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公开(公告)号：EP2235210B1
公开(公告)日：2015-03-25
申请号：EP08865992.5
申请日：2008-12-19
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6874 , C12Q2537/143 , C12Q2563/149
摘要： The present invention relates to systems and methods for sequencing nucleic acids, including sequencing nucleic acids in fluidic droplets. In one set of embodiments, the method employs sequencing by hybridization using droplets such as microfluidic droplets. In some embodiments, droplets are formed which include a target nucleic acid, a nucleic acid probe, and at least one identification element, such as a fluorescent particle. The nucleic acid probes that hybridize to the target nucleic acid are determined, in some instances, by determining the at least one identification element. The nucleic acid probes that hybridize to the target nucleic acid may be used to determine the sequence of the target nucleic acid. In certain instances, the microfluidic droplets are provided with reagents that modify the nucleic acid probe. In some cases, a droplet, such as those described above, is deformed such that the components of the droplets individually pass a target area.
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公开(公告)号：EP4019977A1
公开(公告)日：2022-06-29
申请号：EP21208171.5
申请日：2010-06-25
发明人： WEITZ, David A. , ABATE, Adam R. , HUNG, Tony , MARY, Pascaline
IPC分类号： G01N35/10 , B01J19/00 , B01F25/314 , B01F33/3031
摘要： Systems (200) and methods for the control of fluids and, in some cases, to systems and methods for flowing a fluid into and/or out of other fluids. As examples, fluid may be injected into a droplet (220) contained within a fluidic channel (230), or a fluid may be injected into a fluidic channel to create a droplet (220). In some embodiments, electrodes (250) may be used to apply an electric field to one or more fluidic channels (230,240), e.g., proximate an intersection of at least two fluidic channels (230, 240). For instance, a first fluid may be urged into and/or out of a second fluid, facilitated by the electric field. The electric field, in some cases, may disrupt an interface between a first fluid and at least one other fluid. Properties such as the volume, flow rate, etc. of a first fluid being urged into and/or out of a second fluid can be controlled by controlling various properties of the fluid and/or a fluidic droplet, for example curvature of the fluidic droplet, and/or controlling the applied electric field.
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公开(公告)号：EP2235210A1
公开(公告)日：2010-10-06
申请号：EP08865992.5
申请日：2008-12-19
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6874 , C12Q2537/143 , C12Q2563/149
摘要： The present invention relates to systems and methods for sequencing nucleic acids, including sequencing nucleic acids in fluidic droplets. In one set of embodiments, the method employs sequencing by hybridization using droplets such as microfluidic droplets. In some embodiments, droplets are formed which include a target nucleic acid, a nucleic acid probe, and at least one identification element, such as a fluorescent particle. The nucleic acid probes that hybridize to the target nucleic acid are determined, in some instances, by determining the at least one identification element. The nucleic acid probes that hybridize to the target nucleic acid may be used to determine the sequence of the target nucleic acid. In certain instances, the microfluidic droplets are provided with reagents that modify the nucleic acid probe. In some cases, a droplet, such as those described above, is deformed such that the components of the droplets individually pass a target area.
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公开(公告)号：EP2446278A2
公开(公告)日：2012-05-02
申请号：EP10792729.5
申请日：2010-06-25
发明人： WEITZ, David, A. , ABATE, Adam, R. , HUNG, Tony , MARY, Pascaline
CPC分类号： B01F5/0471 , B01F13/0071 , B01F13/0076 , B01J19/0093 , B01J2219/00837 , B01J2219/00853 , B01J2219/00862 , B01J2219/00889 , B01L3/50273 , B01L3/502784 , B01L2300/0867 , B01L2400/0415 , F16K99/0042 , F16K99/0051 , G01N27/44791 , G01N2035/1034 , Y10T137/0391 , Y10T137/206
摘要： The present invention generally relates to systems and methods for the control of fluids and, in some cases, to systems and methods for flowing a fluid into and/or out of other fluids. As examples, fluid may be injected into a droplet contained within a fluidic channel, or a fluid may be injected into a fluidic channel to create a droplet. In some embodiments, electrodes may be used to apply an electric field to one or more fluidic channels, e.g., proximate an intersection of at least two fluidic channels. For instance, a first fluid may be urged into and/or out of a second fluid, facilitated by the electric field. The electric field, in some cases, may disrupt an interface between a first fluid and at least one other fluid. Properties such as the volume, flow rate, etc. of a first fluid being urged into and/or out of a second fluid can be controlled by controlling various properties of the fluid and/or a fluidic droplet, for example curvature of the fluidic droplet, and/or controlling the applied electric field.
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