公开(公告)号：EP4177261A1

公开(公告)日：2023-05-10

申请号：EP22182344.6

申请日：2014-03-13

申请人： Promega Corporation

发明人： Dixon, Andrew S. ,  Encell, Lance ,  Hall, Mary ,  Wood, Keith ,  Wood, Monika ,  Schwinn, Marie ,  Binkowski, Brock F. ,  Zegzouti, Hicham ,  Nath, Nidhi ,  Mondal, Subhanjan ,  Goueli, Said ,  Meisenheimer, Poncho ,  Kirkland, Thomas ,  Unch, James ,  Pulukkunat, Dileep K. ,  Robers, Matthew ,  Dart, Melanie ,  Machleidt, Thomas

IPC分类号： C07K14/435 ,  G01N33/50

摘要： Provided herein are compositions and methods for the assembly of a bioluminescent complex from two or more non-luminescent (e.g., substantially non-luminescent) peptide and/or polypeptide units. In particular, bioluminescent activity is conferred upon a non-luminescent polypeptide via structural complementation with another, complementary non-luminescent peptide.

公开(公告)号：EP4169935A1

公开(公告)日：2023-04-26

申请号：EP22196592.4

申请日：2014-03-13

申请人： Promega Corporation

发明人： Dixon, Andrew S. ,  Encell, Lance P. ,  Hall, Mary P. ,  Wood, Keith V. ,  Wood, Monika G. ,  Schwinn, Marie ,  Binkowski, Brock ,  Zegzouti, Hicham ,  Nath, Nidhi ,  Mondal, Subhanjan ,  Goueli, Said ,  Meisenheimer, Poncho ,  Kirkland, Thomas ,  Unch, James ,  Pulukkunat, Dileep K. ,  Robers, Matthew B. ,  Dart, Melanie ,  Machleidt, Thomas

IPC分类号： C07K14/435 ,  G01N33/50

摘要： Provided herein are compositions and methods for the assembly of a bioluminescent complex from two or more non-luminescent (e.g., substantially non-luminescent) peptide and/or polypeptide units. In particular, bioluminescent activity is conferred upon a non-luminescent polypeptide via structural complementation with another, complementary non-luminescent peptide.

公开(公告)号：EP4219736A1

公开(公告)日：2023-08-02

申请号：EP22204685.6

申请日：2015-09-11

申请人： Promega Corporation

发明人： Dixon, Andrew S. ,  Encell, Lance P. ,  Machleidt, Thomas ,  Schwinn, Marie ,  Wood, Keith ,  Wood, Monika ,  Zimmerman, Kris

IPC分类号： C12Q1/66 ,  C12P21/04 ,  G01N33/53

摘要： Provided herein are substantially non-luminescent peptide/polypeptide tags that are inserted internally within a protein of interest or between N-terminal and C-terminal peptides/polypeptides. Interaction of the internally-inserted tag with a complement polypeptide/peptide that is also substantially non-luminescent results in the formation a bioluminescent reporter complex).