公开(公告)号：EP1763536B1

公开(公告)日：2010-09-08

申请号：EP05788923.0

申请日：2005-07-01

申请人： Pioneer-Hi-Bred International, Inc. ,  E. I. du Pont de Nemours and Company ,  The Regents of The University of California

发明人： ALTIER, Daniel, J. ,  DAHLBACKA, Glen ,  HERRMANN, Rafael ,  HUNTER-CEVERA, Jennie ,  McCUTCHEN, Billy, Fred ,  PRESNAIL, James, K. ,  SCHEPERS, Eric, J. ,  RICE, Janet, A. ,  SIMMONS, Carl, R. ,  TOROK, Tamas ,  YALPANI, Nasser ,  ELLESKAYA, I., A.

IPC分类号： C07K14/385 ,  C07K14/37

CPC分类号： C07K14/37 ,  A01N63/02 ,  A01N63/04 ,  A61K38/00 ,  C07K14/385 ,  C12N15/8282

摘要： Compositions and methods for protecting a plant from a pathogen, particularly a fungal pathogen, are provided. Compositions include novel amino acid sequences, and variants and fragments thereof, for antipathogenic polypeptides that were isolated from microbial fermentation broths. Nucleic acid molecules comprising nucleotide sequences that encode the antipathogenic polypeptides of the invention are also provided. A method for inducing pathogen resistance in a plant using the nucleotide sequences disclosed herein is further provided. The method comprises introducing into a plant an expression cassette comprising a promoter operably linked to a nucleotide sequence that encodes an antipathogenic polypeptide of the invention. Compositions comprising an antipathogenic polypeptide or a transformed microorganism comprising a nucleic acid of the invention in combination with a carrier and methods of using these compositions to protect a plant from a pathogen are further provided. Transformed plants, plant cells, seeds, and microorganisms comprising a nucleotide sequence that encodes an antipathogenic polypeptide of the invention, or variant or fragment thereof, are also disclosed.

公开(公告)号：EP2281049A2

公开(公告)日：2011-02-09

申请号：EP09701991.3

申请日：2009-01-15

申请人： Pioneer Hi-Bred International Inc. ,  E. I. du Pont de Nemours and Company

发明人： HERRMANN, Rafael ,  LASSNER, Michael ,  LU, Albert, L. ,  NELSON, Mark ,  PRESNAIL, James, K. ,  RICE, Janet, A.

IPC分类号： C12N15/82

CPC分类号： C12N15/8286 ,  C07K14/43563 ,  Y02A40/162

摘要： Methods and compositions are provided which employ a silencing element that, when ingested by a pest, such as a pest from the Aphididae family, they are capable of decreasing the expression of a target sequence in the pest. In specific embodiments, the decrease in expression of the target sequence controls the pest and thereby the methods and compositions are capable of limiting damage to a plant. The present invention provides target polynucleotides for specific protein classes and also target polynucleotides as set forth in SEQ ID NOS:1, 2, 3, 4, 5, 6, 7, 8, 9, 10 or active variants or fragments thereof, wherein a decrease in expression of one or more the sequences in the target pest controls the pest (i.e., has insecticidal activity). Further provided are silencing elements which when ingested by the pest decrease the level of the target polypeptide and thereby control the pest. In specific embodiment, the pest is Aphis glycines. Plants, plant part, bacteria and other host cells comprising the silencing elements or an active variant or fragment thereof of the invention are also provided.

公开(公告)号：EP1763536A1

公开(公告)日：2007-03-21

申请号：EP05788923.0

申请日：2005-07-01

申请人： Pioneer-Hi-Bred International, Inc. ,  E.I. du Pont de Nemours and Company ,  The Regents of the University of California

发明人： ALTIER, Daniel, J., Altier ,  DAHLBACKA, Glen ,  HERRMANN, Rafael ,  HUNTER-CEVERA, Jennie ,  McCUTCHEN, Billy, Fred ,  PRESNAIL, James, K. ,  SCHEPERS, Eric, J. ,  RICE, Janet, A. ,  SIMMONS, Carl, R. ,  TOROK, Tamas ,  YALPANI, Nasser ,  ELLESKAYA, I., A.

