公开(公告)号：EP2040746A1

公开(公告)日：2009-04-01

申请号：EP07809720.1

申请日：2007-06-19

申请人： Progenics Pharmaceuticals, Inc. ,  Cornell Research Foundation, Inc.

发明人： OLSON, William C. ,  MADDON, Paul J. ,  FRANTI, Michael ,  IYER, Sai Prasad N. ,  KANG, Kenneth ,  MOORE, John P. ,  BEDDOWS, Simon

IPC分类号： A61K39/21 ,  A61K39/00 ,  A61K39/12 ,  C07K2/00 ,  C07K17/00 ,  C07K1/00

CPC分类号： C07K14/005 ,  A61K39/00 ,  C12N2740/15022 ,  C12N2740/16122

摘要： This invention provides processes of purifying and recovering trimers of a retroviral envelope (Env) protein from a preparation comprising trimers of the retroviral Env protein and high molecular weight aggregates thereof. Non-ionic detergent is used in the processes to obtain a purified trimer product, which is separated from aggregates. The process further comprises treating the preparation with a non-ionic detergent so as to thereby convert the high molecular weight aggregates into trimers, and purifying and recovering the trimers from the preparation so treated.

摘要翻译： 本发明提供了从包含逆转录病毒Env蛋白质三聚体和其高分子量聚集体的制剂中纯化和回收逆转录病毒包膜（Env）蛋白质的三聚体的方法。 在工艺中使用非离子型洗涤剂以获得纯化的三聚体产物，其从聚集体分离。 该方法还包括用非离子型去污剂处理制剂，从而将高分子量聚集体转化为三聚体，并从如此处理的制备物中纯化并回收三聚体。

公开(公告)号：EP2040747A2

公开(公告)日：2009-04-01

申请号：EP07809721.9

申请日：2007-06-19

申请人： Progenics Pharmaceuticals, Inc. ,  Cornell Research Foundation, Inc.

发明人： OLSON, William C. ,  MADDON, Paul J. ,  FRANTI, Michael ,  IYER, Sai Prasad N. ,  ANDJELIC, Sofija ,  MOORE, John P. ,  BEDDOWS, Simon

IPC分类号： A61K39/21 ,  A61K39/12

CPC分类号： C07K16/1063 ,  A61K39/00 ,  A61K2039/55566 ,  A61K2039/55577 ,  C07K14/005 ,  C07K2317/76 ,  C12N2740/16122

摘要： This invention provides a protein comprising (a) a first polypeptide which comprises consecutive amino acids, the sequence of which corresponds to the sequence of a modified gpl20 envelope polypeptide portion of a gpl40 envelope of (i) an HIV-I KNH1144 isolate, or a quasi-species thereof, or (ii) an HIV-I 5768.4 isolate, or a quasi-species thereof; and (b) a second polypeptide which comprises consecutive amino acids, the sequence of which corresponds to the sequence of a modified gp41 ectodomain polypeptide portion of the gpl40 envelope of (i) the HIV-I KNHl144 isolate or such quasi-species thereof, or (ii) the HIV-I 5768.4 isolate or such quasi-species thereof. This invention also provides nucleic acids encoding such proteins, vectors, host cells and compositions thereof. Also provided are trimeric complexes ('trimers') of these proteins and methods of using such trimers to combat HIV-I infection.

公开(公告)号：EP2073840A2

公开(公告)日：2009-07-01

申请号：EP07870794.0

申请日：2007-10-17

申请人： Progenics Pharmaceuticals, Inc. ,  Cornell Research Foundation, Inc.

发明人： DEY, Antu K. ,  MOORE, John P. ,  OLSON, William C. ,  IYER, Sai Prasad N. ,  KANG, Kenneth ,  FRANTI, Michael

IPC分类号： A61K39/21 ,  A61K39/42 ,  C07H21/04

CPC分类号： A61K39/21 ,  A61K39/00 ,  A61K39/12 ,  C07K14/005 ,  C12N2740/16122 ,  C12N2740/16134

摘要： This invention provides a modified gpl40 envelope polypeptide of an HIV-1 isolate comprising a gpl20 polypeptide portion comprising consecutive amino acids and a gp41 ectodomain polypeptide portion comprising consecutive amino acids, said gp41 ectodomain polypeptide portion being modified to comprise isoleucine (I) at an amino acid position equivalent to amino acid position 535; glutamine (Q) at an amino acid position equivalent to amino acid position 543; serine (S) at an amino acid position equivalent to amino acid position 553; lysine (K) at an amino acid position equivalent to amino acid position 567; and arginine (R) at an amino acid position equivalent to amino acid position 588, the amino acid positions being numbered by reference to the HIV-1 isolate KNH1144. This invention also provides nucleic acids encoding such a polypeptide, vectors, host cells, trimeric complexes and compositions thereof. Also provided are antibodies generated against the modified polypeptides and trimeric complexes, and methods of using the modified polypeptides, compositions and trimeric complexes.