公开(公告)号：EP2459696B1

公开(公告)日：2017-11-08

申请号：EP10805905.6

申请日：2010-07-30

申请人： Qvella Corporation

发明人： TALEBOUR, Samad ,  KHINE, Aye Aye ,  ALAVIE, Tino ,  LEONARD, Stephan W ,  MAASKANT, Robert

IPC分类号： C12M1/42 ,  C12M1/33 ,  C12M1/34 ,  C12Q1/02 ,  C12Q1/68 ,  G01N27/26 ,  G01N33/50 ,  G01N33/543 ,  C12N11/00 ,  C12N13/00

CPC分类号： G01N33/5438 ,  B01L3/502715 ,  B01L3/502753 ,  B01L3/502761 ,  B01L2200/0647 ,  B01L2300/0645 ,  B01L2300/0809 ,  C12M23/16 ,  C12M25/00 ,  C12M25/04 ,  C12M29/10 ,  C12M35/02 ,  C12M41/38 ,  C12N1/066 ,  C12N11/14 ,  C12N13/00 ,  G01N33/5005

摘要： The present invention provides a microfluidic devices and methods of use thereof for the concentration and capture of cells. A pulsed non-Faradaic electric field is applied relative to a sample under laminar flow, which results to the concentration and capture of charged analyte. Advantageously, pulse timing is selected to avoid problems associated with ionic screening within the channel. At least one of the electrodes within the channel is coated with an insulating layer to prevent a Faradaic current from flowing in the channel. Under pulsed application of a unipolar voltage to the electrodes, charged analyte within the sample is moved towards one of the electrodes via a transient electrophoretic force.

公开(公告)号：EP2459696A1

公开(公告)日：2012-06-06

申请号：EP10805905.6

申请日：2010-07-30

申请人： Qvella Corporation

发明人： TALEBOUR, Samad ,  KHINE, Aye Aye ,  ALAVIE, Tino ,  LEONARD, Stephan W ,  MAASKANT, Robert

IPC分类号： C12M1/42 ,  C12M1/33 ,  C12M1/34 ,  C12Q1/02 ,  C12Q1/68 ,  G01N27/26 ,  G01N33/50 ,  G01N33/543 ,  C12N11/00 ,  C12N13/00

CPC分类号： G01N33/5438 ,  B01L3/502715 ,  B01L3/502753 ,  B01L3/502761 ,  B01L2200/0647 ,  B01L2300/0645 ,  B01L2300/0809 ,  C12M23/16 ,  C12M25/00 ,  C12M25/04 ,  C12M29/10 ,  C12M35/02 ,  C12M41/38 ,  C12N1/066 ,  C12N11/14 ,  C12N13/00 ,  G01N33/5005

摘要： The present invention provides a microfluidic devices and methods of use thereof for the concentration and capture of cells. A pulsed non-Faradic electric field is applied relative to a sample under laminar flow, which results to the concentration and capture of charged analyte. Advantageously, pulse timing is selected to avoid problems associated with ionic screening within the channel. At least one of the electrodes within the channel is coated with an insulating layer to prevent a Faradic current from flowing in the channel. Under pulsed application of a unipolar voltage to the electrodes, charged analyte within the sample is moved towards one of the electrodes via a transient electrophoretic force.

摘要翻译： 本发明提供微流体装置及其用于细胞浓缩和捕获的方法。 在层流下相对于样品施加脉冲非法拉第电场，其导致带电分析物的浓缩和捕获。 有利地，选择脉冲定时以避免与通道内的离子筛选相关的问题。 通道内的至少一个电极涂覆有绝缘层以防止法拉电流在通道中流动。 在向电极施加脉冲式单极电压的情况下，样品内的带电分析物通过瞬变电泳力朝向电极中的一个移动。