公开(公告)号：EP1548107B1

公开(公告)日：2011-01-12

申请号：EP03792798.5

申请日：2003-08-22

申请人： RIKEN ,  Medical & Biological Laboratories Co., Ltd.

发明人： MIYAWAKI, Atsushi ,  ANDO, Ryoko ,  KARASAWA, Satoshi, Inalab ,  MIZUNO, Hideaki

IPC分类号： C12N15/09 ,  C07K14/35 ,  C12N1/21 ,  C07K19/00 ,  C12Q1/02 ,  G01N21/78 ,  G01N21/64

CPC分类号： G01N33/542 ,  C07K14/37 ,  C07K2319/00 ,  G01N33/533 ,  G01N33/582 ,  G01N2021/6439 ,  G01N2500/10

摘要： It is intended to provide a novel chromoprotein and novel fluoroproteins. Namely, a chromoprotein originating in Anthopleure inornata and having definite properties; and fluoroproteins originating in Trachyphyllia geoffroyi and Scolymia vitiensis and having definite fluorescent properties.

公开(公告)号：EP1548107A1

公开(公告)日：2005-06-29

申请号：EP03792798.5

申请日：2003-08-22

申请人： Riken ,  Medical & Biological Laboratories Co., Ltd.

发明人： MIYAWAKI, Atsushi ,  ANDO, Ryoko ,  KARASAWA, Satoshi, Inalab ,  MIZUNO, Hideaki

IPC分类号： C12N15/09 ,  C07K14/35 ,  C12N1/21 ,  C07K19/00 ,  C12Q1/02 ,  G01N21/78 ,  G01N21/64

CPC分类号： G01N33/542 ,  C07K14/37 ,  C07K2319/00 ,  G01N33/533 ,  G01N33/582 ,  G01N2021/6439 ,  G01N2500/10

摘要： It is an object of the present invention to provide a novel chromoprotein and a novel fluorescent protein. The present invention provides chromoproteins derived from Anthopleura inornata, which have certain property, and fluorescent proteins from Trachyphyllia geoffroyi and Scolymia vitiensis, which have certain fluorescent property.

摘要翻译： 本发明的目的是提供一种新型的生色蛋白和新的荧光蛋白。 本发明提供具有一定性质的源自Anthopleura inornata的衍生蛋白，以及具有一定荧光性质的来自Trachyphyllia geoffroyi和Scolymia vitiensis的荧光蛋白。

公开(公告)号：EP4321539A1

公开(公告)日：2024-02-14

申请号：EP22784517.9

申请日：2022-03-23

申请人： RIKEN ,  Tohoku University

发明人： MIYAWAKI, Atsushi ,  ANDO, Ryoko ,  HIRANO, Masahiko ,  TAKEDA, Noriyo

IPC分类号： C07K19/00 ,  C12M1/00 ,  C12M1/34 ,  C12N5/10 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N15/12 ,  C12N15/63 ,  C12Q1/02 ,  C12Q1/68 ,  C12Q1/6897 ,  G01N33/48 ,  G01N33/68 ,  A01K67/027 ,  C07K14/435

摘要： A novel fluorescent protein and uses thereof are provided. A polypeptide of the present invention has a fluorescence property, the polypeptide being indicated in any one of (1) to (3) below: (1) a polypeptide having an amino acid sequence set forth in SEQ ID NO: 1; (2) a polypeptide having an amino acid sequence set forth in SEQ ID NO: 1, the amino acid sequence having replaced, deleted, inserted, and/or added therein not less than 1 and not more than 32 amino acids; and (3) a polypeptide having not less than 85% sequence identity with respect to an amino acid sequence set forth in SEQ ID NO: 1.

公开(公告)号：EP3037534A1

公开(公告)日：2016-06-29

申请号：EP14838441.5

申请日：2014-08-22

申请人： Riken

发明人： MIYAWAKI, Atsushi ,  ANDO, Ryoko

IPC分类号： C12N15/09 ,  C07K14/435 ,  C07K19/00 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12Q1/02 ,  G01N33/48

CPC分类号： C07K14/435 ,  C07H21/04 ,  C07K2319/60 ,  C12N15/79 ,  C12N2510/00 ,  G01N33/582

摘要： A mode of a polypeptide according to the present invention exhibits a fluorescence property, and has (1) an amino acid sequence represented by SEQ ID NO. 1 or NO. 2, (2) an amino acid sequence represented by SEQ ID NO. 1 or NO. 2 in which amino acid sequence 1 to 34 amino acids have been replaced or otherwise modified, (3) a sequence identity of 85% or more with respect to the amino acid sequence represented by SEQ ID NO. 1 or NO. 2, or (4) an amino acid sequence encoded by a polynucleotide that hybridizes under a stringent condition with a polynucleotide having a sequence complementary to a polynucleotide that encodes the polypeptide defined in (1).

摘要翻译： 根据本发明的多肽的模式显示荧光性质，并且具有（1）SEQ ID NO：1所示的氨基酸序列。 1或否 2，（2）由SEQ ID NO： 1或否 2，其中氨基酸序列1至34个氨基酸已被替换或以其它方式修饰，（3）相对于SEQ ID NO：1所示的氨基酸序列，其序列同一性为85％或更高。 1或否 2，或（4）由严格条件下杂交的多核苷酸编码的氨基酸序列与具有与编码（1）中定义的多肽的多核苷酸互补的序列的多核苷酸编码。