公开(公告)号：EP0954597A4

公开(公告)日：2002-05-29

申请号：EP96940775

申请日：1996-11-14

申请人： RAGLAND WILLIAM L ,  GOODWIN MARK A ,  NOVAK RENATA

发明人： RAGLAND WILLIAM L ,  GOODWIN MARK A ,  NOVAK RENATA

IPC分类号： C12Q1/68 ,  C12Q1/70 ,  C07H21/04

CPC分类号： C12Q1/6816 ,  C12Q1/6806 ,  C12Q1/6841 ,  C12Q1/6883 ,  C12Q1/701 ,  C12Q1/703 ,  C12Q2543/10 ,  C12Q2537/161 ,  C12Q2537/1373

摘要： The present invention provides a method for detecting the presence of a predetermined infectious agent in a human or animal subject comprising obtaining a preselected portion of a whole blood sample containing at least one intact blood cell from the subject and detecting the presence of a nucleic acid from the predetermined infectious agent in the intact blood cell by nucleic acid hybridization. The preselected portion of the blood sample can include any portion which contains at least one intact blood cell, for example a peripheral blood smear or cytospin buffy coat preparation. The intact blood cell can be any blood cell including, but not limited to white blood cells and red blood cells. In one embodiment, the blood cell is a lymphocyte.