specific embodiment, the pest is Spodoptera frugiperda. Plants, plant part, bacteria and other host cells comprising the silencing elements or an active variant or fragment thereof of the invention are also provided.
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公开(公告)号：EP1781795B1
公开(公告)日：2011-01-19
申请号：EP05857490.6
申请日：2005-06-30
IPC分类号： C12N15/82
CPC分类号： C12N15/8282
摘要： Methods for protecting a plant from a plant pathogenic fungus are provided. A method for enhancing fungal pathogen resistance in a plant using the nucleotide sequences disclosed herein is further provided. The method comprises introducing into a plant an expression cassette comprising a promoter operably linked to a nucleotide sequence that encodes an antifungal polypeptide of the invention. Transformed plants, plant cells, seeds, and microorganisms comprising a nucleotide sequence that encodes an antifungal polypeptide of the embodiments, or variant or fragment thereof, are also disclosed.
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