IPC分类号： C07K14/385 ,  C07K14/37

CPC分类号： C07K14/37 ,  A01N63/02 ,  A01N63/04 ,  A61K38/00 ,  C07K14/385 ,  C12N15/8282

摘要： Compositions and methods for protecting a plant from a pathogen, particularly a fungal pathogen, are provided. Compositions include novel amino acid sequences, and variants and fragments thereof, for antipathogenic polypeptides that were isolated from microbial fermentation broths. Nucleic acid molecules comprising nucleotide sequences that encode the antipathogenic polypeptides of the invention are also provided. A method for inducing pathogen resistance in a plant using the nucleotide sequences disclosed herein is further provided. The method comprises introducing into a plant an expression cassette comprising a promoter operably linked to a nucleotide sequence that encodes an antipathogenic polypeptide of the invention. Compositions comprising an antipathogenic polypeptide or a transformed microorganism comprising a nucleic acid of the invention in combination with a carrier and methods of using these compositions to protect a plant from a pathogen are further provided. Transformed plants, plant cells, seeds, and microorganisms comprising a nucleotide sequence that encodes an antipathogenic polypeptide of the invention, or variant or fragment thereof, are also disclosed.

公开(公告)号：EP1709068A2

公开(公告)日：2006-10-11

申请号：EP04814870.4

申请日：2004-12-20

申请人： PIONEER HI-BRED INTERNATIONAL, INC. ,  E.I. du Pont de Nemours and Company

发明人： ABAD, André ,  FLANNAGAN, Ronald, D. ,  HERRMANN, Rafael ,  LU, Albert ,  MCCUTCHEN, Billy, F. ,  PRESNAIL, James, K. ,  RICE, Janet, A. ,  WONG, James, F. ,  YU, Cao-Guo

IPC分类号： C07K14/325 ,  C12N9/64 ,  C12N15/82 ,  A01H5/02

CPC分类号： C12N15/8286 ,  C12N9/641 ,  Y02A40/162

摘要： Compositions and methods for protecting a plant from an insect pest are provided. In particular, nucleic acid sequences encoding insect protoxins modified to comprise at least one proteolytic activation site that is sensitive to a plant protease or an insect gut protease are provided. Cleavage of the modified protoxin at the proteolytic activation site by a protease produces an active insect toxin. Methods of using the modified insect protoxin nucleic acid sequences and the polypeptides they encode to protect a plant from an insect pest are provided. Particular embodiments of the invention further provide modified insect protoxin compositions and formulations, expression cassettes, and transformed plants, plant cells, and seeds.

公开(公告)号：EP1699816A2

公开(公告)日：2006-09-13

申请号：EP04813794.7

申请日：2004-12-09

申请人： PIONEER HI-BRED INTERNATIONAL, INC. ,  E.I. du Pont de Nemours and Company

发明人： ABAD, André ,  DONG, Hua ,  HERRMANN, Rafael ,  LU, Albert ,  McCUTCHEN, Billy, F. ,  RICE, Janet, A. ,  SCHEPERS, Eric, J. ,  WONG, James, F.

IPC分类号： C07K14/325 ,  C12N15/82

CPC分类号： C07K14/325 ,  C12N15/8286 ,  Y02A40/162

摘要： Compositions and methods for protecting a plant from an insect pest are provided. The invention provides mutagenized nucleic acids that have been engineered to encode pesticidal polypeptides having increased resistance to proteolytic degradation by a plant protease. In particular, nucleic acid sequences encoding pesticidal polypeptides modified to comprise a proteolytic protection site that confers resistance to degradation or proteolytic inactivation by a plant protease are provided. Particular embodiments of the invention provide expression cassettes and transformed plants, plant cells, and seeds. These compositions find use in methods for protecting a plant from a pest.